LABORATORY EVALUATION OF BENGKULU ISOLATES OF Beauveria bassiana AND Metarhizium anisopliae AGAINST COFFEE BERRY BORER, Hyphotenemus hampei, USING SPRAYING METHOD

Abstract

Laboratory Evaluation of Local Isolates of Beauveria bassiana and Metarhizium anisopliae against Coffee Berry Borer, Hyphotenemus hampei, using spryaing method. Beauveria bassiana Bals (Vuillemin) and Metarhizium anisopliae (Metschnikoff) Sorokin are two species of fungi that have been studied widely and used as biological control of various insect pests, including coffee berry borer (CBB), Hyphotenemus hampei Ferrari. Laboratory studies were done in June – July 2017, to evaluate local isolates of both fungi species against CBB, the most destructive insect pest of coffee worldwide, including in Indonesia. B. bassiana was isolated from dead sweet potato weevil (Cylas formicarius) and green stink bug (Nezara viridula), and from soil. M. anisopliae was isolated from soil. The pure cultures of the fungi were mass-cultured on corn based media. The conidia harvested from 4 week colonies was hand sprayed directly upon CBB adults (females) and damaged coffee berry at the concentration of 109 ml-1. In additions, the conidia of B. bassiana isolated from C. formicarius alone was diluted to 106- 109 ml-1 and applied to damaged coffee berry. The results showed that when the conidia were applied directly upon the insects, the mortality of beetle were higher in B. bassiana than in M. anisopliae treatments. Pooled data indicated that B. bassiana isolated from death insects significantly caused higher mortality than did M. anisopliae isolated from soil. Beetle mortality was 76.7 and 80.0% for B. basiana, and 60.3 and 60% for M. anysopliae treatments. Application upon damage coffee berry indicated much lower mortality. Application of higher concentration upon damaged coffee berry resulted higher mortality, but data are not consistent. The mortality of CBB larvae was much less and negligible even at the highest conidia concentration.

Isolation and Characterization of Native Isolates of Metarhizium sp. as a Biocontrol Agent of Hypothenemus hampei in Rodríguez de Mendoza Province—Peru

“…The response varies due to the genetic diversity that exists within the genus, some of them with high pathogenicity and others not, as described by Mesquita et al [40], since among their isolated isolates, precisely the LCM S04 isolate stands out as the most efficient with a concentration of 10 7 , reaching values higher than 90% against R. microplus. On the other hand, Apriyanto and Nadrawati [41] reached lower results for isolated Metarhizium anisopliae, reaching a maximum efficacy of 80% with a concentration of 10 9 , which could be due to the method of exposure of the entomopathogenic fungus since immersion increases contact and spraying mimics the dispersion of conidia in the field.…”

Kemampuan Blastospora Paecilomyces fumosoroseus, Metarhizium anisopliae dan Lecanicillium decanii dalam Menginfeksi Kumbang Hypothenemus hampei

“…Pengendalian hama PBKo dengan memanfaatkan jamur patogen serangga (JPS) telah lama dikembangkan. Jamur Beauveria bassiana dan Metarhizium anisopliae telah digunakan sebagai agens hayati pengendali berbagai hama tanaman termasuk PBKo (Jaramillo, Montoya, Benavides, & Góngora B, 2015;Apriyanto & Nadrawati, 2019). Hasil penelitian Bustillo, Bernal, Benavides, & Chaves (1999), menunjukkan bahwa jamur B. bassiana dan M. anisopliae dalam bentuk konidia mampu menginfeksi kumbang H. hampei di laboratorium.…”

“…Hasil penelitian Bustillo, Bernal, Benavides, & Chaves (1999), menunjukkan bahwa jamur B. bassiana dan M. anisopliae dalam bentuk konidia mampu menginfeksi kumbang H. hampei di laboratorium. Hasil evaluasi infektifitas beberapa isolat B. bassiana dan M. anisopliae oleh Apriyanto & Nadrawati (2019) menunjukkan bahwa JPS tersebut mampu mematikan imago H. hampei rata-rata di atas 60%. Selain kedua jenis JPS tersebut, Paecilomyces lilacinus (Bustillo et al, 1999) dan Penicillium sp.…”

“…Hasil ini masih dibawah penelitian sebelumnya yang menggunakan konidia JPS. Pada kondisi laboratorium jamur B. bassiana dan M. anisopliae mampu mematikan imago PBKo, masingmasing 91% dan 94% (Jaramillo et al, 2015), 80% dan 60% (Apriyanto & Nadrawati, 2019) dan B. bassiana dengan konsentrasi 10 7 konidia/ml menyebabkan mortalitas 83% yang diaplikasikan sebelum infestasi dan 62% aplikasi setelah infestasi (Samuels, Pereira, & Gava, 2002). Berdasarkan laporan hasil-hasil penelitian sebelumnya menunjukkan bahwa infektifitas blastospora JPS lebih tinggi dibandingkan dengan konidianya.…”

The efficacy of botanical pesticides in controlling coffee berry borer, Hypothenemus hampei (Ferrari, 1867) (Coleoptera: Curculionidae): A meta-analysis

“…Nowadays, the use of synthetic chemical pesticides needs to be avoided as much as possible, and coffee farmers as well as agricultural practitioners are adopting more environmentally friendly methods, such as using biological control (Apriyanto & Nadrawati, 2019;Wagiyana et al, 2019), as well as the application of biopesticides (plant-based pesticides and microbial pesticides) (Sutriadi et al, 2019). Marrone (2019) stated that biopesticides are organic compounds and antagonistic microorganisms that inhibit or kill plant pests and diseases.…”