Laboratory Diagnostic Procedures for Human Brucellosis: An Overview of Existing Approaches

Etemadi, Afshar; Moniri, Rezvan; Neubauer, Heinrich; Goli, Yasaman Dasteh; Alamian, Saeed

doi:10.5812/jjm.91200

Cited by 8 publications

(9 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Confirmation by the real-time PCR technique for its sensitivity and specificity is therefore necessary. [19,33]. We also observed one case with clinical signs but was diagnosed negative.…”

Section: Discussionmentioning

confidence: 49%

See 1 more Smart Citation

New approach needed for diagnosis of human brucellosis in Morocco

et al. 2021

View full text Add to dashboard Cite

Human brucellosis is an infectious disease, caused by different species of the genus Brucella. It is a mandatory notifiable disease in most countries, including Morocco, and thus requires special vigilance on the part of health agencies. Prevention of this disease is based on surveillance and prevention of risk factors. Its management is based on serological or other diagnostic tests. The National Laboratory of Epidemic Diseases received 17 blood samples of human origin for notified cases of brucellosis in a region of southern Morocco. Biological confirmation of these samples was performed by Rose Bengal test and ELISA IgM test. The results showed a positivity rate of 88% proving the important role of serological tests in the diagnosis of brucellosis. Other rapid and efficient techniques are becoming essential for the confirmation of cases, taking into account the advanced biosafety procedures for these highly infectious pathogens.

show abstract

“…Confirmation by the real-time PCR technique for its sensitivity and specificity is therefore necessary. [19,33]. We also observed one case with clinical signs but was diagnosed negative.…”

Section: Discussionmentioning

confidence: 49%

“…They are inexpensive and less time consuming to perform and are easy to interpret [5]. Currently PCR facilitates a rapid, sensitive and specific detection and also allows to define species and biovar by amplification of specific regions [19].…”

Section: Introductionmentioning

confidence: 99%

New approach needed for diagnosis of human brucellosis in Morocco

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Despite the fact that culture is the Gold standard' in microbiological identification, culture can sometimes be wiped off by elements inherent to the bacterium itself despite careful laboratory procedures. The use of PCR to detect culture negative patients with brucellosis symptoms backed up this theory [7,25].…”

Section: Discussionmentioning

confidence: 99%

“…Serological assays are utilised for both the initial diagnosis of infection and the monitoring of treatment outcomes. After a successful treatment, the antibody titer should decrease [25].…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Human Brucellosis by Amplification Reaction of BCSP31 Gene in Egyptian Patients

Enany

Elghareeb

Zaghloul

et al. 2021

Alfarama Journal of Basic & Applied Sciences

View full text Add to dashboard Cite

Background: Human brucellosis is a major public health issue in Egypt. Because the clinical signs of human brucellosis are so varied and vague, scientific confirmation via organism isolation or antibody detection is required to confirm the diagnosis. The aim of this work is measurement of the sensitivity and specificity of PCR for the detection of Brucella abortus BCSP31gene compared with the blood culture methods.Methods: A total of 97 patients from different ages with symptoms of brucellosis were included in the study. This research study was carried out at Mansoura University Hospitals. The sensitivity and specificity of polymerase chain reaction (PCR) for the detection of BCSP31gene of Brucella abortus compared with blood culture methods.Results: In positive PCR cases of brucella (total 14), a single band of a 223-bp corresponding to BCSP31gene was obtained. The true positive brucellosis cases (n = 6) are positive of blood culture and PCR. PCR was sensitive than blood culture for diagnosis of brucellosis since the sensitivity and specificity of PCR are 87.5% and 98.73% and the sensitivity and specificity of blood culture are 84.62% and 96.15%. The positive and negative predictive values of PCR are 86.56% and 94.80% and the positive and negative predictive values of blood culture are 78.98% and 92.54%. The accuracy (%) of PCR and blood culture is 94.72% and 90.67%. The PCR assay was shown to be more sensitive and specific. Conclusion:The PCR showed higher efficiency for rapid diagnosis of human brucellosis, as compared to the blood culture method.

show abstract

“…Bacterial documentation was confirmed by the Matrix-Assisted Laser Desorption Ionization (MALDI-TOF MS), which is based on protein profile identification. This was reaffirmed by the bacterial detection via an automated biotyping system to improve detection of intracellular organisms, like Brucella [10]. However, Brucella speciation was not feasible given high rate of antisera cross-reactivity.…”

Section: Case Presentationmentioning

confidence: 99%

Brucella Shunt Infection Complicated by Peritonitis: Case Report and Review of the Literature

Al-Qarhi

Al-Dabbagh

2021

Infectious Disease Reports

View full text Add to dashboard Cite

Brucellosis is an endemic zoonotic disease in the Mediterranean basin and Middle East. The disease remains a diagnostic challenge due to an increasing trend of ambiguous and non-specific manifestations. We report a rare case of a 9-year-old boy who had a left frontotemporal arachnoid cyst with cystoperitoneal shunt who presented with fever for 2 weeks with gastrointestinal symptoms. He had no neurological manifestations. Diagnosis of Brucella shunt infection complicated with a peritoneal collection was established by isolation of the organism from cerebrospinal fluid (CSF) culture. Successful treatment was accomplished by shunt replacement and intravenous antibiotics followed by step-down oral therapy for an 18-month duration based on serological and radiological responses with no sequelae. We also reviewed the reported cases of CSF shunt infection in the literature for further guidance. Brucella shunt infection may be difficult to diagnose due to the diversity of signs and symptoms and the low yield on culture. Brucellosis should be always kept in mind in the differential diagnosis of patients living in endemic area with fever and non-specific symptoms. Diagnosis depends on a high index of suspicion. In addition to drug therapy, device replacement is advised to prevent treatment failure.

show abstract

Laboratory Diagnostic Procedures for Human Brucellosis: An Overview of Existing Approaches

Cited by 8 publications

References 65 publications

New approach needed for diagnosis of human brucellosis in Morocco

New approach needed for diagnosis of human brucellosis in Morocco

Diagnosis of Human Brucellosis by Amplification Reaction of BCSP31 Gene in Egyptian Patients

Brucella Shunt Infection Complicated by Peritonitis: Case Report and Review of the Literature

Contact Info

Product

Resources

About