“…For a long time, diagnosis of TB was dependent on Ziehl Neelsen smear microscopy with the light microscope [10]. The ZN based microscopy needs concentration of 5000 -10000 acid fast bacilli per ml for its detection [11,12]. Smear microscopy with the light microscope is a relatively less sensitive method for the diagnosis of TB [13,14] and can only detects 60-70% of the 1084 TB cases [15].…”