Laboratory Diagnosis of Tuberculosis

Pfyffer, Gaby E.

doi:10.1159/000066889

Cited by 4 publications

(1 citation statement)

References 37 publications

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“…Compared with culture and the clinical status, these methods have a high sensitivity and specificity in smear-positive specimens, but lower values are obtained in smear-negative specimens precluding their use as a screen to rule out the disease. For this reason, it has been recommended that molecular methods should always be interpreted in conjunction with the patient's clinical data [91].…”

Section: Nucleic Acid Amplification Methodsmentioning

confidence: 99%

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Palomino¹

2005

European Respiratory Journal

144

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Tuberculosis (TB) remains one of the major causes of death from a single infectious agent worldwide. Of great concern for TB control is the emergence of drug resistance. Since there is no cure for some multidrug-resistant strains of Mycobacterium tuberculosis, there is concern that they may spread around the world, stressing the need for additional control measures, such as new diagnostics, better drugs for treatment, and a more effective vaccine.Pulmonary TB can be diagnosed by its symptoms, chest radiography, sputum smear microscopy and by cultivation of M. tuberculosis, which is considered as the gold standard. Recent advances in molecular biology and molecular epidemiology, and a better understanding of the molecular basis of drug resistance in TB, have provided new tools for rapid diagnosis; however, the high cost of most of these techniques, and their requirement for sophisticated equipment and skilled personnel have precluded their implementation on a routine basis, especially in low-income countries.Other nonconventional diagnostic approaches proposed include the search for biochemical markers, detection of immunological response and early detection of M. tuberculosis by methods other than colony counting.In the present article, some of these approaches will be reviewed and the feasibility for their implementation in diagnostic laboratories will be discussed.

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Section: Nucleic Acid Amplification Methodsmentioning

confidence: 99%

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Palomino¹

2005

European Respiratory Journal

144

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Comparison of Conventional and Automated Culture System for Isolation of <i>Mycobacterium Tuberculosis</i>

Uddin¹,

Uddin

Mondol³

et al. 1970

J. Armed Forces Med. Coll.

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The Role of Polymerase Chain Reaction (PCR) in Diagnosis of Spine Tuberculosis after Pre-operative Anti-tuberculosis Treatment

Rudresha¹,

Ibrahim²,

Cc³

et al. 2011

MOJ

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Objective:The aim of this study was to evaluate the role of polymerase chain reaction (PCR) in the diagnosis of spinal tuberculosis after 2 weeks of preoperative anti-tuberculosis treatment and to compare PCR to the Löwenstein -Jensen Culture (LJC) and histopathological examination (HPE) methods. Methods: Twenty-five patients were included in this study. Sixteen patients were diagnosed and treated for spinal tuberculosis based on clinical and radiological evidence. Nine patients were controls. The LJC method and HPE of the specimen were performed according to hospital protocol. PCR was performed using primer encoding insertion of sequences IS6110 for mycobacterium tuberculosis complex. Clinical findings and radiological features were the gold standard for comparison. Results: PCR results were 15 positive and one negative. The sensitivity and specificity of PCR was 94% and 100% respectively (with 95% confidence interval [CI] 67% to 99% and 63% to 100%, respectively). HPE results showed 13 were positive and 3 negative in the spinal tuberculosis group; for the control group, all were negative. Sensitivity and specificity value of HPE was 82 % and 100% respectively (with 95% confidence interval [CI] 54% to 95% and 63% to 100%, respectively). Use of LJC showed only one was positive and 15 were negative in the spinal tuberculosis group whole all nine in the control group were negative. Sensitivity and specificity value of LJC was 6% and 100% respectively (with 95% confidence interval [CI] 0.3% to 32% and 63% to 100%, respectively). Conclusion: Our findings showed that the PCR for Mycobacterium tuberculosis is reliable as a method for diagnosis of spinal tuberculosis, even after of 2 weeks of anti-TB treatment, with an overall sensitivity of 94% and specificity of 100%.

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Laboratory Diagnosis of Tuberculosis

Cited by 4 publications

References 37 publications

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field

Comparison of Conventional and Automated Culture System for Isolation of <i>Mycobacterium Tuberculosis</i>

The Role of Polymerase Chain Reaction (PCR) in Diagnosis of Spine Tuberculosis after Pre-operative Anti-tuberculosis Treatment

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