Laboratory diagnosis of SARS-CoV-2 - A review of current methods

Mathuria, Jitendra Prasad; Yadav, Ramakant; Rajkumar, .

doi:10.1016/j.jiph.2020.06.005

Cited by 115 publications

(114 citation statements)

References 28 publications

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“…At present, with no specific therapeutic interventions or vaccines, COVID-19 has continued to cause a human and severe economic disruption affecting millions of people [ 6 ]. The disease pandemic and mortality showed the necessity for accurate and convenient approaches to diagnose, treat, and therapeutically monitor respiratory viral infections [ 7 , 8 ]. Several efforts have combined to rapidly develop a treatment and/or a vaccine to overcome the COVID-19 [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Risk and Prevalence of COVID-19 Infection in Colorectal Cancer Patients: a Systematic Review and Meta-analysis

Antikchi

Neámatzadeh

Ghelmani

et al. 2020

J Gastrointest Canc

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Background Patients with cancer might be at an increased risk of infection with COVID-19 and a more severe disease course. However, different tumor types have differing susceptibility to the infection and COVID-19 phenotypes. Thus, the risk and prevalence of COVID-19 is not uniform across the different tumor types. Here, we performed a meta-analysis to estimate the risk and prevalence of COVID-19 infection in colorectal cancer (CRC) patients. Methods A comprehensive literature search was performed up to July 25, 2020, thorough PubMed, Web of Science, Scopus, Google Scholar, CNKI, CBM, China Science, Wan Fang, and SciELO databases. The risk of COVID-19 infection in CRC patients was performed based on the odds ratios (ORs) and 95% confidence interval (95% CI). Results A total of six studies with 204 different cancer patients with COVID-19 and 92 CRC infected patients with COVID-19 were selected. Our results showed that the prevalence of COVID-19 infection in CRC patients was 45.1% in the global population. The pooled data showed that there is no a significant risk of infection with COVID-19 in CRC patients in the global population (OR = 0.261, 95% CI 0.099-0.533, p = 0.082). However, when subgroup analysis was performed based on country of origin, we found a significant correlation in Chinese CRC patients (OR = 0.221, 95% CI 0.146-0.319, p ≤ 0.001). Conclusions This study results revealed that Chinese CRC patients harbored a higher risk of COVID-19 infection. However, more multicenter, larger sample sizes and high-quality studies are required to verify this meta-analysis result.

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Section: Introductionmentioning

confidence: 99%

The Risk and Prevalence of COVID-19 Infection in Colorectal Cancer Patients: a Systematic Review and Meta-analysis

Antikchi

Neámatzadeh

Ghelmani

et al. 2020

J Gastrointest Canc

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“…The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.06.20244905 doi: medRxiv preprint primers and probe sets for virus detection has been evaluated, but there are still few studies that assess the sensitivity and specificity in the critical range of detection of the RT-PCR technique (Mathuria et al 2020). Among the most sensitive primer and probe sets are those for target N available by the US CDC (Vogels et al 2020, Etievant et al 2020 , .…”

Section: Discussionmentioning

confidence: 99%

Optimizing SARS-CoV-2 molecular diagnostic using N gene target: insights about reinfection

Klein

Barbosa

et al. 2020

Preprint

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IntroductionMolecular diagnosis of SARS-CoV-2 is a huge challenge to many countries around the world. The cost of tests to check infected people is inaccessible since specialized teams and equipment are not disposable in remote locations. Herein, we compared the fitness of two primers sets to the SARS-CoV-2 N gene in the molecular diagnosis of COVID-19.Materials and MethodsThe 1029 patient samples were tested to presense/abscence molecular test using in house US CDC protocol. We compared the fitness of two primers sets to two different regions of N gene targets.ResultsBoth targets, N1 and N2 displayed similar fitness during testing with no differences between Ct or measurable viral genome copies. In addition, we verified security ranges Cts related to positive diagnostic with Ct above 35 value failuring in 66,6% after retesting of samples.Main conclusionOur data suggest that it is secure to use just one primer set to the N gene to identify SARS-CoV-2 in samples and the labs should be careful to set positive samples in high Ct values using high cutoffs.

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“…Controversially, the molecular test of the same trademark was reported as highly accurate in the USA [9]. More information about PoC assays accuracy for Detection SARS-CoV-2 become available, and none of the published studies considers the non-ELISA PoC ones as adapted for detection of suspected patients either symptomatic or asymptomatic, due to the high risk of false negatives especially during the beginning days from the onset [10][11][12][13][14][15][16]. While some countries, specified and updated a pre-defined list of the approved Serological COVID-19 tests, based on the evaluation of their accuracy [6].…”

Section: Point Of Care (Poc) Serology Tests and Vaccination As Prmentioning

confidence: 99%

COVID-19 case management: The policy model in Morocco context

Bennis¹

2020

Preprint

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BackgroundBased on the updated scientific evidence around SARS-CoV-2 diagnostic, health policymakers had to consider that many decisions could enhance or limit the success of the overall COVID-19 control strategy. The purpose of this study is to share alternative COVID-19 case management based on the updated international knowledge.MethodsThis study presents the main information about COVID-19 case management in Morocco from March to October 2020. The NVivo qualitative model content analysis was used to compare and prioritize health decisions with updated scientific evidence.ResultsThe lack of molecular diagnostic accuracy using the interpretation of cycles quantification values, was targeted only by allowing all private laboratories to do RT qPCR. However, there is an urgent need for standardisation with accurate molecular SARS-CoV-2 thermocyclers and kits that notify systematic cycles quantification and do more tests per days to control the spread effectively. A predictive tree of the cycle’s range is presented following three steps: 1) the initial clinical definition, 2) the molecular confirmation, 3) and the diagnostic follows up results of the RT qPCR up to 28 days after the onset. At the same time, the seasonal vaccination against influenza and pneumonia could help to reduce COVID-19 deaths.ConclusionsUntil an available SARS-CoV-2 specific vaccine and/or curative effective treatment, updated control strategy in Morocco and similar context countries require to target population living in highly COVID-19 epidemic cities or areas by mass testing with the right interpretation of PCR values changes, associated to seasonal vaccination to foster the immunity.

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Laboratory diagnosis of SARS-CoV-2 - A review of current methods

Cited by 115 publications

References 28 publications

The Risk and Prevalence of COVID-19 Infection in Colorectal Cancer Patients: a Systematic Review and Meta-analysis

The Risk and Prevalence of COVID-19 Infection in Colorectal Cancer Patients: a Systematic Review and Meta-analysis

Optimizing SARS-CoV-2 molecular diagnostic using N gene target: insights about reinfection

COVID-19 case management: The policy model in Morocco context

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