Laboratory Diagnosis of Multiple Sclerosis: Immunological and Biochemical Aspects

Thompson, E J

doi:10.1093/oxfordjournals.bmb.a071391

Cited by 65 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This assumption is supported by such findings as the disfunction of T cell suppressor activity (3)(4)(5)(6)(7)(8)(9), the local synthesis of oligoclonal IgG in the CNS (6)(7)(8)(9), and the positive pattern of immunofluorescence suggesting IC deposition in the MS plaques (10,11). This assumption is supported by such findings as the disfunction of T cell suppressor activity (3)(4)(5)(6)(7)(8)(9), the local synthesis of oligoclonal IgG in the CNS (6)(7)(8)(9), and the positive pattern of immunofluorescence suggesting IC deposition in the MS plaques (10,11).…”

mentioning

confidence: 63%

“…Immunological events may be involved in the pathogenesis of MS. This assumption is supported by such findings as the disfunction of T cell suppressor activity (3)(4)(5)(6)(7)(8)(9), the local synthesis of oligoclonal IgG in the CNS (6)(7)(8)(9), and the positive pattern of immunofluorescence suggesting IC deposition in the MS plaques (10,11). These observations have stimulated research on the pattern of circulating IC in MS.…”

mentioning

confidence: 78%

See 1 more Smart Citation

Circulating immune complexes in serum and in cerebrospinal fluid of patients with multiple sclerosis

Procaccia¹,

Lanzanova²,

Caputo

et al. 1988

Acta Neurologica Scandinavica

View full text Add to dashboard Cite

We studied circulating immune complexes (IC) in the serum and cerebrospinal fluid (CSF) of patients with clinically defined multiple sclerosis (MS), in order to establish a correlation with the clinical course of the disease and to investigate the molecular composition of the IC isolated from patients in active phase of the disease. Serum IC levels were found to be significantly increased in patients from the progressive and active relapsing-remittent subgroups with both the CIC-conglutinin and C1q-binding methods. High levels of IC in CSF were detected only in the subgroup consisting of the relapsing-remittent patients in disease exacerbation when IC were determined by the C1q-binding test. No significant increase in serum or in CSF were found using the mRF-I test. The preliminary results of a qualitative investigation on serum IC in MS indicated that they are heterogeneous in nature, their size is mainly of the intermediate type, and they contain IgG, IgM, complement components and beta 2-microglobulins, the latter presenting an observation both new and interesting for studies on serum IC in MS patients.

show abstract

mentioning

confidence: 63%

mentioning

confidence: 78%

Circulating immune complexes in serum and in cerebrospinal fluid of patients with multiple sclerosis

Procaccia¹,

Lanzanova²,

Caputo

et al. 1988

Acta Neurologica Scandinavica

View full text Add to dashboard Cite

show abstract

“…Measurement of y-globulin by immunoprecipitation increased the yield of positive results to about 70"0.1 A further advance was made by Lowenthal2 and Kutt et al,3 who applied the technique of gel electrophoresis, and abnormalities have been reported in up to 94`0 of cases.4 Others have been less successful,' and it has been shown that concentration of the CSF-which was performed in all previous electrophoretic studies of MS-may of itself alter the electrophoretic pattern. 1 We have therefore modified the technique so that a satisfactory examination may now be performed on 0-5 ml of unconcentrated CSF. We report the prevalence of abnormalities in different groups of patients with MS classified according to the certainty of diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Oligoclonal immunoglobulins and plasma cells in spinal fluid of patients with multiple sclerosis.

Thompson¹,

Kaufmann²,

Shortman³

et al. 1979

BMJ

Self Cite

View full text Add to dashboard Cite

Summary and conclusionsA new modification of polyacrylamide gel electrophoresis (PAGE) was applied to cerebrospinal fluid proteins from patients with multiple sclerosis (MS The finding of oligoclonal bands provides valuable supporting evidence for the diagnosis of MS in the less definite clinical categories.protein and the other with 200 ug. The first gel was stained with 0-5 g Coomassie Blue/l and the second with 10 g naphthalene black/I. Gels were destained with 7% acetic acid. The gels were scanned in a Joyce Loebl densitometer. The immunoglobulins were identified (1) by displaying a higher relative affinity for Coomassie Blue than for naphthalene black (since they are basic proteins), and (2) by direct immunofixation of the gels with anti-IgG serum. 9 The figure gives the results of electrophoresis of CSF from a normal subject and from a patient with MS. The striking difference is in the y region, which shows a homogenous zone in the normal CSF but a series of diffuse, though clearly recognisable bands-in the CSF from the patient with MS-an oligoclonal pattern. It is important to distinguish the diffuse bands of the oligoclonal pattern from the sharp bands produced by haptoglobins resulting either from haemolysis in a specimen contaminated by blood or from a high total protein concentration.The total protein concentration was measured by nephelometry.Y0 The amount of IgG was determined by using the technique for radial immunodiffusion with Hyland low-level plates. Lange's colloidal gold est was performed.'0 Cells were centrifuged from 0-3 ml CSF with a Shandon cytocentrifuge

show abstract

“…It is well known that a normal cell count or a slight pleocytosis, with differential count showing mainly lymphocytes, is the typical finding in MS CSF (18,20). Less information has, however, been available regarding lympho cyte subpopulations, partly because of the low number of cells in the CSF.…”

Section: Discussionmentioning

confidence: 99%

Multiple Sclerosis

Naess¹,

Nyland²

1978

Eur Neurol

View full text Add to dashboard Cite

Peripheral blood from 21 patients with active and 19 patients with stable multiple sclerosis (MS), and cerebrospinal fluid (CSF) from 7 of the patients with active disease were examined for T lymphocytes by rosette formation with sheep erythrocytes. Increased numbers of T cells were found in CSF in active disease (94.0 ± 0.7 compared to 71.3 ± 4.4% in controls). Decreased relative and absolute numbers of T cells were found in peripheral blood in active, but not in stable disease (mean 47 ± 3 and 957 ± 93%/mm³ compared to 66 ± 2 and 1,381 ± 93%/mm³ in controls).

show abstract

Laboratory Diagnosis of Multiple Sclerosis: Immunological and Biochemical Aspects

Cited by 65 publications

References 0 publications

Circulating immune complexes in serum and in cerebrospinal fluid of patients with multiple sclerosis

Circulating immune complexes in serum and in cerebrospinal fluid of patients with multiple sclerosis

Oligoclonal immunoglobulins and plasma cells in spinal fluid of patients with multiple sclerosis.

Multiple Sclerosis

Contact Info

Product

Resources

About