Laboratory Diagnosis of Four Recent Sporadic Cases of Community-acquired SARS, Guangdong Province, China

Liang, Guodong; Chen, Qiuxia; Xu, Jianguo; Liu, Yufei; Lim, Wilina; Peiris, J. S. Malik; Anderson, Larry J.; Ruan, Li; Li, Hui; Kan, Biao; Di, Biao; Cheng, Peter K.C.; Chan, Kwok‐Hung; Erdman, Dean D.; Gu, Shi-Jian; Yan, Xiaojun; Liang, Weili; Zhou, Dun-Jin; Haynes, Lia M.; Duan, Shimiao; Zhang, Xin; Zheng, Han; Gao, Yang; Tong, Suxiang; Li, Dexin; Fang, Ling; Qin, Pengzhe; Xu, Wenbo

doi:10.3201/eid1010.040445

Cited by 125 publications

(111 citation statements)

References 14 publications

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“…This index patient also contacted house rats in his apartment a few days before disease onset. It is important to emphasize that, unlike most SARS patients during the 2002-2003 epidemic, these four new patients clinically presented very mild symptoms, and neither of them had close contacts who were infected (15). able to sequence nearly completely the SARS-CoV viral genome from the first two of the four human patients, the two palm civets of the Guangzhou food market, and one sample from the palm civet cage at the restaurant TDLR.…”

Section: Resultsmentioning

confidence: 99%

Cross-host evolution of severe acute respiratory syndrome coronavirus in palm civet and human

Song

Zhang

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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650

654

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Section: Resultsmentioning

confidence: 99%

Cross-host evolution of severe acute respiratory syndrome coronavirus in palm civet and human

Song

Zhang

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

650

654

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“…Once established in humans, the infection spread directly from person to person by aerosol or droplets. Although the future reemergence of SARS-CoV remains possible, the virus has not reappeared since the last documented case in 2003, except for sporadic (18) or lab-related (26) cases. However, two other new human coronaviruses, namely, CoV-NL63 (33), a group 1 coronavirus associated with bronchiolitis and lower respiratory tract infections, and CoV-HKU1 (35), a group 2 coronavirus associated with pneumonia, have been identified in the interval, and the previously known human coronaviruses 229E and OC43 continue to circulate.…”

mentioning

confidence: 99%

Antiviral Effects of Antisense Morpholino Oligomers in Murine Coronavirus Infection Models

Burrer¹,

Neuman²,

Ting³

et al. 2007

J Virol

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The recent emergence of novel pathogenic human and animal coronaviruses has highlighted the need for antiviral therapies that are effective against a spectrum of these viruses. We have used several strains of murine hepatitis virus (MHV) in cell culture and in vivo in mouse models to investigate the antiviral characteristics of peptide-conjugated antisense phosphorodiamidate morpholino oligomers (P-PMOs). Ten P-PMOs directed against various target sites in the viral genome were tested in cell culture, and one of these (5TERM), which was complementary to the 5 terminus of the genomic RNA, was effective against six strains of MHV. Further studies were carried out with various arginine-rich peptides conjugated to the 5TERM PMO sequence in order to evaluate efficacy and toxicity and thereby select candidates for in vivo testing. In uninfected mice, prolonged P-PMO treatment did not result in weight loss or detectable histopathologic changes. 5TERM P-PMO treatment reduced viral titers in target organs and protected mice against virus-induced tissue damage. Prophylactic 5TERM P-PMO treatment decreased the amount of weight loss associated with infection under most experimental conditions. Treatment also prolonged survival in two lethal challenge models. In some cases of high-dose viral inoculation followed by delayed treatment, 5TERM P-PMO treatment was not protective and increased morbidity in the treated group, suggesting that P-PMO may cause toxic effects in diseased mice that were not apparent in the uninfected animals. However, the strong antiviral effect observed suggests that with further development, P-PMO may provide an effective therapeutic approach against a broad range of coronavirus infections.

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“…The fourth incident (Guangzhou, Gouangdong province, China [18][19][20]) resulted in four sporadic, community-acquired cases arising over a 6-week period. Three of the cases were attributed to exposure to animal or environmental sources, whereas the source of exposure is unknown in the remaining case.…”

Section: Sarsmentioning

confidence: 99%

Postgraduate Course ERS Munich 2006 - Avian influenza and SARS: updates on the big stories

2006

Breathe

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Laboratory Diagnosis of Four Recent Sporadic Cases of Community-acquired SARS, Guangdong Province, China

Abstract: This document has been produced by the Severe Acute Respiratory Syndrome (SARS) Epidemiology Working Group and the participants at the Global Meeting on the Epidemiology of SARS, 16-17 May 2003.

Cited by 125 publications

References 14 publications

Cross-host evolution of severe acute respiratory syndrome coronavirus in palm civet and human

Cross-host evolution of severe acute respiratory syndrome coronavirus in palm civet and human

Antiviral Effects of Antisense Morpholino Oligomers in Murine Coronavirus Infection Models

Postgraduate Course ERS Munich 2006 - Avian influenza and SARS: updates on the big stories

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