LABORATORY DIAGNOSIS OF ACUTE HUMAN PARVOVIRUS B19 INFECTION BY SPECIFIC IgM DETECTION

Papular-purpuric "gloves and socks" syndrome due to parvovirus B19: report of a case with unusual features

“…These tests can produce false positive and false negative results, and IgM detection using native antigen in a capture assay format should be therefore performed as a confirmatory test. In acute B19 infection, the capture assay by enzyme immunoassay (MACEIA-FIOCRUZ) has been 100% concordant with the referential capture assay by radioimmunoassay (MACRIA-CPHL-UK) 16 .…”

“…Antistreptolysin O level in serum from day 29 of illness was 104 IU/mL. Serologic tests for parvovirus B19 were performed by an "in house" IgM capture enzyme immunoassay (MACEIA-FIOCRUZ) using native virus antigen 16 , and by immunofluorescence to detect B19-specific IgG antibody 17 . Both serum specimens yielded B19-specific IgM and IgG antibodies, confirming an acute B19 infection.…”

Clinical and epidemiological aspects of human parvovirus B19 infection in an urban area in Brazil (Niterói city area, State of Rio de Janeiro, Brazil)

“…All serum samples were tested for the presence of anti-rubella IgM virus antibodies by using a commercial enzyme immunoassay (EIA) (Rubenostika IgM, Organon), for anti-measles virus IgM by using an antibody capture EIA developed at the Centers for Disease Control and Prevention (Atlanta, USA) (Hummel et al 1992), and for anti-dengue virus IgM by using an in-house EIA (Kuno et al 1987, Nogueira et al 1992. Those specimens, negative for rubella, measles and dengue virus antibodies, were also tested for anti-human parvovirus B19 IgM using an antibody capture EIA (MACEIA) (Cubel et al 1994, Nascimento et al 1998. Primary infection with human herpesvirus type 6 (HHV-6) was diagnosed by an indirect immunofluorescence test for low avidity HHV-6 IgG (Ward et al 1989).…”

Anti-VP1 and anti-VP2 antibodies detected by immunofluorescence assays in patients with acute human parvovirus B19 infection

“…Other commercial assays using recom-binant baculovirus B19 antigens (VP2) were developed as enzyme immunoassay. These tests avoid the disadvantages of IFA such as operator interpretation (Nascimento et al 1998). Biotrin IgM IFA detection has an excellent specificity and sensitivity (Bruu & Nordbo 1995, Cohen & Bates 1995, Sloots & Devine 1996, and the corresponding IgG IFA can be used to determine IgG avidity (Gray et al 1993).…”