Laboratory Bioassay and Field Evaluation of Copper Gluconate as a New Potential Bactericide Against Xanthomonas campestris pv. citri (Hasse) Dye

Abstract

Laboratory bioassays and field trials were carried out to study the toxicity and effectiveness of copper gluconate (CG) against Xanthomonas campestris pv. citri (Hasse) in comparison with copper hydroxide (CH) and DT (copper succinate + copper glutarate + copper adipate). The results of laboratory experiment indicated that CG recorded significant control against the bacteria at the concentration of 40 g/L or more. In particular, CG had a similar result to those of CH at the concentration of 100 g/L. Meanwhile, field experiment confirmed that CG had a similar efficacy compared with those of CH, DT, and Mancozeb. It was suggested that CG (1: 1000 dilutions) could be used as effective bactericide for managing citrus canker.

Copper Gluconate as an Ecofriendly Compound Protecting Apple and Orange Trees against the Most Common Pathogens Affecting Them during the Pre-harvest Stages

“…In other studies, carried out against Xanthomonas campestris pv. In both laboratory and field conditions indicated that Cu gluconate recorded significant control against the bacteria, with similar results as Cu hydroxide and mancozeb treatments [16] . Cu gluconate was able to inhibit the growth of the fungus Curvularia eragrostidis in in vitro assays [17] .…”

Prevalence of Xanthomonas euvesicatoria (formally X. perforans) associated with bacterial spot severity in Capsicum annuum crops in South Central Chihuahua, Mexico

“…For minimum inhibitory concentration (MIC) analysis, antimicrobial dilutions were prepared at 4, 8, 16, 32, 64, 128, 256, 512, and 640 µg/mL in tryptone soy broth (TSB). Microbial resistance was defined by Xanthomonas growth on copper compounds at 200 µg/mL (Zhang et al, 2013;Roach et al, 2020), on Ox at 25 µg/mL (Farfán, Benítez & Carvajal, 2014), and on gentamicin at 25 µg/mL (Rojas, Peña & Peña-Vera, 2019). In addition, pH values from all the antimicrobial tested and their dilutions were determined.…”

Elucidating the mechanism of action of copper heptagluconate on the plant immune system against Pseudomonas syringae in tomato (Solanum lycopersicum L)

“…The study of new compounds based on organic formulations of Cu and their dual effect as an inductor of plant innate defences would provide a valuable alternative to farmers for the control of pathogens such as P. syringae . Cu heptagluconate is characterized by great Cu absorption and diffusion over the plant, and is safer for the environment than other traditional inorganic Cu‐based fungicides . Nevertheless, biochemical studies elucidating the mode of action of Cu heptagluconate against Pst, and its compatibility and lack of phytotoxicity in tomato have not been performed to date.…”

“…Cu heptagluconate is characterized by great Cu absorption and diffusion over the plant, and is safer for the environment than other traditional inorganic Cu-based fungicides. 15 Nevertheless, biochemical studies elucidating the mode of action of Cu heptagluconate against Pst, and its compatibility and lack of phytotoxicity in tomato have not been performed to date. Therefore, the main goal of this study was to elucidate the efficacy of Cu in enhancing the plant immune system in tomato against Pst.…”