Laboratory and field evaluations of the efficacy of a fast-killing baculovirus isolate from Spodoptera frugiperda

Behle, Robert W.; Popham, Holly J.R.

doi:10.1016/j.jip.2011.11.002

Cited by 45 publications

(37 citation statements)

References 31 publications

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“…Unfortunately, scientific and public aversion to field release of genetically modified organisms has stymied the potential for commercialization of these recombinant baculoviruses (16). Recently, to circumvent this issue, a novel recombinant baculovirus (Bactrus) was constructed by the insertion of the Bt toxin gene between the two polyhedrin genes under the control of the polyhedrin promoter (17).…”

mentioning

confidence: 99%

NeuroBactrus, a Novel, Highly Effective, and Environmentally Friendly Recombinant Baculovirus Insecticide

Shim

Choi

Wang

et al. 2013

Appl Environ Microbiol

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A novel recombinant baculovirus, NeuroBactrus, was constructed to develop an improved baculovirus insecticide with additional beneficial properties, such as a higher insecticidal activity and improved recovery, compared to wild-type baculovirus. For the construction of NeuroBactrus, the Bacillus thuringiensis crystal protein gene (here termed cry1-5) was introduced into the Autographa californica nucleopolyhedrovirus (AcMNPV) genome by fusion of the polyhedrin-cry1-5-polyhedrin genes under the control of the polyhedrin promoter. In the opposite direction, an insect-specific neurotoxin gene, AaIT, from Androctonus australis was introduced under the control of an early promoter from Cotesia plutellae bracovirus by fusion of a partial fragment of orf603. The polyhedrin-Cry1-5-polyhedrin fusion protein expressed by the NeuroBactrus was not only occluded into the polyhedra, but it was also activated by treatment with trypsin, resulting in an ϳ65-kDa active toxin. In addition, quantitative PCR revealed that the neurotoxin was expressed from the early phase of infection. NeuroBactrus showed a high level of insecticidal activity against Plutella xylostella larvae and a significant reduction in the median lethal time against Spodoptera exigua larvae compared to those of wild-type AcMNPV. Rerecombinant mutants derived from NeuroBactrus in which AaIT and/or cry1-5 were deleted were generated by serial passages in vitro. Expression of the foreign proteins (B. thuringiensis toxin and AaIT) was continuously reduced during the serial passage of the NeuroBactrus. Moreover, polyhedra collected from S. exigua larvae infected with the serially passaged NeuroBactrus showed insecticidal activity similar to that of wild-type AcMNPV. These results suggested that NeuroBactrus could be recovered to wild-type AcMNPV through serial passaging.

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mentioning

confidence: 99%

NeuroBactrus, a Novel, Highly Effective, and Environmentally Friendly Recombinant Baculovirus Insecticide

Shim

Choi

Wang

et al. 2013

Appl Environ Microbiol

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“…The egt gene encodes an ecdysteroid UDP-glucosyltransferase that can extend the infection period allowing increased production of progeny OBs in each infected insect [31]. Using a fast-killing phenotype for pest control has been considered advantageous, since pest feeding damage on plants is reduced as larvae die earlier [32]. Nine defective SfCOL genotypic variants lacked the egt and sf27 genes, however only six of them presented a faster killing phenotype compared to SfCOL-wt, suggesting that other factors are likely involved in these phenotypes.…”

Section: Discussionmentioning

confidence: 99%

Deletion Genotypes Reduce Occlusion Body Potency but Increase Occlusion Body Production in a Colombian Spodoptera frugiperda Nucleopolyhedrovirus Population

et al. 2013

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A Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a mixture of different genotypes. To evaluate the insecticidal properties of the different genotypic variants, 83 plaque purified virus were characterized. Ten distinct genotypes were identified (named A through J). SfCOL-A was the most prevalent (71±2%; mean ± SE) showing a PstI restriction profile indistinguishable to that of SfCOL-wt. The remaining nine genotypes presented genomic deletions of 3.8 - 21.8 Kb located mainly between nucleotides 11,436 and 33,883 in the reference genome SfMNPV-B, affecting the region between open reading frames (ORFs) sf20 and sf33. The insecticidal activity of each genotype from SfCOL-wt and several mixtures of genotypes was compared to that of SfCOL-wt. The potency of SfCOL-A occlusion bodies (OBs) was 4.4-fold higher than SfCOL-wt OBs, whereas the speed of kill of SfCOL-A was similar to that of SfCOL-wt. Deletion genotype OBs were similarly or less potent than SfCOL-wt but six deletion genotypes were faster killing than SfCOL-wt. The potency of genotype mixtures co-occluded within OBs were consistently reduced in two-genotype mixtures involving equal proportions of SfCOL-A and one of three deletion genotypes (SfCOL-C, -D or -F). Speed of kill and OB production were improved only when the certain genotype mixtures were co-occluded, although OB production was higher in the SfCOL-wt isolate than in any of the component genotypes, or mixtures thereof. Deleted genotypes reduced OB potency but increased OB production of the SfCOL-wt population, which is structured to maximize the production of OBs in each infected host.

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“…• Chemical control (Dequech et al, 2013;Abrahams et al, 2017) • Biological control: -Effective natural enemies occur in the Americas and could be considered as candidates for Classical Biological Control (Abrahams et al, 2017) -Different entomopathogens have been screened and could be effectively used as bioinsecticides to control this pest (Barrera et al, 2011;Behle and Popham, 2012) • Biotechnological control: GMOs,, use of semiochemicals (pheromone)…”

Section: Control Methodsmentioning

confidence: 99%

Pest categorisation of Spodoptera frugiperda

Jeger¹,

Bragard²,

Caffier³

et al. 2017

EFS2

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The European Commission requested EFSA to conduct a pest categorisation of Spodoptera frugiperda (Lepidoptera: Noctuidae) a pest with hosts in 27 plant families. Favoured hosts include maize, rice and sorghum (Poaceae). Hosts also include crops within the Brassicaceae, Cucurbitaceae, Solanaceae, Rutaceae and other families. S. frugiperda is a taxonomic entity with reliable methods for identification. It is regulated in the EU as a harmful organism whose introduction into the EU is banned. It is native to tropical and subtropical regions of the Americas and migrates to temperate regions in North and South America during the summer. Establishment in temperate areas is prevented by its inability to overwinter. S. frugiperda has been intercepted on plant produce entering the EU. Phytosanitary measures are available to impede entry via traded commodities. In 2016, S. frugiperda was reported damaging maize in Africa. Subsequent reports indicate that it continues to spread severely damaging maize and other crops. If S. frugiperda spreads into north Africa, the likelihood of adults migrating into the temperate EU increases. Within the scope and level of analysis appropriate for pest categorisation, the EFSA Plant Health Panel concludes that S. frugiperda could establish in a small area of the southern EU from where it is likely to enter more northern regions forming transient summer populations, particularly in maize growing regions where impacts on yield could occur. However, uncertainties regarding establishment remain. Considering the criteria within the remit of EFSA to assess as regards status as a potential Union quarantine pest (QP) or as a potential regulated non-quarantine pest (RNQP), S. frugiperda satisfies the criteria to be regarded a Union QP but does not meet the criteria of (i) occurring in the EU territory, and (ii) plants for planting being the principal means of spread, criteria required for RNQP status.

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Laboratory and field evaluations of the efficacy of a fast-killing baculovirus isolate from Spodoptera frugiperda

Cited by 45 publications

References 31 publications

NeuroBactrus, a Novel, Highly Effective, and Environmentally Friendly Recombinant Baculovirus Insecticide

NeuroBactrus, a Novel, Highly Effective, and Environmentally Friendly Recombinant Baculovirus Insecticide

Deletion Genotypes Reduce Occlusion Body Potency but Increase Occlusion Body Production in a Colombian Spodoptera frugiperda Nucleopolyhedrovirus Population

Pest categorisation of Spodoptera frugiperda

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