Laboratory analyses of the potential toxicity of sediment‐associated polydimethylsiloxane to benthic macroinvertebrates

Henry, Kevin; Wieland, Willemien H.; Powell, David E.; Giesy, John P.

doi:10.1002/etc.5620201129

Cited by 8 publications

(1 citation statement)

References 28 publications

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“…A custom organoid well construct was fabricated using the transparent, biocompatible polymer PDMS (Sylgard 184, Dow Corning, USA) [ 33 ]. Briefly, the PDMS pre-polymer mixed with its curing agent (10:1 by weight) was degassed, poured into 35mm Petri dishes and cured at 60°C on a hotplate overnight to ensure full crosslinking.…”

Section: Methodsmentioning

confidence: 99%

Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy

et al. 2018

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Screening cells for their differentiation potential requires a combination of tissue culture models and imaging methods that allow for long-term tracking of the location and function of cells. Embryonic kidney re-aggregation in vitro assays have been established which allow for the monitoring of organotypic cell behaviour in re-aggregated and chimeric renal organoids. However, evaluation of cell integration is hampered by the high photonic load of standard fluorescence microscopy which poses challenges for imaging three-dimensional systems in real-time over a time course. Therefore, we employed light sheet microscopy, a technique that vastly reduces photobleaching and phototoxic effects. We have also developed a new method for culturing the re-aggregates which involves immersed culture, generating organoids which more closely reflect development in vivo. To facilitate imaging from various angles, we embedded the organoids in a freely rotatable hydrogel cylinder. Endpoint fixing and staining were performed to provide additional biomolecular information. We succeeded in imaging labelled cells within re-aggregated kidney organoids over 15 hours and tracking their fate while simultaneously monitoring the development of organotypic morphological structures. Our results show that Wt1-expressing embryonic kidney cells obtained from transgenic mice could integrate into re-aggregated chimeric kidney organoids and contribute to developing nephrons. Furthermore, the nascent proximal tubules that formed in the re-aggregated tissues using the new culture method displayed secretory function, as evidenced by their ability to secrete an organic anion mimic into the tubular lumen.

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Section: Methodsmentioning

confidence: 99%

Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy

et al. 2018

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Silicones

Greene,

House‐Knight,

Gentry

et al. 2023

Patty's Toxicology

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Linear polydimethylsiloxanes (PDMS), often commonly referred to as “silicones,” “siloxanes,” “organosilicones,” or “organosiloxanes,” are polymeric organosilicon substances composed of dimethylsiloxy units with trimethylsilyl end groups. This class of PDMS may best be represented by the main CAS number [ 63148‐62‐9 ], although other CAS numbers [( 9016‐00‐6 ) and ( 63394‐02‐5 )] may be considered representative of this class of compounds. PDMS are manmade organosilicon compounds that range from low molecular weight volatile materials to high molecular weight polymeric materials. Silicones have a wide range of applications found in all areas of everyday life. The chemical, rubber, plastics, and construction industries make up the majority of the total market volume. Results from studies conducted in animals following oral, dermal, and inhalation exposure indicated minimal adverse effects, with no significant increase reported in mortality or malignant or benign tumors. Due to the wide use of PDMS fluids in foods, drugs, cosmetics, and implants studies in humans have been conducted. The overall weight of medical and scientific evidence supports the conclusion that PDMS fluids used in food, drugs, and cosmetics, as well as in implants, at the concentrations currently in the marketplace, are not associated with an increased risk of adverse health effects.

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Photo‐Fenton Remediation of Wastewaters Containing Silicones: Experimental Study and Neural Network Modeling

Teixeira

Guardani

Nascimento³

2004

Chem Eng & Technol

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The present work is aimed at the investigation of the photo-Fenton technology with regard to the remediation of diluted aqueous emulsions containing an aminosilicone polymer, in a bench-scale photochemical reactor. The experimental results show a strong interaction between temperature, light, Fe(II) and H 2 O 2 concentrations on the degradation process, which generates substances that might be readily biodegradable and/or a solid phase that is easily separated by simple mechanical operations. The neural network technique is an effective, simple approach to successfully modeling the photo-Fenton degradation system, in which thermal and photochemical reactions and related phenomena (such as solid precipitation) take place. The model might therefore be useful in process optimization, as well as in the design and scaleup of photochemical reactors for industrial application.

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Laboratory analyses of the potential toxicity of sediment‐associated polydimethylsiloxane to benthic macroinvertebrates

Cited by 8 publications

References 28 publications

Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy

Ex vivo live cell tracking in kidney organoids using light sheet fluorescence microscopy

Silicones

Photo‐Fenton Remediation of Wastewaters Containing Silicones: Experimental Study and Neural Network Modeling

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