Laboratory-acquired Brucellosis

Noviello, Stephanie; Gallo, Richard; Kelly, Molly E; Limberger, Ronald J.; DeAngelis, Karen J.; Cain, Louise; Wallace, Barbara J.; Dumas, Nellie B.

doi:10.3201/eid1010.040076

Cited by 84 publications

(77 citation statements)

References 13 publications

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“…Although, MRT is considered as an ideal method for detecting infected cow and diagnosis of brucellosis in individual animals (Noriello, 2004), it may be giving false positive reactions in colostrum or milk at the end of the lactation period and milk from cows suffering from a hormonal disorder or mastitis (Bercovich and Moerman, 1979).…”

Section: Resultsmentioning

confidence: 99%

“…Milk ring test (MRT) is a field test used for diagnosis and detection of brucella antibodies in milk and considered principle methods for detecting infected herds and diagnosis of brucella in individual animal (Noriello 2004). Rose Bengal plate test (RBPT) is based on agglutination of colored particulate antigen (killed Brucella organisms) by the antibodies present in the patient's serum (Chachra et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals

Nofal¹,

El-Leboudy²,

El-Makarem³

et al. 2017

AJVS

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Key words:Brucella species, RBPT, MRT, bcsp-31 gene Brucellosis is a worldwide zoonotic disease that cause major public health hazard in Egypt. The present work was conducted to investigate the prevalence of bovine brucellosis on the basis of serological test RBPT and MRT in serum and milk samples of lactating dairy animals, also isolation of brucella organism from milk samples, PCR amplification was done for detection of Brucella abortus DNA using bcsp-31 target gene. 219 serum samples from sheep and goat and 119 milk samples from cow, sheep and goat were collected from governmental and private farm. The prevalence of brucellosis by RBPT were 34.5 and 61.4% in serum samples of sheep and goat while by MRT were 2.33, 13.8 and 10% in milk samples of cow, sheep and goat, respectively. The same prevalence was obtained when milk samples were cultured on the Brucella agar medium. A specific PCR assay targeting the bcsp-31 gene within the genome of Brucella species has been used and gave an amplicon of 223 bp in three strain of isolated brucella. It is concluded that RBPT and MRT used as screening tests for detection the prevalence of brucella species in serum and milk samples, PCR assay consider as a useful tool for detection Brucella species in milk.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals

Nofal¹,

El-Leboudy²,

El-Makarem³

et al. 2017

AJVS

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“…Özellikle bakteriyel süspan-siyonlar n santrifüjlenmesi ve vortekslenmesi gibi rutin bakteriyolojik prosedürler ve katalaz testi gibi biyokimyasal testlerin uygulanmas tehlikeli aerosol olu umuna yol açarak laboratuvar çal anlar na tehlike arz etmektedir (13). Vakalar n ço unda bula tan aerosoller sorumludur (14). Santrifüj tüplerinin veya kan-kültür i elerinin k r lmas laboratuvar ile ili kili hastal kta minör bir role sahiptir ve vakalar n sadece %20'sinden sorumludur (12,15).…”

Section: Brucella Türleriunclassified

Sağlık Çalışanlarında Enfeksiyon Riskleri ve Korunma II: Solunum Yoluyla Bulaşan Enfeksiyonlar

KARAHAN¹

2014

Ankara Üniversitesi Tıp Fakültesi Mecmuası

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Sa l k çal anlar n n çal t klar ortamda maruz kald klar en önemli mesleki hastal k ve ölüm nedeni enfeksiyonlard r. Enfeksiyon etkenlerinin sa l k personeline bula nda solunum yolu, kan ve di er vücut s v lar yla temastan sonra ikinci s rada yer almaktad r. Sa l k çal anlar na hava yoluyla bula an en önemli etkenlerden biri Mycobacterium tuberculosis olup sa l k çal -an nda tüberküloz görülme s kl ülkelerin geli mi li inin de bir göstergesi olarak kabul edilmektedir. Solunum yoluyla bula an di er önemli etkenler influenza virüsü ve laboratuvar çal anlar için Brucella türleridir. Bu derlemede sa l k çal anlar nda solunum yolu ile bula an enfeksiyon riskleri ele al nm ve bu risklerden korunma yollar na de inilmi tir.Anahtar Sözcükler: Sa l k personeli, enfeksiyon, etyoloji, korunma.The most important cause of occupational disease and mortality among healthcare workers is infections. Respiratory system is the second most frequent route for the transmission of infectious agents to healthcare workers following exposure to blood and other body fluids. Mycobacterium tuberculosis is one of the most important agents transmitted by airway to healthcare workers and the prevalance of tuberculosis in healthcare workers is regarded as a criteria for the development level of countries. Other agents transmitted by respiratory route are influenza virus and Brucella species for laboratory workers. In this review, infection risks transmitted by airway are covered and ways of protection are briefly discussed.Key Words: Healthcare worker, infection, ethiology, protection.Sa l k çal anlar n n meslek riskleri aras nda yer alan solunum yolu ile bulaan enfeksiyonlar önemli bir i gücü kayb nedenidir. Hastanelerde hava yoluyla bula an enfeksiyonlar, çevre-sel rezervuarlardan herhangi bir yolla (insanlar, hava ak m , su, in aat malzemeleri, cihazlar vb.) hastaneye giren mikroorganizmalar n, hastanede uygun bir ekolojik ortamda üremesi ve sonra havaya kar arak bir enfeksiyon kayna olu turmas yla geli mektedir (1).Solunum yolu enfeksiyonlar , damlac klar arac l yla (direkt bula ) veya damlac k çekirdekleri içindeki patojenlere maruziyet sonucu (indirekt bula ) olu ur. Damlac klar, enfekte hastalar n hap rma veya öksürmeleriyle oluan 5 μm'den büyük enfeksiyöz partiküllerdir ve kaynak ki inin yakla k 1 m uza na kadar yay labilir. Konjonktival, nazal veya oral mukozaya damlac klar n temas sonucu bula gerçek-le ir. nfluenza virüs, rinovirüsler, adenovirüsler ve respiratuvar sinsityal virüs (RSV) bu tür yay lan patojenlere örnek olarak verilebilir. Bu mikroorganizmalar n esas olarak direkt yayl mla bula mas ve damlac klar n havadan h zl bir ekilde yere dü me e ilimleri nedeniyle, hastanelerdeki hava ak m kontrol tedbirleri bu enfeksiyonlar önlemede yeterli olmayabilir (1).Damlac k çekirdekleri, havada as l kalan damlac klar n kuruyarak olu turdu u, 1-5 μm büyüklü ündeki partiküller-dir. Bu partiküller; kuru bir sekresyon örtüsü ile korunan potansiyel olarak canl mikroorganizmalar ta yabilir, havada uzun süre as l kalabilir ve hava ak m ile uzak m...

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“…infections [10] and its use as a potential agent of bioterrorism is of concern [2]. In this case, the Brucella isolate was misidentified as B. zoohelcum by the MicroScan Walk-away, and was identified as B. melitensis by the Vitek 2 system.…”

Section: Figure Phylogenetic Tree Of Brucella Melitensismentioning

confidence: 99%

16S rRNA gene sequence analysis of a Brucella melitensis infection misidentified as Bergeyella zoohelcum

Dash

Panigrahi

Al-Zarouni

et al. 2011

J Infect Dev Ctries

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Misidentification of Brucella species from clinical specimens using commercial bacterial identification systems is a recurring problem. An isolate from a bacterimic patient was identified as Bergeyella zoohelcum by MicroScan Walk-Away (Siemens Healthcare Diagnostics Inc., West Sacramento, CA, USA) and as Brucella melitensis by Vitek 2 system (bioMérieux Inc., Durham, NC, USA). Because of this identification ambiguity by the two automated bacterial identification systems we performed 16S rRNA sequencing and serotyping of the isolate and confirmed it as a Brucella spp. Combining the sequence data with the Vitek 2 system data we conclude that the infection was caused by B. melitensis.

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Laboratory-acquired Brucellosis

Cited by 84 publications

References 13 publications

Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals

Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals

Sağlık Çalışanlarında Enfeksiyon Riskleri ve Korunma II: Solunum Yoluyla Bulaşan Enfeksiyonlar

16S rRNA gene sequence analysis of a Brucella melitensis infection misidentified as Bergeyella zoohelcum

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