Labelling of Granulocytes by Phagocytic Engulfment with 64Cu-Labelled Chitosan-Coated Magnetic Nanoparticles

A, Pala; Liberatore, M.; D’Elia, Piera; Nepi, Fabio; Megna, Valentina; Mastantuono, M.; Al‐Nahhas, Adil; Rubello, Domenico; Barteri, Mario

doi:10.1007/s11307-011-0526-y

Cited by 17 publications

(17 citation statements)

References 23 publications

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“…There are occasional reports on using other particles, e.g. gold nanoparticles for CT imaging (74), manganese particles for MR imaging (75) or positron labeled particles for PET imaging as in the case of 64 Co-labeled chitosan nanoparticles (76) but the most widely used particles have been either gadolinium (paramagnetic) or iron oxide (superparamagnetic) nanoparticles for MR imaging (65). A weakness of using standard paramagnetic agents is that they do not cause a change in MR signal that is large enough to allow visualization of relatively small numbers of cells.…”

Section: Nanoparticles As Diagnostic Probesmentioning

confidence: 99%

Nanoparticles for Imaging: Top or Flop?

Kießling¹,

Mertens²,

Grimm³

et al. 2014

Radiology

192

146

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Nanoparticles are frequently suggested as diagnostic agents. However, except for iron oxide nanoparticles, diagnostic nanoparticles have been barely incorporated into clinical use so far. This is predominantly due to difficulties in achieving acceptable pharmacokinetic properties and reproducible particle uniformity as well as to concerns about toxicity, biodegradation, and elimination. Reasonable indications for the clinical utilization of nanoparticles should consider their biologic behavior. For example, many nanoparticles are taken up by macrophages and accumulate in macrophage-rich tissues. Thus, they can be used to provide contrast in liver, spleen, lymph nodes, and inflammatory lesions (eg, atherosclerotic plaques). Furthermore, cells can be efficiently labeled with nanoparticles, enabling the localization of implanted (stem) cells and tissue-engineered grafts as well as in vivo migration studies of cells. The potential of using nanoparticles for molecular imaging is compromised because their pharmacokinetic properties are difficult to control. Ideal targets for nanoparticles are localized on the endothelial luminal surface, whereas targeted nanoparticle delivery to extravascular structures is often limited and difficult to separate from an underlying enhanced permeability and retention (EPR) effect. The majority of clinically used nanoparticle-based drug delivery systems are based on the EPR effect, and, for their more personalized use, imaging markers can be incorporated to monitor biodistribution, target site accumulation, drug release, and treatment efficacy. In conclusion, although nanoparticles are not always the right choice for molecular imaging (because smaller or larger molecules might provide more specific information), there are other diagnostic and theranostic applications for which nanoparticles hold substantial clinical potential.

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Section: Nanoparticles As Diagnostic Probesmentioning

confidence: 99%

Nanoparticles for Imaging: Top or Flop?

Kießling¹,

Mertens²,

Grimm³

et al. 2014

Radiology

192

146

View full text Add to dashboard Cite

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“…Because of the size (<0.5 µm) and shape of the CN and their ability to interact with the cell membrane resulting from their surface charge, it is likely that cellular internalisation involves an endocytosis or a phagocytosis pathway . These results reflect fast initial adhesion of [ 89 Zr]‐ or [ 64 Cu]‐loaded CN to the cell membrane followed by partial release leading to only a fraction of nanoparticles being internalised into the cell.…”

Section: Discussionmentioning

confidence: 99%

“…The uptake mechanism of [ 89 Zr]‐ or [ 64 Cu]‐loaded CN into cells is unclear. There is a possibility of phagocytic engulfment or internalisation via an endocytosis or pinocytosis mechanism based on the size, shape and surface charge of the CN …”

Section: Discussionmentioning

confidence: 99%

“…However, the sensitivity of PET (10 −11 –10 −12 M) is at least 1–2 orders of magnitude higher than single photon imaging systems (10 −10 M), and PET is more attractive for the quantification of the regional signal from migrated cells. Therefore, in vivo imaging of leukocyte trafficking using PET is receiving growing interest for applications in immunological studies to (i) track the selective recruitment of specific immune cells during pathogenesis, (ii) detect probable infectious/inflammatory foci and (iii) devise rational therapeutic strategies and carry out longitudinal studies.…”

Section: Introductionmentioning

confidence: 99%

“…Different approaches have been developed for tracking radiolabelled leukocytes in vivo with PET using the positron emitting isotopes copper‐64 ( 64 Cu, t 1/2 = 12.7 h) and fluorine‐18 ([ 18 F], t 1/2 = 109.7 min). Cationic [ 64 Cu] 2+ requires a chelate to transport it into cells; [ 64 Cu]‐pyruvaldehyde‐bis(N 4 ‐methylthiosemi‐carbazone) (PTSM), [ 64 Cu]‐polyethylenimine (PEI) and [ 64 Cu]‐loaded magnetic nanoparticles have been reported for white blood cell (WBC) labelling . [ 64 Cu]‐PTSM proved to be superior to [ 18 F]‐fluorodeoxyglucose‐labelled leukocytes with a higher and more reproducible labelling efficiency .…”

Section: Introductionmentioning

confidence: 99%

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A new technique for the radiolabelling of mixed leukocytes with zirconium‐89 for inflammation imaging with positron emission tomography

Fairclough¹,

Prenant²,

Ellis

et al. 2016

Labelled Comp Radiopharmac

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Mixed leukocyte (white blood cells [WBCs]) trafficking using positron emission tomography (PET) is receiving growing interest to diagnose and monitor inflammatory conditions. PET, a high sensitivity molecular imaging technique, allows precise quantification of the signal produced from radiolabelled moieties. We have evaluated a new method for radiolabelling WBCs with either zirconium‐89 (89Zr) or copper‐64 (64Cu) for PET imaging. Chitosan nanoparticles (CNs) were produced by a process of ionotropic gelation and used to deliver radiometals into WBCs. Experiments were carried out using mixed WBCs freshly isolated from whole human blood. WBCs radiolabelling efficiency was higher with [89Zr]‐loaded CN (76.8 ± 9.6% (n = 12)) than with [64Cu]‐loaded CN (26.3 ± 7.0 % (n = 7)). [89Zr]‐WBCs showed an initial loss of 28.4 ± 5.8% (n = 2) of the radioactivity after 2 h. This loss was then followed by a plateau as 89Zr remains stable in the cells. [64Cu]‐WBCs showed a loss of 85 ± 6% (n = 3) of the radioactivity after 1 h, which increased to 96 ± 6% (n = 3) loss after 3 h. WBC labelling with [89Zr]‐loaded CN showed a fast kinetic of leukocyte association, high labelling efficiency and a relatively good retention of the radioactivity. This method using 89Zr has a potential application for PET imaging of inflammation.

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Radiolabeling of Nanoparticles

Zhang

2016

Toxicology of Nanomaterials

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Labelling of Granulocytes by Phagocytic Engulfment with 64Cu-Labelled Chitosan-Coated Magnetic Nanoparticles

Cited by 17 publications

References 23 publications

Nanoparticles for Imaging: Top or Flop?

Nanoparticles for Imaging: Top or Flop?

A new technique for the radiolabelling of mixed leukocytes with zirconium‐89 for inflammation imaging with positron emission tomography

Radiolabeling of Nanoparticles

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