Labelling of antibiotic peptide - edeine B with 14C

Abstract

Labelling of antibiotio peptideedeine B with 14C Janina 2upahaka, Jadwiga Uqsowioz, K 8 s i m i e r s Daiggielmdcix Institute of Nuolear Researoh, swierk -Otwook, Poland Summary Edeine B with guanyl group labelled with 14C y . 8 obtained by treating edeine A with a 6-fold exoeae of O-a~ethylisourea-~~C. The radioohemioal yield m a loll$. The produot was purified on a oolumn, filled with Sephadex LH 20 and by preparative paper ohromatography. The radioohemioal purity of the obtained preparation wms 97.75. Edeine B is one of the oomponents of the edeine oopplex, oompounds of the antibiotio peptide s o u p /1,2/. These oompounds exhibit two-direotional aotiont baoteriostatio on one hand, stimulating in FWA syntheaia and inhibitin6 in DNA 8ynthesia /3-5/, on the other hand. Owing to its biologioal aotivity edeine B ia uaed in the atudies of ba8io life prooe8aea. Labelling it with 14C make8 possible applioation of isotope teohaiquets. Tarohomin Pharmaceutioal Vorks mOolftaw , Varasv, Poland 79 0362-480 3/76/00 15-00 79S0 1 .00 Q1978 by John Wiley & Sons Ltd.