Labelled StealthR liposomes in experimental infection

Oyen, W.J.G.; Boerman, Otto C.; Storm, Gert; Bloois, Louis van; Koenders, E.B.; Crommelin, Daan J.A.; Meer, J.W.M. van der; Corstens, F.H.M.

doi:10.1097/00006231-199609000-00004

Cited by 31 publications

(9 citation statements)

References 9 publications

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“…The physicochemical characteristics of these PEGylated liposomes do not influence the degree of localization of liposomes at the infection site (20). This property is exploited in medical diagnosis, and 99m technetium-labelled PEG liposomes may be an attractive alternative for labelled leukocytes to image inflammatory bowel disease, because they can be prepared off the shelf and no handling of blood is required (21)(22)(23).…”

Section: Effect Of In¯ammation Due To the Septic Shock (Endotoxin Chamentioning

confidence: 99%

In vivo behaviour of long‐circulating liposomes in blood vessels in hamster inflammation and septic shock models—use of intravital fluorescence microscopy

Devoisselle¹,

Bégu²,

Tourné-Péteilh³

et al. 2001

Luminescence

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This study aimed to observe liposome uptake by leukocytes in vivo. The study was performed on skin by using a dorsal skin-fold chamber implanted in golden hamsters using intravital microscopy. 5 and 6-CF-encapsulated polyethylene glycolated liposomes were injected intravenously. The skin microcirculation was observed with an intravital Eclipse E800 Nikon microscope (using x40, x80 magnification) fitted with a Xenon light source and an epifluorescence assembly (excitation, 470 nm, FWHM 40 nm; emission, 540 nm, FWHM 40 nm). An ultra-high sensitivity videocamera mounted on the microscope projected the image onto a monitor, and the images (720 x 576 pixels) were recorded for playback analysis with a digital video cassette recorder. An acute inflammatory response was obtained by removing one complete layer of skin and the underlying fascia and avascular tissue on the opposing side of the flap corresponding to an area equivalent to the window aperture. Using this model and set-up, leukocyte rolling and adhesion were easily observed and the entry of PEGylated liposomes into hamster blood leukocytes was studied for a period of 6 h. PEGylated liposomes were clearly identified alone inside the blood flow and inside the leukocytes as soon as the inflammatory reaction appeared. This study shows for the first time that blood leukocytes in their natural milieu of whole blood are capable of interacting with, and taking up, liposomes. This observation is in accordance with previous in vitro studies.

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Section: Effect Of In¯ammation Due To the Septic Shock (Endotoxin Chamentioning

confidence: 99%

In vivo behaviour of long‐circulating liposomes in blood vessels in hamster inflammation and septic shock models—use of intravital fluorescence microscopy

Devoisselle¹,

Bégu²,

Tourné-Péteilh³

et al. 2001

Luminescence

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“…Liposomes: Liposomes, lipid vesicles that are formed as a result of thermodynamic arrangements where an aqueous core is surrounded by two phospholipid layers, have been investigated for infection imaging [126,127,130]. Their size varies between 0.1 to 0.5 µm.…”

Section: Radiolabelledmentioning

confidence: 99%

“…Liposomes are removed from the circulation by the mononuclear phagocytic system (MPS) by being recognized as foreign particles. Also the increased clearance from the blood did not result in a major improvement in targeting as the radiolabel stayed in the body after being eliminated from the blood [137]. Reduction of the size, minimization of charge and inclusion of cholesterol have partially helped to minimize the recognition of the liposomes by the MPS.…”

Section: Radiolabelledmentioning

confidence: 99%

“…The short circulation time resulting from their rapid uptake by MPS has been an obstacle in their clinical applications [131]. In various infection models, radiolabeled PEG-liposomes performed equal to or even better than conventional RPs such as Ga-67, radiolabelled WBCs and Tc-99m-HIG [126]. Recently, the development of sterically stabilized liposomes where the lipid bilayer contains hydrophilic components such as polyethylene glycol (PEG) [132,133], offered steric hindrance to plasma opsonin adsorbtion and consequently the liposomes are protected from recognition by the MPS [134,135].…”

Section: Radiolabelledmentioning

confidence: 99%

“…Typical nanoparticles include 99m Tc-albumin nanocolloid [27,[121][122][123][124][125] and liposomes [126,127]. Due to their small size nanoparticles remain in circulation and make contact with inflammatory/infected cells for relatively longer periods of time.…”

Section: Radiolabelled Nanocolloids and Liposomesmentioning

confidence: 99%

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Radiopharmaceuticals for the Visualization of Infectious and Inflammatory Lesions

Ercan¹,

Kostakoğlu²

2000

CPD

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Many radiopharmaceuticals have been introduced for the scintigraphic demonstration of infectious and inflammatory lesions and some of them are currently in clinical use. They can be classified into two major categories according to their specificity. Specific radiopharmaceuticals include in vitro labeled leukocytes, radio-labelled monoclonal antibodies, and receptor specific small proteins and peptides. Nonspecific radiopharmaceuticals include radiolabelled nanocolloids, liposomes, macromolecules such as human immunoglobulin, dextran and human serum albumin, various small molecules and ions. In this review article radiopharmaceuticals in their respective groups are discussed as to the efficacy, and other parameters such as the physical characteristics of the radionuclides used, radiochemistry involved, availability, cost and biodistribution, emphasizing recent developments.

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Use of Radiolabeled Liposomes for PEG-Liposome-Based Drug Targeting and Diagnostic Imaging Applications

Phillips

1998

Targeting of Drugs 6

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Labelled StealthR liposomes in experimental infection

Cited by 31 publications

References 9 publications

In vivo behaviour of long‐circulating liposomes in blood vessels in hamster inflammation and septic shock models—use of intravital fluorescence microscopy

In vivo behaviour of long‐circulating liposomes in blood vessels in hamster inflammation and septic shock models—use of intravital fluorescence microscopy

Radiopharmaceuticals for the Visualization of Infectious and Inflammatory Lesions

Use of Radiolabeled Liposomes for PEG-Liposome-Based Drug Targeting and Diagnostic Imaging Applications

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