Labeling quality and molecular characterization studies of products containing <i>Lactobacillus</i> spp. strains.

Blandino, G; Fazio, Davide; Petronio, Giulio Petronio; Inturri, Rosanna; Tempera, G; Furneri, Pio Maria

doi:10.1177/0394632015600534

Cited by 10 publications

(10 citation statements)

References 22 publications

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“…They also confirmed that the 16S rRNA gene sequence was inadequate for distinguishing between species in the L. casei group. The misidentification of a commercial L. casei strain (DG, CNCM I-1572) found in an Italian pharmaceutical product was also reported by Blandino et al (2016) , who successfully used multiplex PCR based on tuf gene amplification ( Blandino et al, 2016 ).…”

Section: Discussionmentioning

confidence: 72%

Evaluation of Dietary Supplements Containing Viable Bacteria by Cultivation/MALDI-TOF Mass Spectrometry and PCR Identification

Lorbeg

Golob

Kramer³

et al. 2021

Front. Microbiol.

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The insufficient quality of products containing beneficial live bacteria in terms of content and viability of labelled microorganisms is an often-reported problem. The aim of this work was to evaluate the quality of dietary supplements containing viable bacteria available in Slovenian pharmacies using plate counting, matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and species- or subspecies-specific PCR with DNA isolated from consortia of viable bacteria, from individual isolates, or directly from the products. Twelve percent of the products (3 of 26) contained insufficient numbers of viable bacteria. Eighty-three of the labelled species (111 in total) were confirmed by PCR with DNA from the product; 74% of these were confirmed by PCR with DNA from viable consortium, and 65% of these were confirmed by MALDI-TOF MS analysis of colonies. Certain species in multi-strain products were confirmed by PCR with DNA from viable consortia but not by MALDI-TOF MS, suggesting that the number of isolates examined (three per labelled strain) was too low. With the exception of Lacticaseibacillus casei and closely related species (Lacticaseibacillus rhamnosus and Lacticaseibacillus zeae), PCR and MALDI-TOF identification results agreed for 99% of the isolates examined, although several MALDI-TOF results had lower score values (1.700–1.999), indicating that the species identification was not reliable. The species L. zeae, which appeared in 20 matches of the Biotyper analysis, was identified as L. rhamnosus by PCR. The MALDI-TOF MS analysis was also unsuccessful in detecting Lactobacillus acidophilus La-5 and Bacillus coagulans due to missing peaks and unreliable identification, respectively. Mislabelling was detected by both methods for two putative L. casei strains that turned out to belong to the species Lacticaseibacillus paracasei. PCR remains more successful in subspecies-level identification as long as the database of MALDI-TOF MS spectra is not expanded by building in-house databases. The lack of positive PCR results with viable consortia or colonies, but positive PCR results with DNA isolated directly from the products observed in 10% (11/112) of the labelled strains, suggests the presence of non-culturable bacteria in the products. MALDI-TOF MS is a faster and simpler alternative to PCR identification, provided that a sufficient number of colonies are examined. Generation of in-house library may further improve the identification accuracy at the species and sub-species level.

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Section: Discussionmentioning

confidence: 72%

Evaluation of Dietary Supplements Containing Viable Bacteria by Cultivation/MALDI-TOF Mass Spectrometry and PCR Identification

Lorbeg

Golob

Kramer³

et al. 2021

Front. Microbiol.

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“…The authors found that all products contained L. crispatus by culture-independent methods, but only six formulations carried living cells (Di Pierro et al, 2019). The identification of species declared to be contained in five Italian oral products revealed concordant microbial composition to the label claims for four formulations (Blandino et al, 2016). Identification of species contained in 13 French functional foods by MALDI-TOF MS, PCR and sequencing of tuf and 16S rRNA genes revealed different species than those stated for two products (Angelakis et al, 2011).…”

Section: Microbial Compositionmentioning

confidence: 95%

“…An incongruent microbial amount was found in 5 among 10 investigated European products ( Coeuret et al, 2004 ). Thirty among 46 analyzed Italian products contained the amount of living microbes declared on the labels ( Aureli et al, 2010 ; Blandino et al, 2016 ). Among 24 Italian products tested by Toscano et al (2013) , 10 were found to contain an incorrect amount of cells.…”

Section: Compositional Qualitymentioning

confidence: 99%

Spotlight on the Compositional Quality of Probiotic Formulations Marketed Worldwide

et al. 2021

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On the worldwide market, a great number of probiotic formulations are available to consumers as drugs, dietary supplements, and functional foods. For exerting their beneficial effects on host health, these preparations should contain a sufficient amount of the indicated living microbes and be pathogen-free to be safe. Therefore, the contained microbial species and their amount until product expiry are required to be accurately reported on the labels. While commercial formulations licensed as drugs are subjected to rigorous quality controls, less stringent regulations are generally applied to preparations categorized as dietary supplements and functional foods. Many reports indicated that the content of several probiotic formulations does not always correspond to the label claims in terms of microbial identification, number of living organisms, and purity, highlighting the requirement for more stringent quality controls by manufacturers. The main focus of this review is to provide an in-depth overview of the microbiological quality of probiotic formulations commercialized worldwide. Many incongruences in the compositional quality of some probiotic formulations available on the worldwide market were highlighted. Even if manufacturers carry at least some of the responsibility for these inconsistencies, studies that analyze probiotic products should be conducted following recommended and up-to-date methodologies.

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“…This was, for instance, an issue in the context of probiotic supplements where it was found that ingredient lists occasionally do not correspond to the microorganisms present in the products (e.g. Lewis et al 2016, Blandino et al 2016.…”

Section: Taxonomic Identification Of Microorganisms Usedmentioning

confidence: 99%

Status of microbial based cleaning products in statutory regulations and ecolabelling in Europe, the USA, and Canada

Spök

Arvanitakis

McClung

2018

Food and Chemical Toxicology

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Cleaning products containing living microorganisms as active ingredients are increasingly being used in household, professional and industrial cleaning applications. Microorganisms can degrade soiling associated with dirt, food residues, and grease by enzymatic and metabolic action and out-compete microorganisms associated with odor problems. Their potential for odor control seems to result in a competitive advantage over conventional chemically-based cleaning products. Moreover, producers of microbial-based cleaning products (MBCPs) claim that their products are less harmful to the environment. These promising prospects have triggered interest from consumer and environmental organizations, professional users, and regulators in understanding if there are also possible negative health and environmental impacts which require attention and how the safety of these products is ensured. Unfortunately, there is little information on these issues in the public domain. Moreover, regulatory oversight in Europe is essentially limited to pathogenic properties in the context of worker protection. Canada, in contrast, has a regulatory framework in place to assess risks to human health and the environment from the manufacture, import and/or use of new microorganisms contained in MBCPs. In the absence of mandatory standards, safety assessment and hygienic practices seem to vary considerably across companies. Recently developed ecolabelling standards are - for the time being - the only option for transparent compliance to minimum standards in terms of safety as well as assessments of manufacturer information by third parties. These standards highlight in particular the need for precise taxonomic information for assessing the pathogenic properties and the need to ensure the absence of potentially harmful microorganisms as contaminants. Ecolabelling standards are, however, voluntary and do not cover all relevant safety issues. In order to develop a more comprehensive set of mandatory standards for health and safety, a number of areas would benefit from further research (e.g. the role in plant pathogenicity and other environmental properties of the microorganisms used; the relevance of chronic exposure to dusts and aerosols containing vegetative cells and spores; the relevance of strains which belong to species known to include opportunistic pathogens and possible hazards for particularly sensitive risk groups). Improved knowledge in these areas will contribute to a predictable level of product safety.

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Labeling quality and molecular characterization studies of products containing Lactobacillus spp. strains.

Cited by 10 publications

References 22 publications

Evaluation of Dietary Supplements Containing Viable Bacteria by Cultivation/MALDI-TOF Mass Spectrometry and PCR Identification

Evaluation of Dietary Supplements Containing Viable Bacteria by Cultivation/MALDI-TOF Mass Spectrometry and PCR Identification

Spotlight on the Compositional Quality of Probiotic Formulations Marketed Worldwide

Status of microbial based cleaning products in statutory regulations and ecolabelling in Europe, the USA, and Canada

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