Labeling of neuronal differentiation and neuron cells with biocompatible fluorescent nanodiamonds

Hsu, Tzu Chia; Liu, Kuang Kai; Chang, Huan‐Cheng; Hwang, Eric; Ji, Chao

doi:10.1038/srep05004

Cited by 77 publications

(61 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A practical function of FNDs is their use in tracking stem cell. FNDs have been successfully used to monitor stem cell differentiation, without any observed changes in resulting cell morphology or metabolism [27]. There are additional in vivo studies reporting the use of FNDs for monitoring the efficacy of stem cell therapy [28,29].…”

Section: Other Emerging Biomedical Applications Of Ndsmentioning

confidence: 99%

Adoption of nanodiamonds as biomedical materials for bone repair

et al. 2017

View full text Add to dashboard Cite

Keywords: gene/drug delivery • nanodiamonds • therapeutics Nanodiamonds (NDs) are an emerging class of biologically compatible carbon-based nanomaterials that possess a set of unique properties essential for the design of innovative therapies in the fields of drug delivery, tissue engineering and bioimaging [1]. For instance, the high surface area along with the tunable surface chemistry enables the physical adsorption or covalent conjugation of a variety of therapeutic molecules, such as drugs, peptides and growth factors [2][3][4][5]. Additionally, NDs' surface can be modified with targeting biological signals to achieve a selective cellular internalization of chemotherapeutic agents as well as genetic materials. Aside from their role as carriers in drug and gene delivery, NDs have been widely explored as nanofillers to improve the physical and mechanical properties of both natural and synthetic scaffolds [6,7]. Specifically, NDs have found application in the field of bone tissue engineering as reinforcing nanomaterial to design nanocomposite systems necessary to promote bone regeneration. This editorial emphasizes on the current state-of-the-art research using NDs in the field of bone repair, with a focus on the recent breakthroughs such as precise immobilization of growth factors and peptides. Biomolecule immobilization via surface functionalization of NDsNDs are carbon-based nanomaterials, which possess a set of unique properties that enables their use in a variety of applications including drug delivery, tissue engineering and bioimaging. This advantage has encouraged scientists to investigate NDs for a broad range of applications [8] in regenerative medicine with a primary focus on bone tissue engineering. NDs possess versatile surface functionalities and they can be loaded with virtually any type of biomolecule, thereby enabling their use as delivery vehicles in bone-healing applications [9]. The presence of surface functional groups can efficiently control the interfacial reactions between the nanoparticle and the active biomolecule, eventually maximizing the therapeutic efficiency of the delivered biomaterial. The initial functional groups present on the surface of NDs, following the formation of the nanoparticle, vary according to the selected synthesis and purification methods. For example, hydrogen-assisted chemical vapor deposition generates NDs bearing hydrogen-terminated surfaces. On the other hand, methods, such as detonation synthesis, introduce a highly diverse surface chemistry, with functional groups such as hydroxyl, carbonyl, ether and carboxyl groups. It is essential to maintain a similar functionality throughout the entire surface to enable further surface reactions. Thus, chemical treatment of the NDs is often required to create homogenized reactive surfaces. Among the possible alternatives, carboxylation, hydroxylation or hydrogenation are the commonly investigated routes to generate -COOH and -OH groups on the surface. Due to the presence of these diverse functionalities on the surfac...

show abstract

Section: Other Emerging Biomedical Applications Of Ndsmentioning

confidence: 99%

Adoption of nanodiamonds as biomedical materials for bone repair

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In addition, the surface passivation of diamond nanoparticles is well developed [12][13][14][15][16] enabling selective attachment of nanodiamonds to specific organelles of the cell, and therefore, in vivo measurement of their temperature response under various conditions.…”

mentioning

confidence: 99%

Germanium-Vacancy Color Center in Diamond as a Temperature Sensor

et al. 2018

View full text Add to dashboard Cite

show abstract

“…There are numerous approaches reported in the literature on neuronal differentiation of NT2 cells in aggregate or suspension culture, as well as in monolayer cultures [21,[25][26][27][28][29][30][31][32]. These protocols Sepideh Abolpour Mofrad performed the present work in fulfillment of the requirements for obtaining the degree Dr. rer.…”

Section: Spoiled For Choice -Which Protocol To Choose For a Cell-basementioning

confidence: 99%

On the Generation of Efficient Methodologies for Cell-Based Toxicity Screening by Comparative Protocol Analysis and Optimization

Mofrad¹,

Kuenzel²,

Friedrich³

et al. 2017

Single Cell Biol

View full text Add to dashboard Cite

In cell biology in general and in high-throughput cell-based screening approaches in particular -e.g. for in vitrobased toxicity assessment -various methodologies or protocols are reported in the literature. In the case that a cell-based screening assay, for toxicity evaluation or for assessing another biological question, is to be established for the first time in a research lab, the researcher has to select a number of different protocols from the literature and to create an optimized protocol for the intended use. This is typically required, because optimized protocols, generated following comparative protocol analysis and optimization, are mostly rarely available. In another study, which we refer to as a showcase, we conducted a comparative analysis of three different protocols for neuronal NT2 cell differentiation, available in the literature. From this comparison, we generated an improved and optimized method, allowing for neuronal NT2 differentiation in monolayer cultures with high yield of NT2-N cells, allowing for systematic in vitro-based primary screening for developmental toxicants and neuro-toxicants at different stages of maturation. In this commentary, to prevent the same experiments from being repeatedly conducted in different labs around the world and at different times over and over again, we suggest generation of advanced and efficient methods by comparative protocol analysis and optimization, for application in a variety of research fields, related to cell and single cell biology. Keywords:In vitro DNT testing; NT2 human pluripotent stem cells; Protocol optimization; High-throughput screening Showcase: In vitro-Based DNT TestingEveryday products such as cosmetics, pharmaceuticals, textiles, detergents, plastic or pesticides, contain organic and inorganic chemical substances, that are potentially harmful to humans and the ecosystems. The number and volume of worldwide traded and registered chemicals has dramatically increased in recent years. Simultaneously, the incidence of neurological diseases, including mental retardation and autism, as well as developmental and learning disorders or hyperactivity disorder and attention deficit, has also increased [1][2][3]. The developing central nervous system (CNS) is particularly susceptible to damage by chemicals and therefore, assessment of the chemicals surrounding us on a daily basis for adverse effects on the maturing CNS -also referred to as developmental neurotoxicity (DNT) -is critical for human health and wealth [4][5][6]. Currently, as of January 2017, the CAS Registry (Chemical Abstracts Service), the world's largest database of chemical substances, lists more than 126 million unique organic and inorganic chemicals. Of these chemicals, only very few representatives have been evaluated for DNT in recent years [7,8]. The reason for this is possibly because existing guidelines for toxicity evaluation mostly involve animal experiments that are expensive, low in throughput, of poor predictive quality, often not reproducible and, because t...

show abstract

Labeling of neuronal differentiation and neuron cells with biocompatible fluorescent nanodiamonds

Cited by 77 publications

References 50 publications

Adoption of nanodiamonds as biomedical materials for bone repair

Adoption of nanodiamonds as biomedical materials for bone repair

Germanium-Vacancy Color Center in Diamond as a Temperature Sensor

On the Generation of Efficient Methodologies for Cell-Based Toxicity Screening by Comparative Protocol Analysis and Optimization

Contact Info

Product

Resources

About