Labeling of cancer cells with magnetic nanoparticles for magnetic resonance imaging

Abstract

These results suggest that PLL-coated γFe2 O3 nanoparticles can be used as an MRI contrast agent for long-term studies of cell migration. Magn Reson Med 71:1896-1905, 2014. © 2013 Wiley Periodicals, Inc.

Nanoparticles and clinically applicable cell tracking

“…A similar effect of intracellular clustering on relaxation properties was found for iron oxide-based nanoparticles. 114 As a negative effect of larger particle size, the authors found a higher toxicity level, reflected in reduced cell viability, for the larger particles than for the smaller particles. 35 The maximal tolerated dose for labelling, that is without affecting cell viability, was approximately two-fold higher for the smaller particles.…”

“…115 However, several studies did show negative effects on cellular function following labelling with iron oxide particles at lower doses, including negative effects on multilineage differentiation capacity, 116 migratory capacity, 104 altered cytokine production 117 or cell survival. 118 In a review by Singh et al, 114 the various mechanisms by which iron oxide agents can exert adverse effects on cell function have been discussed, and include effects on membrane integrity, mitochondrial function, generation of reactive oxygen species and DNA damage. In many cases, the toxic effects are dose dependent and related to specific nanoparticle composition aspects.…”

“…78,99,121,122 Because of these findings there is a continued effort in trying to generate more stable particles and/or particles with an improved balance between core composition and coating. 77,114,[123][124][125] In our group, we made the observation that for a liposomal encapsulated gadolinium particle, adverse effects on cellular functionality was more directly associated with the liposomal capsule than with the chelated gadolinium molecules. 31 The maximal tolerated dose of the cell label was identical for Gdencapsulated liposomes as for empty liposomes.…”

Preparation of Lysine-Coated Magnetic Fe2O3Nanoparticles and Influence on Viability of A549 Lung Cancer Cells

“…Magnetic nanoparticles (MNPs), which have unique properties of superparamagnetism, alternating current magnetic heating, magnetic quantum tunnel effect and good biocompatibility, is widely applied in the field of biomedical domain such as magnetic resonance imaging (MRI) contrast agent, immunoassay, magnetic heat treatment, drug release and cell sorting (Fang et al, 2012;Sterenczak et al, 2012;Rosen et al, 2012;Barick et al, 2014;Bogart et al, 2014;Singh et al, 2014;Wabler et al, 2014;Weis et al, 2014).…”

“…MNPs shows great potential in MRI contrast agent, however, there is a limitation in application of MNPs achieving cell imaging in the current study. For example, efficient uptake nanoparticles, effective gathering in the lesions, imaging signal amplification and the improvement of imaging quality are needed to be further studied (Bakhru et al, 2012;Laesen et al, 2012;Weis et al, 2014).…”