Labeling knife cuts: A new method for revealing the functional anatomy of the CNS demonstrated on the noradrenergic dorsal bundle

Scouten, Charles W.; Harley, Carolyn W.; Malsbury, Charles W.

doi:10.1016/0361-9230(82)90050-8

Cited by 12 publications

(4 citation statements)

References 10 publications

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“…The second experiment was concerned with lateral MPOA afferents that may be involved in maternal behavior. Females received MPOA lateral cuts with a horseradish peroxidase (HRP)–coated wire knife (the labeling knife cut procedure: Numan, Morrell, & Pfaff, 1985; Scouten, Harley, & Malsbury, 1982). The HRP would be taken up by cut axons and transported to their cell bodies, thereby serving as a marker for neuronal groups severed by the cuts.…”

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confidence: 99%

Dorsolateral connections of the medial preoptic area and maternal behavior in rats.

McSparren¹,

Numan²

1990

Behavioral Neuroscience

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The lateral connections of the medial preoptic area (MPOA) are essential for maternal behavior in rats. The purpose of this study was to more exactly specify the nature of this pathway. Experiment 1 found that knife cuts that severed the dorsolateral connections of the MPOA were as effective as complete cuts in disrupting maternal behavior, whereas knife cuts that severed the ventrolateral MPOA connections were ineffective. These results suggest that MPOA efferents and afferents critical for maternal behavior leave or enter the MPOA dorsolaterally. Experiment 2 located possible sources of critical afferent input. Lactating rats received MPOA lateral cuts with a horseradish peroxidase (HRP)-coated wire knife. Full lateral cuts and dorsolateral cuts disrupted maternal behavior and labeled more cells with HRP in the nucleus of the solitary tract and the locus coeruleus than did ventrolateral cuts, which did not disrupt maternal behavior.

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confidence: 99%

Dorsolateral connections of the medial preoptic area and maternal behavior in rats.

McSparren¹,

Numan²

1990

Behavioral Neuroscience

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“…Knife cut ventral to the striatum. A Scouten wire knife (Scouten et al, 1982) (David Kopf Instruments) was inserted vertically at the intraaural coordinates A 8.7, L 2.9, V -7.7. A horizontal cut, ventral to the striaturn, was performed by drawing out the knife blade from its hose in the parasagittal direction and by turning it once-ventrally to the striatum-for 180" into the opposite parasagittal direction.…”

Section: R Widmann Et Almentioning

confidence: 99%

Evidence for Somatostatin‐Containing Fibers Projecting from the Pallidal Complex to the Striatum of the Rat

Widmann

Mensdorff-Pouilly

Pfaller

et al. 1987

Journal of Neurochemistry

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The origin of afferent somatostatin-containing fibers terminating in medial and ventral parts of the striatum has been investigated by performing various neurochemical and surgical lesions in the rat. Lesions of the anterior hypothalamus, amygdala, and the hippocampal commissure as well as lesions with 6-hydroxydopamine and 5,7-dihydroxytryptamine failed to decrease striatal somatostatin levels. However, thermal coagulation of the globus pallidus or knife-cut lesions performed ventrally to the striatum resulted in significant decreases in striatal somatostatin content. Analysis of the topographical distribution of somatostatin within the striatum after thermal lesions of the globus pallidus as well as after kainic acid-induced seizures revealed a preferential loss of the peptide in medial and ventral portions of the striatum, the site of terminating afferent somatostatin nerve fibers. The data suggest that the striatal afferent somatostatin-containing neurons may originate in the area of the globus pallidus.

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“…The LSv, caudal BSTm, and MA–AHi were of particular interest because knife cuts that eliminate mating in male gerbils (i.e., parasagittal cuts lateral to the medial SDA) sever axons of neurons in these areas, but knife cuts that do not affect mating (i.e., cuts lateral to the lateral SDA) do not (Yahr & Jacobsen, 1994). This was shown by making cuts lateral to the medial or lateral SDA with a knife coated with horseradish peroxidase, a retrograde tracer that readily enters cut axons (Mesulam, 1982; Scouten, Harley, & Malsbury, 1982).…”

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confidence: 99%

Telencephalic connections of the sexually dimorphic area of the gerbil hypothalamus that influence male sexual behavior.

Sayag¹,

Hoffman²,

Yahr³

1994

Behavioral Neuroscience

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The sexually dimorphic area (SDA) of the gerbil hypothalamus is essential for mating in male gerbils. To determine if it affects mating through its connections with the ventral part of the lateral septal nucleus (LSv), the caudal part of the medial bed nucleus of the stria terminalis (caudal BSTm), or the medial amygdala-amygdalohippocampal area (MA-AHi), these connections were severed. Unilateral cell-body lesions were made in the SDA and in the contralateral LSv, caudal BSTm, or MA-AHi. Controls received the same lesions ipsilaterally. Other gerbils received lesions in the caudal BSTm and contra- or ipsilateral MA-AHi. Only contralateral lesions of the SDA and caudal BSTm severely impaired mating. Because contralateral lesions of the SDA and MA-AHi, or BSTm and MA-AHi, did not mimic this effect, the BSTm neurons that are needed for male sexual behavior through their connections with the SDA do not simply relay information to or from the MA-AHi.

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Labeling knife cuts: A new method for revealing the functional anatomy of the CNS demonstrated on the noradrenergic dorsal bundle

Cited by 12 publications

References 10 publications

Dorsolateral connections of the medial preoptic area and maternal behavior in rats.

Dorsolateral connections of the medial preoptic area and maternal behavior in rats.

Evidence for Somatostatin‐Containing Fibers Projecting from the Pallidal Complex to the Striatum of the Rat

Telencephalic connections of the sexually dimorphic area of the gerbil hypothalamus that influence male sexual behavior.

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