Labeling and Intracellular Tracking of Functionally Active Plasmid DNA with Semiconductor Quantum Dots

Srinivasan, Charudharshini; Lee, Jeunghoon; Papadimitrakopoulos, Fotios; Silbart, Lawrence K.; Zhao, Minhua; Burgess, Diane J.

doi:10.1016/j.ymthe.2006.03.010

Cited by 120 publications

(100 citation statements)

References 45 publications

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“…In the self-assembly of nanoparticles, surface properties determining the interactions between particles play a fundamental role (Niemeyer 2001;Shenhar et al 2005;Srinivasan et al 2006;Ofir et al 2008;Talapin 2008;Lim & Zhong 2009). Finally, owing to the extremely high surface-to-volume ratio of nanoparticles, they may be ideally suited for catalytic purposes, as recently summarized in a review (Narayanan & El-Sayed 2008).…”

Section: Applications Outlookmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,396

1,092

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Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the form of fluorescence (semiconductor quantum dots, e.g. CdSe or CdTe) or phosphorescence (doped oxide materials, e.g. Y 2 O 3 ), or magnetic moment (e.g. iron oxide or cobalt nanoparticles). Prerequisite for every possible application is the proper surface functionalization of such nanoparticles, which determines their interaction with the environment. These interactions ultimately affect the colloidal stability of the particles, and may yield to a controlled assembly or to the delivery of nanoparticles to a target, e.g. by appropriate functional molecules on the particle surface. This work aims to review different strategies of surface modification and functionalization of inorganic colloidal nanoparticles with a special focus on the material systems gold and semiconductor nanoparticles, such as CdSe/ZnS. However, the discussed strategies are often of general nature and apply in the same way to nanoparticles of other materials.

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Section: Applications Outlookmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,396

1,092

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“…Another bifunctional linker called succinimidyl 3-(2-pyridyldithio)propionate (SPDP) can also be used to functionalize amine terminating DNA to QDs [83]. This linker has an NHS group on one end and a cleavable pyridyl disulfide on the other.…”

Section: Amine To Thiol Reactive Linkersmentioning

confidence: 99%

“…Covalent conjugation of biomolecules on the surface of QDs is by far the most popular method to prepare rsfs.royalsocietypublishing.org Interface Focus 6: 20160064 [80 -82] QDs with amines thiol modified [83] QDs with thiols amine modified cyclooctyne [84] QDs with azide -reactants are not prone to hydrolysis -loss of QY [85] rsfs.royalsocietypublishing.org Interface Focus 6: 20160064…”

Section: Click Chemistrymentioning

confidence: 99%

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Quantum dots–DNA bioconjugates: synthesis to applications

et al. 2016

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Semiconductor nanoparticles particularly quantum dots (QDs) are interesting alternatives to organic fluorophores for a range of applications such as biosensing, imaging and therapeutics. Addition of a programmable scaffold such as DNA to QDs further expands the scope and applicability of these hybrid nanomaterials in biology. In this review, the most important stages of preparation of QD-DNA conjugates for specific applications in biology are discussed. Special emphasis is laid on (i) the most successful strategies to disperse QDs in aqueous media, (ii) the range of different conjugation with detailed discussion about specific merits and demerits in each case, and (iii) typical applications of these conjugates in the context of biology.

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“…Some challenging reports on Quantum Dots-mediated gene delivery have been studied, for example the delivery of plasmid DNA [12] into the animal cells, and stable transfection and high expression efficiency were obtained, which have laid a good foundation for the application of QDs in plant genetic transformation. The ability of carbon nanotubes to penetrate intact plant cell wall and cell membrane has already been reported.…”

Section: Transgene Nanovehiclesmentioning

confidence: 99%