Labeled Staphylococcai Protein A as an Immunological Probe in the Analysis of Cell Surface Markers

Dorval, G.; Welsh, Ken I.; Wigzell, Hans

doi:10.1111/j.1365-3083.1974.tb01273.x

Cited by 95 publications

(27 citation statements)

References 11 publications

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“…The percentage of killed cells in the latter combination was 5-20%. Antigen-binding anti-H-2 antibodies were detected as described previously (24).…”

Section: Methodsmentioning

confidence: 99%

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Rubin¹,

Hertel-Wulff²,

Kimura³

1979

The Journal of Experimental Medicine

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The present study describes the qualitative reactions of a xenogeneic anti-idiotype (Id) antiserum produced in a mouse-gamma-globulin-tolerant rabbit (5,936) against B6 anti-CBA IgG antibodies. The results showed that such an anti-Id antiserum reacts specifically against anti-H-2k antibodies and against H-2k alloantigen-activated T cells from the following pairs of congenic mice: B10 (H-2b) and B10.D2 (H-2d); and A.BY (H-2b) and A.SW (H-2s), but not against C3H.SW (H-2b) and C3H.OH (H-2o); and BALB/b (H-2b) and BALB/c (H-2d). CB 20 (BALB/c mice with the Ig-1b allotype) anti-CBA T blasts also express idiotypic determinants that react with rabbit 5,936 antiserum. Thus, positive reactions are obtained between rabbit 5,936 anti-Id antiserum and anti-H-2k IgG preparations and T blasts from mice carrying the Ig-1b or Ig-1e allotype, but not from mice carrying the Ig-1a allotype. These reactions are qualitatively independent of the H-2 genotype of the Id-producing mice. Such a finding strongly suggests that the Id-bearing receptor molecules on mouse T cells are coded for by genes that are associated with the Ig heavy-chain-linkage group and not to the mouse histocompatibility complex. Furthermore, the anti-Id antibodies studied react preferentially against anti-H-2k antibodies or T cells with specificity toward the IAk-region-associated serological specificities. Thus, genes associated with the Ig heavy-chain-linkage group seem to be structural genes for at least T-cell receptors with specificity for IA-region-coded membrane antigens.

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“…The percentage of killed cells in the latter combination was 5-20%. Antigen-binding anti-H-2 antibodies were detected as described previously (24).…”

Section: Methodsmentioning

confidence: 99%

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Rubin¹,

Hertel-Wulff²,

Kimura³

1979

The Journal of Experimental Medicine

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“…Protein A from Staphylococcus aureus is a molecule with specificity for Fc of most mammalian IgG classes (23) . It can be iodinated and used as a sensitive probe for cell-bound IgG molecules (21) . The procedure was identical to the second one described previously for radiolabeled rabbit antirat Ig, and has been described previously in detail (21) .…”

Section: Rat Lymphocyte Preparationsmentioning

confidence: 99%

Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. I. Demonstration of similar or identical idiotypes on IgG molecules and T-cell receptors with specificity for the same alloantigens.

Binz¹,

Wigzell²

1975

The Journal of Experimental Medicine

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335

131

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Antigen-binding receptors on T lymphocytes and IgG antibodies with the same antigen-binding specificity as the T-cell receptors display shared or identical idiotypes. This was shown using a system where adult F1 hybrid rats between two inbred strains were inoculated with T lymphocytes from one parental strain. Such F1 hybrid rats produce antibodies directed against idiotypic determinants present on IgG alloantibodies, produced in the T donor genotype strain and with specificity for the alloantigens of the other parental strain. The idiotypic nature of the F1 antialloantibody serum against the parental alloantibodies was demonstrated both by indirect hemagglutination tests or by gel diffusion using alloantisera with different specificity as targets. Furthermore, the F1 anti-T-lymphocyte sera could be shown to contain antibodies against idiotypic parental T lymphocytes as well. This was shown by the capacity of the antisera, in the presence of complement, to wipe out the relevant parental T-cell reactivity against the other parental strain (as measured in MLC or GVH) whilst leaving the T-lymphocyte reactivity against a third, unrelated allogeneic strain intact. These findings demonstrate that F1 hybrid rats inoculated with parental T lymphocytes make anti-idiotypic antibodies directed against both the T cell receptors and IgG alloantibodies of that parental strain with specificity for alloantigens of the other parental strain. In order to prove identity between the anti-idiotypic antibodies against the B and T-cell antigen-binding molecules the following experiments were carried out; highly purified IgG from relevant alloantibody-containing serum in immunosorbent from could be shown to selectively remove both anti-idiotypic activities from the F1 antiserum. Further more, parental normal T lymphocytes could be shown capable of removing from the anti-idiotypic antisera all those antibodies that would cause agglutination of the relevant alloantibody-coated erythrocytes in the indirect agglutination assay. We would thus conclude that T and B lymphocytes reactive against a given antigenic determinant use receptors with antigen-binding areas coded for by the same variable gene subset(s).

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“…How- ever, when other members of the B7 cross-reacting group were tested in the same assay, no reactivity was seen (Table II). Because the cytotoxicity-negative, absorption-positive phenomenon is well known in HLA serology (24), we tested whether the cytotoxic activity of anti-X for B7 cells could be removed by absorption with either Bw22-or B40-bearing cells (Table IV) (22). It appeared that there was a hierarchy of this availability as follows: B27 > B7 > B40 > Bw22.…”

Section: Resultsmentioning

confidence: 99%

“…'25I-Protein A binding assay (22). Anti-X and normal human IgG were ultracentrifuged immediately before use to remove material >1OS (23).…”

Section: Methodsmentioning

confidence: 99%

Public antigenic determinant on a family of HLA-B molecules.

Schwartz¹,

Luehrman²,

Rodey³

1979

J. Clin. Invest.

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Labeled Staphylococcai Protein A as an Immunological Probe in the Analysis of Cell Surface Markers

Cited by 95 publications

References 11 publications

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. I. Demonstration of similar or identical idiotypes on IgG molecules and T-cell receptors with specificity for the same alloantigens.

Public antigenic determinant on a family of HLA-B molecules.

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