Labeled quantitative mass spectrometry to study the host response during aspergillosis in the common bottlenose dolphin (Tursiops truncatus)

Désoubeaux, Guillaume; Piqueras, Maria del Carmen; Le-Bert, Carolina; Fravel, Vanessa; Clauss, Tonya M.; Delaune, Alexa J.; Daniels, Risa; Jensen, Eric D.; Flower, Jennifer E.; Bossart, Gregory D.; Bhattacharya, Sanjoy K.; Cray, Carolyn

doi:10.1016/j.vetmic.2019.03.030

Cited by 5 publications

(6 citation statements)

References 32 publications

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“…For the species T. truncatus , the focus of the present study, lipidomics characterization was only reported in a study characterizing the Cer and sphingosine content in serum, and it association with dolphin metabolic alteration (Sobolesky, Harrell, et al, 2016b). Moreover, other Omics approaches, like metabolomics or proteomics, have also been used to characterize T. truncatus plasma (Desoubeaux et al, 2019; Miller et al, 2017; Sobolesky, Parry, et al, 2016a). Blood transcriptomics studies are also available (Morey et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

The plasma phospholipidome of Tursiops truncatus: From physiological insight to the design of prospective tools for managed cetacean monitorization

Monteiro

Maciel

Melo

et al. 2021

Lipids

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Plasma biochemical analysis remains one of the established ways of monitoring captive marine mammal health. More recently, complementary plasma lipidomic analysis has proven to be a valid tool in disease diagnosis and prevention, with the potential to validate and complement common biochemical analysis, providing a more integrative approach. In this study, we thoroughly characterized the plasma polar lipid content of Tursiops truncatus, the most common cetacean species held under human care. Our results showed that phosphatidylcholine, lysophosphatidylcholine, and sphingomyelins (CerPCho) are the most represented phospholipid classes in T. truncatus plasma. Palmitic, oleic, and stearic acids are the major fatty acid (FA) present esterified to the plasma polar lipids of this species, although some n-3 species are also remarkably present, namely eicosapentaenoic and docosahexaenoic acids. The polar lipidome identified by HILIC LC-MS allowed identifying 304 different lipid species. These species belong to the phosphatidylcholine (103 lipid species), lysophosphatidylcholine (35), phosphatidylethanolamine (71), lysophosphatidylethanolamine (20), phosphatidylglycerol (13), lysophosphatidylglycerol (5), phosphatidylinositol (15), lysophosphatidylinositol (3), phosphatidylserine (6) lysophosphatidylserine (1), and sphimgomyelin (32) classes. This was the first time that the dolphin plasma phospholipid profile was characterized, providing a knowledge that will be important to further understand lipid metabolism and physiological regulation in small cetaceans. Furthermore, this study proved the practicability of the use of plasma lipid profiling for health assessment in marine mammals under human care.

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Section: Discussionmentioning

confidence: 99%

The plasma phospholipidome of Tursiops truncatus: From physiological insight to the design of prospective tools for managed cetacean monitorization

Monteiro

Maciel

Melo

et al. 2021

Lipids

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“…iTRAQ technology is a convenient proteomic tool, very suitable for in vitro exploratory research . This technique should be considered as a preliminary step before validation of new diagnostic markers . It is a powerful method to discover new irradiation biomarkers.…”

Section: Discussionmentioning

confidence: 99%

“…24 This technique should be considered as a preliminary step before validation of new diagnostic markers. 25 It is a powerful method to discover new irradiation biomarkers. Using iTRAQ, we screened 17 common differentially expressed proteins in the three experimental dose groups.…”

Section: ■ Discussionmentioning

confidence: 99%

iTRAQ-Based Proteomic Profiling of Potential Biomarkers in Rat Serum for Uranium Tailing Suspension Intratracheal Instillation

Liang

Cui

et al. 2020

J. Proteome Res.

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Protection against low-dose ionizing radiation is of great significance. Uranium tailings are formed as a byproduct of uranium mining and a potential risk to organisms. In this study, we identified potential biomarkers associated with exposure to low-dose radiation from uranium tailings. We established a Wistar rat model of low dose rate irradiation by intratracheal instillation of a uranium tailing suspension. We observed pathological changes in the liver, lung, and kidney tissues of the rats. Using isobaric tags for relative and absolute quantification, we screened 17 common differentially expressed proteins in three dose groups. We chose alpha-1 antiproteinase (Serpina1), keratin 17 (Krt17), and aldehyde dehydrogenase (Aldh3a1) for further investigation. Our data showed that expression of Serpina1, Krt17, and Aldh3a1 had changed after the intratracheal instillation in rats, which may be potential biomarkers for uranium tailing low-dose irradiation. However, the underlying mechanisms require further investigation.

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“…The former are believed to be required for catalysis which functions in the transfer of electrons to the respiratory chain required for enzymatic activity to fight against aspergillosis. Noteworthy in the same study, no markers were shared with blood samples from infected humans, except one, so called Testis expressed 11 (Fragment) protein, >3-fold increased in both species and involved in the cell organization and biogenesis; metabolic process; regulation of biological process Desoubeaux et al, 2019). Further studies are warranted to validate the relevancy of these proteins in the diagnostic process of aspergillosis.…”

Section: What Are the Diagnostic Means In Dolphins?mentioning

confidence: 94%

“…To understand more about the pathophysiology of infection and possibly identify new tools, some studies have used mass spectrometry to study aspergillosis in dolphins ( Desoubeaux et al., 2019 ). Several over-represented proteins which play a role in the adaptive immune response were identified, including MHC (major histocompatibility complex) proteins and others involved in catalytic activity like the NADPH-ubiquinone oxido-reductases or cytochrome b.…”

Section: What Are the Diagnostic Means In Dolphins?mentioning

confidence: 99%

Challenges to establish the diagnosis of aspergillosis in non-laboratory animals: looking for alternatives in veterinary medicine and demonstration of feasibility through two concrete examples in penguins and dolphins

Désoubeaux

Cray

Chesnay

2022

Front. Cell. Infect. Microbiol.

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Aspergillosis remains difficult to diagnose in animals. Laboratory-based assays are far less developed than those for human medicine, and only few studies have been completed to validate their utility in routine veterinary diagnostics. To overcome the current limitations, veterinarians and researchers have to propose alternative methods including extrapolating from human diagnostic tools and using innovative technology. In the present overview, two specific examples were complementarily addressed in penguins and dolphins to illustrate how is challenging the diagnosis of aspergillosis in animals. Specific focus will be made on the novel application of simple testing in blood based on serological assays or protein electrophoresis and on the new information garnered from metabolomics/proteomics to discover potential new biomarkers. In conclusion, while the diagnostic approach of aspergillosis in veterinary medicine cannot be directly taken from options developed for human medicine, it can certainly serve as inspiration.

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Labeled quantitative mass spectrometry to study the host response during aspergillosis in the common bottlenose dolphin (Tursiops truncatus)

Cited by 5 publications

References 32 publications

The plasma phospholipidome of Tursiops truncatus: From physiological insight to the design of prospective tools for managed cetacean monitorization

The plasma phospholipidome of Tursiops truncatus: From physiological insight to the design of prospective tools for managed cetacean monitorization

iTRAQ-Based Proteomic Profiling of Potential Biomarkers in Rat Serum for Uranium Tailing Suspension Intratracheal Instillation

Challenges to establish the diagnosis of aspergillosis in non-laboratory animals: looking for alternatives in veterinary medicine and demonstration of feasibility through two concrete examples in penguins and dolphins

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