Label-Free Voltammetric Aptasensor for the Sensitive Detection of Microcystin-LR Using Graphene-Modified Electrodes

Eissa, Shimaa; Ng, Andy; Siaj, Mohamed; Zourob, Mohammed

doi:10.1021/ac501335k

Cited by 128 publications

(87 citation statements)

References 39 publications

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“…The enzyme-based biosensors are disadvantageous when compared with the aptatoxisensor, as the recognition enzyme at the center of the biosensor activity usually becomes inhibited by contaminants present in the water sample and so returns in faulty results. In addition, these biosensors are not usually efficient at discriminating between the various cyanotoxin analogs (e.g., nodularins and MC-LR) present in the same sample [44,45,46,47]. The novel aptatoxisensor presented in this study does not suffer from such limitations.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Aptatoxisensor Responses on Nanocomposites Containing Electro-Deposited Silver Nanoparticles on Poly(Propyleneimine) Dendrimer for the Detection of Microcystin-LR in Freshwater

Bilibana

Williams

Rassie

et al. 2016

Sensors

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A sensitive and reagentless electrochemical aptatoxisensor was developed on cobalt (II) salicylaldiimine metallodendrimer (SDD–Co(II)) doped with electro-synthesized silver nanoparticles (AgNPs) for microcystin-LR (L, l-leucine; R, l-arginine), or MC-LR, detection in the nanomolar range. The GCE|SDD–Co(II)|AgNPs aptatoxisensor was fabricated with 5’ thiolated aptamer through self-assembly on the modified surface of the glassy carbon electrode (GCE) and the electronic response was measured using cyclic voltammetry (CV). Specific binding of MC-LR with the aptamer on GCE|SDD–Co(II)|AgNPs aptatoxisensor caused the formation of a complex that resulted in steric hindrance and electrostatic repulsion culminating in variation of the corresponding peak current of the electrochemical probe. The aptatoxisensor showed a linear response for MC-LR between 0.1 and 1.1 µg·L−1 and the calculated limit of detection (LOD) was 0.04 µg·L−1. In the detection of MC-LR in water samples, the aptatoxisensor proved to be highly sensitive and stable, performed well in the presence of interfering analog and was comparable to the conventional analytical techniques. The results demonstrate that the constructed MC-LR aptatoxisensor is a suitable device for routine quantification of MC-LR in freshwater and environmental samples.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Aptatoxisensor Responses on Nanocomposites Containing Electro-Deposited Silver Nanoparticles on Poly(Propyleneimine) Dendrimer for the Detection of Microcystin-LR in Freshwater

Bilibana

Williams

Rassie

et al. 2016

Sensors

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“…A linear range of 0.1 pM to 1.0 nM was obtained with a LOD of 1.9 pM. The aptasensor was applied to the analysis of spiked tap water and fish extracts [49].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Affinity Biosensors in Food Safety

2017

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Safety and quality are key issues of today's food industry. Since the food chain is becoming more and more complex, powerful analytical methods are required to verify the performance of food safety and quality systems. Indeed, such methods require high sensitivity, selectivity, ability for rapid implementation and capability of automatic screening. Electroanalytical chemistry has, for decades, played a relevant role in food safety and quality assessment, taking more and more significance over time in the solution of analytical problems. At present, the implementation of electrochemical methods in the food is evident. This is in a large part due to the relevant results obtained by combining the attractive advantages of electrochemical transduction strategies (in terms of relatively simple hardware, versatility, interface with automatic logging and feasibility of application outside the laboratory environment) with those from biosensors technology. Important examples of enzyme electrochemical biosensors are those dedicated to the determination of glucose, alcohol or cholesterol are important examples. In addition, other types of different electrochemical biosensing approaches have emerged strongly in the last years. Among these, the strategies involving affinity interactions have been shown to possess a large number of applications. Therefore, electrochemical immunosensors and DNA-based biosensors have been widely used to determine major and minor components in foodstuffs, providing sufficient data to evaluate food freshness, the quality of raw materials, or the origin of samples, as well as to determine a variety of compounds at trace levels related to food safety such as micotoxins, allergens, drugs residues or pathogen microorganisms. This review discusses some critical examples of the latest advances in this area, pointing out relevant methodologies related to the measurement techniques, including the use of nanostructured electrodes and strategies for signal amplification.

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“…However, the tedious procedures for the preparation of the antibodies may limit their wide applications. Recently, alternative biosensors using aptamers as bioreceptors have been proposed [15,16]. Unfortunately, the selected aptamers are available only to a few microcystin congeners.…”

Section: Introductionmentioning

confidence: 99%

Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR

Ding

Wang

et al. 2016

Sensors and Actuators B: Chemical

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a b s t r a c tWe report here on the development of a chronopotentiometric assay for microcystin-LR based on enzymatic inhibition. The inhibition of protein phosphatase by microcystin-LR can be sensed potentiometrically by using 4-nitrophenyl phosphate as an enzyme substrate. A solid-contact ion-selective electrode (ISE) with poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) as a transduction layer has been designed for potentiometric biosensing using the pulsed galvanastatic technique. By applying an anodic current, the enzymatic generated p-nitrophenol can be extracted into the polymeric membrane with tetradodecylammonium tetrakis(4-chlorophenyl)-borate to produce the chronopotentiometric signal. Meanwhile, a controlled voltage was applied to refresh the membrane for multiple consecutive measurements. The proposed potentiometric assay showed a linear response for microcystin-LR in the range 1-100 g/L with a detection limit of 0.5 g/L (3 ). We believe that the proposed method can be employed for sensitive, rapid and reliable determination of analytes involved in enzyme inhibition.

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Label-Free Voltammetric Aptasensor for the Sensitive Detection of Microcystin-LR Using Graphene-Modified Electrodes

Cited by 128 publications

References 39 publications

Electrochemical Aptatoxisensor Responses on Nanocomposites Containing Electro-Deposited Silver Nanoparticles on Poly(Propyleneimine) Dendrimer for the Detection of Microcystin-LR in Freshwater

Electrochemical Aptatoxisensor Responses on Nanocomposites Containing Electro-Deposited Silver Nanoparticles on Poly(Propyleneimine) Dendrimer for the Detection of Microcystin-LR in Freshwater

Electrochemical Affinity Biosensors in Food Safety

Pulsed galvanostatic control of a solid-contact ion-selective electrode for potentiometric biosensing of microcystin-LR

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