Label-free tomographic imaging of nanodiamonds in living cells

Ikliptikawati, Dini Kurnia; Hazawa, Masaharu; So, Frederick T.-K.; Terada, Daiki; Kobayashi, Akito; Segawa, Takuya F.; Shirakawa, Masahiro; Wong, Richard W.

doi:10.1016/j.diamond.2021.108517

Cited by 8 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further investigations using holotomographic microscopes have observed the internalization of different nanostructures and how these are distributed in cells [66,67]. For example, the internalization of gold nanoparticles resulted in lipid droplet generation within macrophages, indicative of a morphology activation and the conversion of macrophages to phagocytes [24].…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility Evaluation of TiO2, Fe3O4, and TiO2/Fe3O4 Nanomaterials: Insights into Potential Toxic Effects in Erythrocytes and HepG2 Cells

Paramo,

Jiménez-Chávez,

Medina-Ramirez

et al. 2023

Nanomaterials

View full text Add to dashboard Cite

Nanomaterials such as titanium dioxide and magnetite are increasingly used in several fields, such as water remediation and agriculture. However, this has raised environmental concerns due to potential exposure to organisms like humans. Nanomaterials can cause adverse interactions depending on physicochemical characteristics, like size, morphology, and composition, when interacting with living beings. To ensure safe use and prevent the risk of exposure to nanomaterials, their biocompatibility must be assessed. In vitro cell cultures are beneficial for assessing nanomaterial–cell interactions due to their easy handling. The present study evaluated the biocompatibility of TiO2, Fe3O4, and TiO2/Fe3O4 nanomaterials thermally treated at 350 °C and 450 °C in erythrocytes and HepG2 cells. According to the hemolysis experiments, non-thermally treated NMs are toxic (>5% hemolysis), but their thermally treated counterparts do not present toxicity (<2%). This behavior indicates that the toxicity derives from some precursor (solvent or surfactant) used in the synthesis of the nanomaterials. All the thermally treated nanomaterials did not show hemolytic activity under different conditions, such as low-light exposure or the absence of blood plasma proteins. In contrast, non-thermally treated nanomaterials showed a high hemolytic behavior, which was reduced after the purification (washing and thermal treatment) of nanomaterials, indicating the presence of surfactant residue used during synthesis. An MTS cell viability assay shows that calcined nanomaterials do not reduce cell viability (>11%) during 24 h of exposure. On the other hand, a lactate dehydrogenase leakage assay resulted in a higher variability, indicating that several nanomaterials did not cause an increase in cell death as compared to the control. However, a holotomographic microscopy analysis reveals a high accumulation of nanomaterials in the cell structure at a low concentration (10 µg mL−1), altering cell morphology, which could lead to cell membrane damage and cell viability reduction.

show abstract

Section: Discussionmentioning

confidence: 99%

Biocompatibility Evaluation of TiO2, Fe3O4, and TiO2/Fe3O4 Nanomaterials: Insights into Potential Toxic Effects in Erythrocytes and HepG2 Cells

Paramo,

Jiménez-Chávez,

Medina-Ramirez

et al. 2023

Nanomaterials

View full text Add to dashboard Cite

show abstract

“…Four to six drops of each nanoparticle solution was added to a cuvette and diluted with Milli-Q water to approximately 85% full, then sonicated for 1-2 minutes. Refractive indices (0.20, 2.42, 2.13 for Au, ND, and ZrO 2 , respectively) and absorption constants (2.43, 0.00, 0.01 for Au, ND, and ZrO 2 , respectively) were based on literature values [60][61][62][63]. Particle size distribution curves were analyzed with the curve fitting software Fityk.…”

Section: Nanoparticle Solution Characterizationmentioning

confidence: 99%

Influence of Nanoparticle Chemical Composition on <i>In Situ</i> Hydrogel Friction

Bovia,

Gleeson,

Buckley

et al. 2023

Tribology Online

View full text Add to dashboard Cite

Nanoparticles are promising candidates as direct therapeutics and delivery systems for osteoarthritis treatments, primarily via intraarticular injection, but little is known about the impact on sliding behavior for a soft material surface like cartilage that would be encountered in a joint. Nanoparticle additives have primarily been studied in the context of hard material interfaces, such as metals or metal oxides, where different lubricating or anti-wear mechanisms depend sensitively on chemical composition, size, and concentration.To understand what nanoparticle parameters influence in situ (in a fluid environment) frictional behavior of soft materials, polyacrylamide (PAM) hydrogels were used as a model soft material platform. Friction tests were conducted in a rheometer with a tribology adapter, with PAM hydrogels molded in a petri dish and immersed in different nanoparticle containing fluid environments. A range of nanoparticle compositions were selected to compare broad categories: gold (metal) with a citrate capping ligand, nanodiamond (carbon), and zirconium dioxide (metal oxide). Comparing surface chemistry, concentration, and degree of aggregation, both nanoparticle surface chemistry and nanoparticle solution viscosity were found to modulate in situ hydrogel friction.

show abstract

“…Diamond nanoparticles are highly biocompatible 7 , 8 , which makes them useful for medical purposes and biological applications as antimicrobial agents, novel diagnostic approaches, or treatments of infectious diseases 9 . They can be utilized in gene/drug delivery 10 , nanoscale magnetometry 11 , label-free imaging 12 , and as live-cell nanothermometers in biosensing 13 . Owing to the chemical inertness and large surface area of diamond particles and the possibility of surface functionalization with medical and biological agents, nanodiamonds can play a remarkable role in drug delivery 14 and microdiamonds (MDs) are also promising for medical applications 15 , 16 .…”

Section: Introductionmentioning

confidence: 99%

Cell-particles interaction – selective uptake and transport of microdiamonds

Ebrahimi,

Gawlik,

Wojciechowski

et al. 2024

Commun Biol

View full text Add to dashboard Cite

Diamond particles have recently emerged as novel agents in cellular studies because of their superb biocompatibility. Their unique characteristics, including small size and the presence of fluorescent color centers, stimulate many important applications. However, the mechanism of interaction between cells and diamond particles—uptake, transport, and final localization within cells—is not yet fully understood. Herein, we show a novel, to the best of our knowledge, cell behavior wherein cells actively target and uptake diamond particles rather than latex beads from their surroundings, followed by their active transport within cells. Furthermore, we demonstrate that myosin-X is involved in cell-particle interaction, while myosin-II does not participate in particle uptake and transport. These results can have important implications for drug delivery and improve sensing methods that use diamond particles.

show abstract

Label-free tomographic imaging of nanodiamonds in living cells

Cited by 8 publications

References 33 publications

Biocompatibility Evaluation of TiO2, Fe3O4, and TiO2/Fe3O4 Nanomaterials: Insights into Potential Toxic Effects in Erythrocytes and HepG2 Cells

Biocompatibility Evaluation of TiO2, Fe3O4, and TiO2/Fe3O4 Nanomaterials: Insights into Potential Toxic Effects in Erythrocytes and HepG2 Cells

Influence of Nanoparticle Chemical Composition on <i>In Situ</i> Hydrogel Friction

Cell-particles interaction – selective uptake and transport of microdiamonds

Contact Info

Product

Resources

About