Label-free three-dimensional observations and quantitative characterisation of on-chip vasculogenesis using optical diffraction tomography

Lee, Chungha; Kim, Seung Gyu; Hugonnet, Hervé; Park, WeiSun; Jeon, Jessie S.; Park, YongKeun

doi:10.1039/d0lc01061h

Cited by 20 publications

(10 citation statements)

References 54 publications

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“…A complementary metal oxide semiconductor sensor array is employed to detect the fluorescence collected by the diffractive microlens array, which shows promising applications in flow cytometry and fluorescence spectroscopy. In addition, many other DOEs have been applied to MNOSs with various applications, including microspectroscopy, on-chip tomography, particle tracing, manipulations, etc . − …”

Section: Summary Of Micro/nano-optics-based Sensorsmentioning

confidence: 99%

Metasurface Micro/Nano-Optical Sensors: Principles and Applications

et al. 2022

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Metasurfaces are 2D artificial materials consisting of arrays of metamolecules, which are exquisitely designed to manipulate light in terms of amplitude, phase, and polarization state with spatial resolutions at the subwavelength scale. Traditional micro/nano-optical sensors (MNOSs) pursue high sensitivity through strongly localized optical fields based on diffractive and refractive optics, microcavities, and interferometers. Although detections of ultra-low concentrations of analytes have already been demonstrated, the label-free sensing and recognition of complex and unknown samples remain challenging, requiring multiple readouts from sensors, e.g., refractive index, absorption/emission spectrum, chirality, etc. Additionally, the reliability of detecting large, inhomogeneous biosamples may be compromised by the limited near-field sensing area from the localization of light. Here, we review recent advances in metasurface-based MNOSs and compare them with counterparts using micro-optics from aspects of physics, working principles, and applications. By virtue of underlying the physics and design flexibilities of metasurfaces, MNOSs have now been endowed with superb performances and advanced functionalities, leading toward highly integrated smart sensing platforms.

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Section: Summary Of Micro/nano-optics-based Sensorsmentioning

confidence: 99%

Metasurface Micro/Nano-Optical Sensors: Principles and Applications

et al. 2022

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“…33 The used imaging system is based on the Mach−Zehnder interferometer, equipped with a digital micromirror device (DMD). 34,35 A coherent laser beam (λ = 457 nm, Cobolt Twist, Cobolt, Sweden) was split into reference and sample beams using a beam splitter. The sample beam was used to illuminate a sample through a long working distance condenser (LUMFLN60XW, Olympus, NA = 1.1); the scattered light from a sample was then collected by an objective lens (UMFLN60XW, Olympus, NA = 1.1).…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Fluid–Matrix Interface Triggers a Heterogeneous Activation of Macrophages

Kim

Lee

et al. 2020

ACS Appl. Bio Mater.

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Spontaneous activation of macrophages in response to inflammation is a key part of innate immunity and host defense. Macrophages represent a heterogeneous population of cells with different phenotypic profiles performing distinct functions in host defense. Although a spectrum of macrophage activation stages exists in an inflamed region, the effect of local physical conditions on the heterotypic activation of macrophages is unknown. Here, we introduce an in vivo fluid–matrix interface analogous culture platform, an asymmetric microenvironment, facilitating the formation of macrophage aggregates (MAs). Macrophages were self-assembled to form MAs of ∼100 μm diameter at the collagen matrix–medium interface upon phorbol-12-myristate-13-acetate treatment. The macrophages within the half-embedded MAs into the matrix were heterogeneously activated, resulting in inhomogeneous cell–cell and cell–matrix interactions within the aggregates. Our demonstration may aid in a better understanding of the acquisition of macrophage heterogeneity in response to tissue-specific microenvironments.

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“…Optical diffraction tomography (ODT), the quantitative refractive index imaging technique, was developed to provide three-dimensional cell structures [8][9][10][11][12] . However, the disadvantage is that the projected twodimensional images can be distorted due to internal diffraction and refraction of the object, which limits the monitoring of lively moving cells in both the short-and long-term 13 .…”

Section: Introductionmentioning

confidence: 99%

“…Conventionally, the refractive index (RI) has played an important role in the enhanced visualization of transparent living cells using phase contrast microscopy or differential interference microscopy, which could enhance contrast due to optical interferometry [4][5][6][7]. Optical diffraction tomography, the quantitative RI imaging technique, was developed to provide 3D cell structures [8][9][10][11][12]. However, the disadvantage is that the projected 2D images can be distorted due to internal diffraction and refraction of the object, which limits the monitoring of lively moving cells in both the short-and long-term [13].…”

Section: Introductionmentioning

confidence: 99%

Miniaturized optimal incident light angle‐fitted dark field system for contrast‐enhanced real‐time monitoring of 2D/3D‐projected cell motions

Jang

Han

Song

et al. 2022

Journal of Biophotonics

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In the eld of biology, dark eld microscopy provides superior insight into cells and subcellular structures.However, most dark eld microscopes are equipped with a dark eld lter and a light source on a 2Dbased specimen, so only a at sample can be observed in a limited space. We propose a compact cell monitoring system with built-in dark eld lter with an optimized incident angle of the light source to provide real-time cell imaging and spatial cell monitoring. 2D/3D projected dark eld images are optimized for 2D/3D samples as they rely on dark eld lters, and incident light. 2D projection imaging was implemented using a modular condenser lens to acquire high-contrast images. This enabled the long-term monitoring of cells, and the real-time monitoring of cell division and death. This system was able to image, by 2D projection, cells on the surface thinly coated with multi-walled carbon nanotubes, as well as living cells that migrated along the surface of glass beads and hydrogel droplets with a diameter of about 160 μm. The Optimal incident light angle-tted dark eld system combines high-contrast imaging sensitivity and high spatial resolution to even image cells on three-dimensional surfaces.

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Label-free three-dimensional observations and quantitative characterisation of on-chip vasculogenesis using optical diffraction tomography

Abstract: Label-free, three-dimensional (3D) quantitative observations of on-chip vasculogenesis were achieved using optical diffraction tomography.

Cited by 20 publications

References 54 publications

Metasurface Micro/Nano-Optical Sensors: Principles and Applications

Metasurface Micro/Nano-Optical Sensors: Principles and Applications

Fluid–Matrix Interface Triggers a Heterogeneous Activation of Macrophages

Miniaturized optimal incident light angle‐fitted dark field system for contrast‐enhanced real‐time monitoring of 2D/3D‐projected cell motions

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