Label-free sensing of thrombin based on quantum dots and thrombin binding aptamer

Zhang, Xiangyuan; Hu, Ruoxin; Shao, Ning

doi:10.1016/j.talanta.2013.01.003

Cited by 19 publications

(10 citation statements)

References 45 publications

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“…Another similar example for the detection of thrombin has been reported in 2013 as a facile and sensitive label-free homogeneous assay. In this approach, Zhang et al used CdTe quantum dots (QDs), Cd 2+ ions and the TBA 15 [174]. The QDs can be "activated" with fluorescence enhancement by adding extra Cd 2+ to the solution in a basic medium; the positively charged Cd 2+ -activated CdTe QDs interact with the negatively charged TBA, leading to fluorescence quenching; when thrombin is added, TBA forms a G-quadruplex structure through specific interaction with its target, releasing the QDs with a recovery of the fluorescence intensity (Fig.…”

Section: Thrombin Detection By Tba and Its Variants: A Biologically Rmentioning

confidence: 99%

G-quadruplex-based aptamers against protein targets in therapy and diagnostics

Platella

Riccardi

Montesarchio

et al. 2017

Biochimica et Biophysica Acta (BBA) - General Subjects

137

116

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Nucleic acid aptamers are single-stranded DNA or RNA molecules identified to recognize with high affinity specific targets including proteins, small molecules, ions, whole cells and even entire organisms, such as viruses or bacteria. They can be identified from combinatorial libraries of DNA or RNA oligonucleotides by SELEX technology, an in vitro iterative selection procedure consisting of binding (capture), partitioning and amplification steps. Remarkably, many of the aptamers selected against biologically relevant protein targets are G-rich sequences that can fold into stable G-quadruplex (G4) structures. Aiming at disseminating novel inspiring ideas within the scientific community in the field of G4-structures, the emphasis of this review is placed on: 1) recent advancements in SELEX technology for the efficient and rapid identification of new candidate aptamers (introduction of microfluidic systems and next generation sequencing); 2) recurrence of G4 structures in aptamers selected by SELEX against biologically relevant protein targets; 3) discovery of several G4-forming motifs in important regulatory regions of the human or viral genome bound by endogenous proteins, which per se can result into potential aptamers; 4) an updated overview of G4-based aptamers with therapeutic potential and 5) a discussion on the most attractive G4-based aptamers for diagnostic applications. This article is part of a Special Issue entitled "G-quadruplex" Guest Editor: Dr. Concetta Giancola and Dr. Daniela Montesarchio.

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Section: Thrombin Detection By Tba and Its Variants: A Biologically Rmentioning

confidence: 99%

G-quadruplex-based aptamers against protein targets in therapy and diagnostics

Platella

Riccardi

Montesarchio

et al. 2017

Biochimica et Biophysica Acta (BBA) - General Subjects

137

116

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“…Label-free, “signal-on” sensing strategies. Many effective label-free, “signal-on” sensing strategies have been reported in the literature, as those described by Yan et al [ 95 ] and Zhang et al [ 96 ]. More recently, a novel, label-free thrombin detection assay has been developed based on the fluorescence resonance energy transfer (FRET) from a cationic conjugated polymer (CCP), acting as the energy donor, to an Ir(III) complex, acting as the acceptor ( Figure 6 ).…”

Section: Aptamer-based Fluorescent Systems For the Specific Recognmentioning

confidence: 99%

Fluorescence Sensing Using DNA Aptamers in Cancer Research and Clinical Diagnostics

Musumeci

Platella

Riccardi

et al. 2017

Cancers

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Among the various advantages of aptamers over antibodies, remarkable is their ability to tolerate a large number of chemical modifications within their backbone or at the termini without losing significant activity. Indeed, aptamers can be easily equipped with a wide variety of reporter groups or coupled to different carriers, nanoparticles, or other biomolecules, thus producing valuable molecular recognition tools effective for diagnostic and therapeutic purposes. This review reports an updated overview on fluorescent DNA aptamers, designed to recognize significant cancer biomarkers both in soluble or membrane-bound form. In many examples, the aptamer secondary structure switches induced by target recognition are suitably translated in a detectable fluorescent signal using either fluorescently-labelled or label-free aptamers. The fluorescence emission changes, producing an enhancement (“signal-on”) or a quenching (“signal-off”) effect, directly reflect the extent of the binding, thereby allowing for quantitative determination of the target in bioanalytical assays. Furthermore, several aptamers conjugated to fluorescent probes proved to be effective for applications in tumour diagnosis and intraoperative surgery, producing tumour-type specific, non-invasive in vivo imaging tools for cancer pre- and post-treatment assessment.

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“…This method showed a linear response for thrombin ranging from 1.4 nmol•L -1 to 21 nmol•L -1 with an LOD of 0.7 nmol•L -1 . [99] Recently, a novel exciton energy transferbased ultra sensitive fluorescent sensing strategy for the detection of thrombin has been established using split aptamer-programmed self-assembly of QDs ( Figure 6). The sensitivity of this assay was much better than that of the plasmonic coupling-based colorimetric methods, which resulted in an extremely low detection limit of 15 pmol•L -1 for thrombin.…”

Section: Ions Small Molecules and Protein Detectionmentioning

confidence: 99%

DNA Functionalized Fluorescent Quantum Dots for Bioanalytical Applications

Zhang

Ding

et al. 2016

Chin. J. Chem.

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As a newly developed fluorescent probe, DNA functionalized quantum dots (QDs) with unique optical properties play a critical role in the bioanalytical applications. The present review provides an overview on the conjugation technologies of DNA to QDs, including electrostatic attraction, covalent attachment, specific effect and direct synthesis. In addition, we summarize recent advances on DNA functionalized QDs in the bioapplications, such as targeting nucleic acids, ions, small molecules and the protein detection, cell imaging and disease diagnosis. Finally, we envision aspects concerning current challenges on the DNA functionalized fluorescent QDs.

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Label-free sensing of thrombin based on quantum dots and thrombin binding aptamer

Cited by 19 publications

References 45 publications

G-quadruplex-based aptamers against protein targets in therapy and diagnostics

G-quadruplex-based aptamers against protein targets in therapy and diagnostics

Fluorescence Sensing Using DNA Aptamers in Cancer Research and Clinical Diagnostics

DNA Functionalized Fluorescent Quantum Dots for Bioanalytical Applications

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