“…In one approach, samples in MWPs are reformatted into segmented droplets of nanoliter volume inside tubing by using aspiration to sequentially draw up plugs of sample and carrier fluid. [15,16,33–36] Microfluidic droplets can then undergo further manipulations such as mixing,[37–41] merging,[42–46] splitting,[43,47–50] addition,[35,37,38,51,52] incubation and extraction,[15,53–59] which can enable entire assays to be performed at small scale. Indeed, this approach has been used with fluorescence detection to screen 704 compounds against protein tyrosine phosphatase and high-resolution dose response curves were obtained.…”