Label-free reflectometric interference microchip biosensor based on nanoporous alumina for detection of circulating tumour cells

Kumeria, Tushar; Kurkuri, Mahaveer D.; Diener, Kerrilyn R.; Parkinson, Luke; Lošić, Dušan

doi:10.1016/j.bios.2012.02.038

Cited by 110 publications

(84 citation statements)

References 39 publications

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“…A wealth of applications have been proposed since then, for instance to detect gold ions with a limit of detection of 0.1 μM in water by exploiting the affinity of the metal ions for the surface functionalization agent MPTES [120]. The same technique is also applicable to more sophisticated substances and even to tumor cells as for instance in [121] where by the functionalization with biotinylated anti-EpCAM antibody that specifically binds to such cancer cells are able to detect down to 1000 cells/ml in blood serum with a sample volume as small as 50 μl. Besides to sensing the presence of biological molecules in aqueous media, the method can be used to monitor the release of drugs loaded onto p-AAO matrices in a flowing medium.…”

Section: Reflection Interference Spectroscopymentioning

confidence: 98%

Optical Properties of Nanoporous Anodic Alumina and Derived Applications

et al. 2015

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Inexpensive formation of periodically ordered structures with periodicities and feature sizes lower than 100 nm has triggered a vast amount or research in recent years. Of particular interest in nanotechnology is the nanoporous alumina, which can be produced with a self-organized arrangement of pores in the adequate anodization conditions. Most of the interest of this material is based in its outstanding physical and chemical and properties, and more specifically in its optical properties. The interaction of light with the nanostructured porous alumina gives rise to a wealth of optical properties that have their interest both in research level and also in application. In this work we aim at giving an extensive review of the published research on the optical properties of nanoporous alumina. The review will account for the different studied optical properties of this material such as the existence of a photonic stop band originated from its quasi-random nanostructure, the interferometric and light guiding properties that can be applied to biosensing, the structure nanoengineering to achieve more complex photonic behaviour such as distributed-Bragg reflectors or rugate filters, and the photoluminescence properties. In a second part, a summary of the different applications proposed on the basis of these properties, such as in biotechnology or energy will be given. IntroductionAmong the different features and properties of porous anodic alumina (p-AAO), its optical properties are of special interest and have been the subject of very intensive research. The interaction of light with p-AAO can be analyzed from different points of view: the material interaction, the porous structure interaction or the relation of geometrical features of the nanostructure (periodicity or quasi-periodicity, distance

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Section: Reflection Interference Spectroscopymentioning

confidence: 98%

Optical Properties of Nanoporous Anodic Alumina and Derived Applications

et al. 2015

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“…Such phenomena have also been observed when densely packed SAMs (MUA-gold-AAO) were attached with another type of bio-receptors. For example, Kumeria et al [7] investigated the mechanism of antibody-antigen interactions at SAMs (MUA-gold-AAO) electrode using reflectometric interference spectroscopy (RIfS). The mechanism of the system is shown in Fig.…”

Section: Self Assembly Monolayersmentioning

confidence: 99%

“…The system begins with a the formation of self-assembled monolayers (SAMs) of carboxyl-containing thiol followed by b covalent attachment of streptavidin on activated SAMs after activation with coupling (NHS/EDC) agents and finally c immobilization of biotinylated Anti-EpCAM antibodies; d scheme of binding of CTC cell on anti-EpCAM antibodies. Adapted with permission from [7] immersion of freshly prepared membrane into dilute alkylthiol in an ethanoic solution for 12-24 h at room temperature. A series of experiments were carried out by Le et al [11] for separation studies using five different kinds of thiol-modified gold coated AAO membranes.…”

Section: Thiol Surface Chemistrymentioning

confidence: 99%

Soft and Hard Surface Manipulation of Nanoporous Anodic Aluminum Oxide (AAO)

et al. 2015

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Nanoporous materials with straight channels have attracted considerable interest due to their unique physical properties and many potential applications such as separation, sensing, biomedical and electronics. For the last few decades, nanoporous alumina or anodic aluminium oxide (AAO) membrane is gaining attention due to its broad applicability in various applications. The unique properties of AAO membrane coupled with tunable surface modification and properties is playing an increasingly important platform in a diverse range of applications such as separation, energy storage, drug delivery and template synthesis, as well as biosensing, tissue engineering and catalytic studies. This chapter aims to introduce the recent advances and challenges for surface manipulation of AAO following the 'soft' and 'hard' modification strategies. The functions of these modified nanostructures materials and latest important applications are evaluated with respect to improved performance and possible implications of those strategies for the future trends of surface engineering are discussed.

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“…[102] L. F. Marsal et al and [103], where the RIfS method is applied to sense sulphur volatile gases by coating the top of the inner pore walls with a thin film of Au, which shows affinity to the gas molecules. The same structure and strategy has been also applied to the detection of circulating tumour cells by adding a self-assembled monolayer of the adequate antibodies by means of biotin-streptavidin attachment [104]. The functionalization of the PAA inner pore walls with 3-mercaptopropyl-triethoxysilane (MPTES) permits ultra sensitive detection of Au3+ ions [87].…”

Section: Biosensingmentioning

confidence: 99%

Mesoporous alumina as a biomaterial for biomedical applications

Xifré-Pérez

Ferre-Borull

Pallarès

et al. 2015

Open Material Sciences

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Porous anodic alumina (PAA) is a biomaterial based on a cost-effective electrochemical anodization of pure aluminum with unique geometrical properties, i.e., self-ordering hexagonal pore distribution, tunable pore diameters and interpore distances, and uniformity of the pores in the vertical direction (nanochannels). These remarkable properties have found important applications in several fields such as energy storage, optics, photonics, magnetism, catalysis and, in particular, in the biomedicine field. In this work, we review the current state of research and key issues on cell culture and implants, drug delivery systems with complex release profiles and specific action, and high efficiency and sensitivity biosensors with different biosensing mechanisms, all of them based on PAA. The biocompatibility, morphology of the surface, nanoestructural engineering in-depth, surface functionalization and coatings are discussed and analyzed in detail.

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Label-free reflectometric interference microchip biosensor based on nanoporous alumina for detection of circulating tumour cells

Cited by 110 publications

References 39 publications

Optical Properties of Nanoporous Anodic Alumina and Derived Applications

Optical Properties of Nanoporous Anodic Alumina and Derived Applications

Soft and Hard Surface Manipulation of Nanoporous Anodic Aluminum Oxide (AAO)

Mesoporous alumina as a biomaterial for biomedical applications

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