Label-Free Raman Spectroscopy Detects Stromal Adaptations in Premetastatic Lungs Primed by Breast Cancer

Paidi, Santosh Kumar; Rizwan, Asif; Zheng, Chao; Cheng, Maojie; Glunde, Kristine; Barman, Ishan

doi:10.1158/0008-5472.can-16-1862

Cited by 54 publications

(57 citation statements)

References 50 publications

(62 reference statements)

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“…We also conducted a negative control test for eliminating the possibility of spurious correlations driven discrimination . The spectra were assigned with different random class labels without taking into account their true class origins.…”

Section: Resultsmentioning

confidence: 99%

Raman spectroscopy for accurately characterizing biomolecular changes in androgen‐independent prostate cancer cells

Corsetti

Rabl

McGloin

et al. 2017

Journal of Biophotonics

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Metastatic prostate cancer resistant to hormonal manipulation is considered the advanced stage of the disease and leads to most cancer-related mortality. With new research focusing on modulating cancer growth, it is essential to understand the biochemical changes in cells that can then be exploited for drug discovery and for improving responsiveness to treatment. Raman spectroscopy has a high chemical specificity and can be used to detect and quantify molecular changes at the cellular level. Collection of large data sets generated from biological samples can be employed to form discriminatory algorithms for detection of subtle and early changes in cancer cells. The present study describes Raman finger printing of normal and metastatic hormone-resistant prostate cancer cells including analyses with principal component analysis and linear discrimination. Amino acid-specific signals were identified, especially loss of arginine band. Androgen-resistant prostate cancer cells presented a higher content of phenylalanine, tyrosine, DNA and Amide III in comparison to PNT2 cells, which possessed greater amounts of L-arginine and had a B conformation of DNA. The analysis utilized in this study could reliably differentiate the 2 cell lines (sensitivity 95%; specificity 88%).

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Section: Resultsmentioning

confidence: 99%

Raman spectroscopy for accurately characterizing biomolecular changes in androgen‐independent prostate cancer cells

Corsetti

Rabl

McGloin

et al. 2017

Journal of Biophotonics

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“…The quantification of changes of collagen within the primary tumor and metastatic niches has been the subject of numerous studies and it is recognized to play an important role in both cancer development and progression [19][20][21] . However, much less is known about remodelling of collagen fibers in healthy looking colon mucosa distant from the cancer.…”

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confidence: 99%

Altered organization of collagen fibers in the uninvolved human colon mucosa 10 cm and 20 cm away from the malignant tumor

Despotović

Milićević

Krmpot

et al. 2020

Sci Rep

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Remodelling of collagen fibers has been described during every phase of cancer genesis and progression. Changes in morphology and organization of collagen fibers contribute to the formation of microenvironment that favors cancer progression and development of metastasis. However, there are only few data about remodelling of collagen fibers in healthy looking mucosa distant from the cancer. Using SHG imaging, electron microscopy and specialized softwares (CT-FIRE, CurveAlign and FiberFit), we objectively visualized and quantified changes in morphology and organization of collagen fibers and investigated possible causes of collagen remodelling (change in syntheses, degradation and collagen cross-linking) in the colon mucosa 10 cm and 20 cm away from the cancer in comparison with healthy mucosa. We showed that in the lamina propria this far from the colon cancer, there were changes in collagen architecture (width, straightness, alignment of collagen fibers and collagen molecules inside fibers), increased representation of myofibroblasts and increase expression of collagen-remodelling enzymes (LOX and MMP2). Thus, the changes in organization of collagen fibers, which were already described in the cancer microenvironment, also exist in the mucosa far from the cancer, but smaller in magnitude. Extracellular matrix (ECM) is no longer considered as an inert substrate, a three-dimensional network which only "fills the spaces" between cells and provide mechanical support 1,2. Today, ECM is known to be a complex and dynamic structure, whose chemical and biophysical properties affect cell adhesion 3 , proliferation 4 morphology 5 , migration 6 , regulate tissue morphogenesis 7,8 and fluid volume in tissues 9. The most abundant component of ECM in the lamina propria of the colon mucosa is type I collagen. Remodelling of collagen fibers has been described in almost every solid cancer, including colorectal cancer. During tumor formation and progression, collagen remodelling is constantly carried out: degradation, synthesis, cross-linking of fibers, change of fiber orientation, and interaction of cells of the innate and acquired immune system with collagen fibers 10,11. Changes in morphology, representation, and organization of collagen fibers contribute to the formation of the microenvironment that favors tumor progression, primarily through its effect on cell migration and polarization 12. Also, remodelling of collagen fibers on premetastatic sites is of great importance in determination of survival and growth of disseminated cancer cells, and thus, formation of metastasis 13,14. Remodelling of collagen fibers may be a result of changes in synthesis, degradation or cross-linking. Main cells responsible for synthesis of collagen in colon mucosa are fibroblasts and myofibroblasts. The most important enzymes for degradation of collagen fibers are matrix metalloproteinases (MMPs). It has been shown that expression of MMP2 and MMP9 is increased in colorectal cancer and influences its progression and

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“…Illnesses can present themselves in many different forms. Changes in the morphology, chemical composition and biophysical parameters, such as matter density and cell dry mass, provide different windows through which the illnesses can be understood and diagnosed [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…The chemical composition of the sample can be probed by Raman spectroscopy in a noninvasive way and without the need of extrinsic labels. In the biomedical field, Raman spectroscopy has already been employed to diagnose cancer [1,2], monitor blood glucose [3], differentiate cell lines [4] and sub-compartments [6], and many other applications.…”

Section: Introductionmentioning

confidence: 99%

Raman and quantitative-phase microscope with counter-propagating beams demonstrated on HeLa cells

Tormo¹,

Khalenkow²,

Skirtach³

et al. 2019

OSA Continuum

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Raman spectroscopy probes the chemical composition of biological samples with sub-micron resolution, rendering it a powerful tool in the diagnosis of several diseases and the study unstained biological samples. However, the weak Raman signal leads to long acquisition times, unsuited to study dynamical processes or large samples. Quantitative phase microscopy can speed up the diagnosis, provide complementary data, and potentially link the Raman fingerprint of the sample with corresponding refractive indices. Here we demonstrate a 4π microscope that records both the Raman and quantitative phase information from the same sample spot.

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Label-Free Raman Spectroscopy Detects Stromal Adaptations in Premetastatic Lungs Primed by Breast Cancer

Cited by 54 publications

References 50 publications

Raman spectroscopy for accurately characterizing biomolecular changes in androgen‐independent prostate cancer cells

Raman spectroscopy for accurately characterizing biomolecular changes in androgen‐independent prostate cancer cells

Altered organization of collagen fibers in the uninvolved human colon mucosa 10 cm and 20 cm away from the malignant tumor

Raman and quantitative-phase microscope with counter-propagating beams demonstrated on HeLa cells

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