Label-free quantitative proteomics to investigate the response of strawberry fruit after controlled ozone treatment

Abstract

To elucidate the postharvest senescence of strawberry fruit in response to ozone treatment at different concentrations, a label-free quantitative proteomic approach was performed.

Effects of ozone treatment on the antioxidant capacity of postharvest strawberry

“…The strawberries were placed in an ozone precision-controlled fumigation device (2 m  1.5 m  0.8 m, with a storage capacity of 1200 L) and treated for 10 h under different concentrations of ozone (0, 10.72 mg m À3 ) every 7 d at 4 AE 1 C with 75-80% relative humidity (RH), which was independently developed by the National Agricultural Products Preservation Engineering Technology Research Center. 46 The storage period of the strawberries was 28 d, and samples were collected for physical and chemical detection aer 0, 7, 14, 21, and 28 d, for protein analysis aer 0, 7, and 21 d.…”

“…Detailed procedures for the extraction and identication of strawberry proteins, analysis, and validation of the data, and bioinformatics analysis are described in previously published articles. 46 Strawberry peptides were analyzed on a Q-Exactive HF mass spectrometer (Thermo Scientic, Waltham, MA, USA) and Ultimate 3000 system (Thermo Scientic). Tandem mass spectra were searched against Mascot 2.0 (Matrix Science, London, UK) using a UniProt sequence database.…”

“…The specic primers were designed using Primer 3.0 (ESI Table 1 †), and the method of detection was described in our previous study. 46 Total RNA was extracted from three independent samples using the MiniBEST Plant RNA Extraction Kit (TAKARA, Japan). Relative quantication of the candidate genes by qRT-PCR was carried out on an ABI StepO-nePlus Real-Time PCR System and analyzed using the comparative C t method with 18S rRNA (GenBank no.…”

Effects of ozone treatment on SOD activity and genes in postharvest cantaloupe

“…Aer the cantaloupe was pre-cooled, the OT group was transported to an ozone refrigeration fumigation device (2 m  1.5 m  0.8 m, storage capacity 1200 L) for 30 d at 4 AE 1 C with 75-80% relative humidity (RH), which was developed by the National Agricultural Products Preservation Engineering Research Center. 16 The CK group was stored at the same fumigation device for 20 d (decayed before the 30th day). Each group was treated with different concentrations of ozone (0, 15 mg m À3 ) for 2 h at 0, 10, and 20 d. The specic method of processing as described in our previous article.…”

“…Each group was treated with different concentrations of ozone (0, 15 mg m À3 ) for 2 h at 0, 10, and 20 d. The specic method of processing as described in our previous article. 16,17 On the day of sampling, the whole cantaloupe was separated into two parts, the pericarp and the pulp, and the pericarp sample was obtained 1 cm from the outermost layer to the inner, the rest was pulp sample except seeds. Both the pericarp and pulp samples were cut into small pieces, frozen in liquid nitrogen, and stored at À80 C. 6 cantaloupes were prepared at each time each group and every experiment had three times replicates.…”