Label-free quantitative proteomics in serum reveals candidate biomarkers associated with low bone mineral density in Mexican postmenopausal women

Aparicio-Bautista, Diana I.; Becerra-Cervera, Adriana; Rivera-Paredez, Berenice; Aguilar-Ordoñez, Israel; Ríos-Castro, Emmanuel; Reyes-Grajeda, Juan P.; Salmerón, Jorge; Hidalgo-Bravo, Alberto; Velázquez-Cruz, Rafael

doi:10.1007/s11357-023-00977-1

Cited by 2 publications

(1 citation statement)

References 68 publications

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“…GSN's link to osteoporosis function has been established, and it has been shown that GSN expression is increased in the serum of patients with osteoporosis. 18 IHC results indicated that GSN protein expression was significantly elevated in the OVX group compared to the Sham group. In contrast, GSN protein expression decreased and returned to normal after drug intervention.…”

Section: Discussionmentioning

confidence: 92%

Transcriptomics-based Exploration of the Mechanism of Kaempferol in the Treatment of Osteoporosis in Ovariectomized Rats

Pu,

Zhang,

Yan

et al. 2024

Natural Product Communications

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Objective: We explore the pharmacodynamic targets of kaempferol in treating osteoporosis using transcriptomics and animal experiment. Methods: Firstly, we constructed an ovariectomized rat model (OVX). The anti-osteoporosis effect of kaempferol was evaluated by bone mineral density (BMD) and hematoxylin-eosin staining comprehensively. Moreover, differential genes between groups were screened by RNA sequencing technology (RNA-seq) for transcriptomics and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways enrichment analysis were performed. Finally, partial results of transcriptomics were verified to detect the expression of the relevant gene expression by immunohistochemistry. Results: The pharmacodynamic findings indicated that the administration of kaempferol resulted in an elevation in BMD ( P < .01) and a notable enhancement in tibia microstructural indices in the experimental rats ( P < .01). The transcriptomic analysis revealed that NTN1, LTBP4, GSN, and EBF1 were identified as the principal targets for therapeutic intervention in osteoporosis. The results of animal experiments showed that kaempferol promoted osteogenesis and inhibited bone resorption by downregulating the protein expression of NTN1, LTBP4, GSN, and EBF1 ( P < .01). Conclusion: Kaempferol enhances BMD levels, retards tibial bone loss with structural deterioration in OVX model rats, and promotes bone formation while curbing bone resorption through NTN1, LTBP4, GSN, and EBF1 protein down-regulation.

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Section: Discussionmentioning

confidence: 92%

Transcriptomics-based Exploration of the Mechanism of Kaempferol in the Treatment of Osteoporosis in Ovariectomized Rats

Pu,

Zhang,

Yan

et al. 2024

Natural Product Communications

View full text Add to dashboard Cite

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Proteomics in orthopedic research: Recent studies and their translational implications

Li,

Stampas,

Komatsu

et al. 2024

Journal Orthopaedic Research

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Proteomics is a growing field that offers insights into various aspects of disease processes and therapy responses. Within the field of orthopedics, there are a variety of diseases that have a poor prognosis due to a lack of targeted curative therapy or disease modifying therapy. Other diseases have been difficult to manage in part due to lack of clinical biomarkers that offer meaningful insight into disease progression or severity. As an emerging technology, proteomics has been increasingly applied in studying bone biology and an assortment of orthopedics related diseases, such as osteoarthritis, osteosarcoma and bone tumors, osteoporosis, traumatic bone injury, spinal cord injury, hip and knee arthroplasty, and fragile healing. These efforts range from mechanistic studies for elucidating novel insights in tissue activity and metabolism to identification of candidate biomarkers for diagnosis, prognosis, and targeted treatment. The knowledge gained from these proteomic and functional studies has provided unique perspectives in studying orthopedic diseases. In this review, we seek to report on the current state of the proteomic study in the field of orthopedics, overview the advances in clinically applicable discoveries, and discuss the opportunities that may guide us for future research.

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Label-free quantitative proteomics in serum reveals candidate biomarkers associated with low bone mineral density in Mexican postmenopausal women

Cited by 2 publications

References 68 publications

Transcriptomics-based Exploration of the Mechanism of Kaempferol in the Treatment of Osteoporosis in Ovariectomized Rats

Transcriptomics-based Exploration of the Mechanism of Kaempferol in the Treatment of Osteoporosis in Ovariectomized Rats

Proteomics in orthopedic research: Recent studies and their translational implications

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