Label-free quantitative lymphocyte activation profiling using microfluidic impedance cytometry

Petchakup, Chayakorn; Hutchinson, Paul; Tay, Hui Min; Leong, Sheng Yuan; Li, King Ho Holden; Hou, Han Wei

doi:10.1016/j.snb.2021.129864

Cited by 31 publications

(22 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This suggests that HBsAg with the G145R mutation can inhibit signaling pathways associated with CD69 expression, which promotes immunosuppression and is utilized by the HBV G145R escape mutant for persistence and spread. We used very mild conditions for PHA stimulation, which are acceptable under modern protocols [56,57]. The conditions we used to stimulate the cells allowed for developing a response both in the direction of amplification and suppression.…”

Section: Discussionmentioning

confidence: 99%

Recombinant HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro

et al. 2022

View full text Add to dashboard Cite

Immune-escape hepatitis B virus (HBV) mutants play an important role in HBV spread. Recently, the multivalent vaccine Bubo®-Unigep has been developed to protect against both wild-type HBV and the most significant G145R mutant. Here, we compared the effects of recombinant HBsAg antigens, wild-type and mutated at G145R, both included in the new vaccine, on activation of a human high-density culture of peripheral blood mononuclear cells (PBMC) in vitro. The antigens were used either alone or in combination with phytohemagglutinin (PHA). None of the antigens alone affected the expression of CD40, HLA-DR or CD279. Wild-type HBsAg enhanced CD86 and CD69 expression, and induced TNF-α, IL-10, and IFN-γ, regardless of the anti-HBsAg status of donor. In the presence of PHA, wild-type HBsAg had no effect on either of the tested surface markers, but increased IFN-γ and IL-10 and inhibited IL-2. In contrast, the G145R mutant alone did not affect CD86 expression, it induced less CD69, and stimulated IL-2 along with lowering levels of TNF-α, IL-10, and IFN-γ. The G145R mutant also suppressed PHA-induced activation of CD69. The dramatic differences in the immune responses elicited by wild-type HBsAg and the G145R mutant HBsAg suggest distinct adaptive capabilities of the G145R mutant HBV.

show abstract

Section: Discussionmentioning

confidence: 99%

Recombinant HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Profiling opacity versus biophysical size that presents a characterization of peripheral blood mononuclear cells (PBMCs) treated with phytohemagglutinin (PHA). Reproduced with permission from [ 57 ]. Copyright Sensors and Actuators B: Chemical 2021.…”

Section: Single-cell Analysis Of Microfluidic Impedance Biosensorsmentioning

confidence: 99%

“…A novel impedance-based microfluidic technique was presented to analyze biophysical responses of antigen-specific T lymphocytes without labeling ( Figure 4 c) [ 57 ]. A spiral inertial microfluidic cell classifier was developed, which eliminated small inactive lymphocytes and collected activated lymphocytes at substantial success rates using the principle of hydrodynamic-based single-stream particle focusing.…”

Section: Single-cell Analysis Of Microfluidic Impedance Biosensorsmentioning

confidence: 99%

A Review of Advanced Impedance Biosensors with Microfluidic Chips for Single-Cell Analysis

et al. 2021

View full text Add to dashboard Cite

Electrical impedance biosensors combined with microfluidic devices can be used to analyze fundamental biological processes for high-throughput analysis at the single-cell scale. These specialized analytical tools can determine the effectiveness and toxicity of drugs with high sensitivity and demonstrate biological functions on a single-cell scale. Because the various parameters of the cells can be measured depending on methods of single-cell trapping, technological development ultimately determine the efficiency and performance of the sensors. Identifying the latest trends in single-cell trapping technologies afford opportunities such as new structural design and combination with other technologies. This will lead to more advanced applications towards improving measurement sensitivity to the desired target. In this review, we examined the basic principles of impedance sensors and their applications in various biological fields. In the next step, we introduced the latest trend of microfluidic chip technology for trapping single cells and summarized the important findings on the characteristics of single cells in impedance biosensor systems that successfully trapped single cells. This is expected to be used as a leading technology in cell biology, pathology, and pharmacological fields, promoting the further understanding of complex functions and mechanisms within individual cells with numerous data sampling and accurate analysis capabilities.

show abstract

“…Previous studies mainly employed multistage inertial microfluidics for improving the detection or separation accuracy. 39–42 For example, a serpentine inertial focusing unit was cascaded after a spiral inertial microfluidic sorter to align the pre-sorted cells into a train for downstream accurate impedance detection 39,40 or magnetic separation. 41 In all, a high-throughput, high-CF on-chip cell concentrator capable of concentrating rare MTCs in large-volume pleural effusions is urgently needed.…”

Section: Introductionmentioning

confidence: 99%

High-throughput concentration of rare malignant tumor cells from large-volume effusions by multistage inertial microfluidics

Xiang

Zhang

2022

Lab Chip

View full text Add to dashboard Cite

show abstract

Label-free quantitative lymphocyte activation profiling using microfluidic impedance cytometry

Cited by 31 publications

References 41 publications

Recombinant HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro

Recombinant HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro

A Review of Advanced Impedance Biosensors with Microfluidic Chips for Single-Cell Analysis

High-throughput concentration of rare malignant tumor cells from large-volume effusions by multistage inertial microfluidics

Contact Info

Product

Resources

About