Label-free optical biosensors for food and biological sensor applications

Khansili, Nishtha; Rattu, Gurdeep; Krishna, P. Murali

doi:10.1016/j.snb.2018.03.004

Cited by 243 publications

(118 citation statements)

References 106 publications

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“…The DNA biosensor response was regenerable over three successive regeneration and rehybridization cycles.DNA immobilization sites have the potential to absorb this oxidation marker and distort the reading. This limitation can be overcome by using a label-free DNA biosensor [6].The electrochemical label-free detection technique has shown excellent potential for DNA biosensors. This technique relies on conversion information from DNA base pair recognition into an electrical signal.…”

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confidence: 99%

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A Highly Sensitive Impedimetric DNA Biosensor Based on Hollow Silica Microspheres for Label-Free Determination of E. coli

Ariffin

Heng

Tan

et al. 2020

Sensors

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A novel label-free electrochemical DNA biosensor was constructed for the determination of Escherichia coli bacteria in environmental water samples. The aminated DNA probe was immobilized onto hollow silica microspheres (HSMs) functionalized with 3-aminopropyltriethoxysilane and deposited onto a screen-printed electrode (SPE) carbon paste with supported gold nanoparticles (AuNPs). The biosensor was optimized for higher specificity and sensitivity. The label-free E. coli DNA biosensor exhibited a dynamic linear response range of 1 × 10−10 µM to 1 × 10−5 µM (R2 = 0.982), with a limit of detection at 1.95 × 10−15 µM, without a redox mediator. The sensitivity of the developed DNA biosensor was comparable to the non-complementary and single-base mismatched DNA. The DNA biosensor demonstrated a stable response up to 21 days of storage at 4 ℃ and pH 7. The DNA biosensor response was regenerable over three successive regeneration and rehybridization cycles.

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mentioning

confidence: 99%

“…DNA immobilization sites have the potential to absorb this oxidation marker and distort the reading. This limitation can be overcome by using a label-free DNA biosensor [6].…”

mentioning

confidence: 99%

A Highly Sensitive Impedimetric DNA Biosensor Based on Hollow Silica Microspheres for Label-Free Determination of E. coli

Ariffin

Heng

Tan

et al. 2020

Sensors

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“…The noninvasive and high sensitivity nature of label-free biosensors helps to precisely measure the concentra-tion of am ultianalyte in ar egenerative way by preserving the intrinsic properties of the molecule. [7,8] These biosensors are classified as 1) electrochemical biosensors, 2) opticalb iosensors, and 3) acoustic biosensors. [9] There are subclassifications of these three primary biosensing techniques based on their transduction mechanisms.…”

Section: Biosensor Typesmentioning

confidence: 99%

“…Moreover, these have superior advantages over label‐based biosensors in improved selectivity, affinity, stoichiometry, kinetics, and thermodynamics. The noninvasive and high sensitivity nature of label‐free biosensors helps to precisely measure the concentration of a multianalyte in a regenerative way by preserving the intrinsic properties of the molecule . These biosensors are classified as 1) electrochemical biosensors, 2) optical biosensors, and 3) acoustic biosensors .…”

Section: Introductionmentioning

confidence: 99%

Strategies for the Development of Metallic‐Nanoparticle‐Based Label‐Free Biosensors and Their Biomedical Applications

et al. 2019

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Label‐free biosensors offer accurate sensing capabilities due to the reliable quantification of biological and biochemical processes. These devices function by establishing a dynamic interaction of analyte and receptor molecules and convert this interaction into a measurable signal through a transducer. In recent decades, label‐free biosensors have attracted attention in biomedical applications due to the ease of linking nanomaterials with bioreceptor molecules. In this review, recent advances in sensitivity, specificity, and sensing mechanism related to label‐free biosensors of metallic nanoparticles of gold, silver, aluminium, copper, and zinc oxide are presented. Selected sensing methods based on fluorescence, surface plasmon resonance, surface‐enhanced Raman scattering, metal‐enhanced fluorescence, and electrochemical sensors are discussed. New measurement techniques and rapid progress of label‐free biosensors are going to play a vital role in the real‐time detection of biomarkers in clinical samples, such as blood plasma, serum, and urine, as well as in targeted drug delivery. Future trends of these label‐free biosensing mechanisms and their development are also discussed.

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“…Especial interesting is the utilization of the plasmon-based sensing technique in a label-free manner [1,11]. In this field, the optical fiber sensors based on the surface plasmon resonance, excited in the thin metal film deposited on the top of the optical fiber, provide a range of advantages [12,13].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Surface Plasmon Fiber Sensor Sensitivity Through the Grafting of Gold Nanoparticles

et al. 2019

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The optical fibers, coated with plasmonic active metal films, represent the simple and unpretentious sensors, potentially useful for measurements of physical or chemical quantities and wide range of analytical application. All fiber-based plasmonic sensors operate on the same physical principle based on changes in the position of the plasmon absorption peak induced by a variation of surrounding medium refractive index. However, the observed spectral differences are often weak, and thus an enhancement of sensor sensitivity is strongly required. In this paper, we propose the immobilization of gold nanoparticles with sharp edges on the thin gold layer, deposited on the multimode fiber surface for improvement of the sensor functionality. The morphological and compositional changes in the gold covered fiber surface were determined by using the atomic force microscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy methods. As a result of gold nanoparticles immobilization, the pronounced plasmon energy concentration near the fiber surface occurred, thus enhancing the response of the proposed hybrid plasmonic system to the variation of ambient refractive index. The position of plasmon absorption in the case of the created plasmonic structure was shown to be more sensitive to the changes in the surrounding medium in comparison with the standard sensors based on the bare gold layer.

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Label-free optical biosensors for food and biological sensor applications

Cited by 243 publications

References 106 publications

A Highly Sensitive Impedimetric DNA Biosensor Based on Hollow Silica Microspheres for Label-Free Determination of E. coli

A Highly Sensitive Impedimetric DNA Biosensor Based on Hollow Silica Microspheres for Label-Free Determination of E. coli

Strategies for the Development of Metallic‐Nanoparticle‐Based Label‐Free Biosensors and Their Biomedical Applications

Enhancement of Surface Plasmon Fiber Sensor Sensitivity Through the Grafting of Gold Nanoparticles

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