Label-free monitoring of antibody–antigen interactions using optical microchip biosensors

Bhatta, D.; Stadden, E.; Hashem, Elham Y.; Sparrow, I.J.G.; Emmerson, G.D.

doi:10.1016/j.jim.2010.09.015

Cited by 12 publications

(6 citation statements)

References 14 publications

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“…To investigate the potency of BC‐MAP as an immobilizing agent, we firstly spotted BC‐MAP onto diverse surfaces and assessed the results using color‐image analysis. We used three transparent surfaces (glass, polypropylene, and polystyrene; Figure a) and two opaque surfaces (titanium and aluminum; Figure S2 in Supporting Information) because glass, polymers, and metals have been utilized as solid supports for antibody arrays and immunosensors 35–38. Individual MAP and BC proteins were used as controls.…”

Section: Resultsmentioning

confidence: 99%

A Mussel Adhesive Protein Fused with the BC Domain of Protein A is a Functional Linker Material that Efficiently Immobilizes Antibodies onto Diverse Surfaces

Kim

Choi

et al. 2011

Adv Funct Materials

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The efficient immobilization of antibodies onto solid surfaces is vital for the sensitivity and specificity of various immunoassays and immunosensors. A novel linker protein, BC‐MAP, is designed and produced in Escherichia coli by genetically fusing mussel adhesive protein (MAP) with two domains (B and C) of protein A (antibody‐binding protein) for efficient antibody immobilization on diverse surfaces. Through direct surface‐coating analyses, it is found that BC‐MAP successfully coats diverse surfaces including glass, polymers, and metals, but the BC domain alone does not. Importantly, antibodies are efficiently immobilized on BC‐MAP‐coated surfaces, and the immobilized antibodies interact selectively with their corresponding antigen. Quartz‐crystal‐microbalance analyses show that BC‐MAP has excellent antibody‐binding ability compared to that of BC protein on gold surfaces. These results demonstrate that the MAP domain, with uniquely strong underwater adhesive properties, plays a role in the direct and efficient coating of BC‐MAP molecules onto diverse surfaces that lack additional surface treatment, and the BC domain of BC‐MAP contributes to the selective and oriented immobilization of antibodies on BC‐MAP‐coated surfaces. Thus, the BC‐MAP fusion protein could be a valuable novel linker material for the facile and efficient immobilization of antibodies onto diverse solid supports.

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Section: Resultsmentioning

confidence: 99%

A Mussel Adhesive Protein Fused with the BC Domain of Protein A is a Functional Linker Material that Efficiently Immobilizes Antibodies onto Diverse Surfaces

Kim

Choi

et al. 2011

Adv Funct Materials

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“…Sensing platforms using SPR can measure modest changes in the refractive index emanating from the interactions between two bioentities close to the surface of the plasmonic "element". The binding kinetics near the surface can be measured in real time [59][60][61]. While aggregation-based immunoassays use simple instrumentation, biosensor sensitivity is limited by the number of aggregation events and require a long incubation period to ensure maximized signals.…”

Section: Detection Of Thrombin For Blood Clottingmentioning

confidence: 99%

Plasmonic Sensors for Disease Detection - A Review

Barizuddin¹,

S²

2016

J Nanomed Nanotechnol

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Surface Plasmon Resonance (SPR) and Localized Surface Plasmon Resonance (LSPR) have become powerful bio-sensing techniques. These techniques allow for rapid and ultra-sensitive detection of biological analytes with applications in medical diagnostics, environmental monitoring, and food safety. Plasmonic sensors are fabricated using metal nanoparticles, metal nanostructures or a combination of both. With major advancements in the area of nano synthesis, the development of plasmonic sensors has expanded significantly. Plasmonic platforms offer a viable substitute to detection techniques such as Enzyme Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) in some cases and complement them in many others. Most of the state-of-the-art plasmonic biosensors can measure binding affinity and kinetic rates in real time from any molecule that can change its refractive index. Other developments in plasmonics include the use of metamaterials that operate in the visible and infra-red region. In this review, we will briefly cover the developments in the last few years in plasmonics as it relates to disease detection.

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“…Biosensors promise to improve pathogen detection, providing a wide field of application and permitting sensitive, specific, and rapid analysis of diverse specimens . Specifically, label‐free optical biosensors such as planar Bragg grating sensors (PBGs), among others, emerged as alternatives to traditional clinical analysis methods, allowing to reduce costs, improve handling and avoid labeling .…”

Section: Introductionmentioning

confidence: 99%

“…Several authors have evaluated antibody modified sensors for the detection of biological entities including bacteria . However, in most studies antibodies were covalently attached to the sensor surface, permitting only single use or requiring laborious and/or harsh cleaning procedures prior to subsequent analysis.…”

Section: Introductionmentioning

confidence: 99%

“…Towards this end, antibody conjugation through interaction of the Fc part of antibodies with protein A or G would represent a straightforward approach, allowing regeneration of the sensor through cleavage of protein A/G‐antibody interaction, e.g. by incubation in acidic guanidine solution . But, both costs and high molecular weight of the linker molecules represent significant drawbacks of this strategy.…”

Section: Introductionmentioning

confidence: 99%

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Surface functionalization allowing repetitive use of optical sensors for real‐time detection of antibody‐bacteria interaction

Kutscher

Rosenberger

Schmauß

et al. 2015

Journal of Biophotonics

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In this study, sensor surface functionalization allowing the repetitive use of a sensing device was evaluated for antibody-based detection of living bacteria using an optical planar Bragg grating sensor. To achieve regenerable immobilization of bacteria specific antibodies, the heterobifunctional cross-linker N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) was linked to an aminosilanized sensor surface and subsequently reduced to expose sulfhydryl groups enabling the covalent conjugation of SPDP-activated antibodies via disulfide bonds. The immobilization of a capture antibody specific for Staphylococcus aureus on the sensor surface as well as specific binding of S. aureus could be monitored, highlighting the applicability of optical sensors for the specific detection of large biological structures. Reusability of bacteria saturated sensors was successfully demonstrated by cleaving the antibody along with bound bacteria through reduction of disulfide bonds and subsequent re-functionalization with activated antibody, resulting in comparable sensitivity towards S. aureus.

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Label-free monitoring of antibody–antigen interactions using optical microchip biosensors

Cited by 12 publications

References 14 publications

A Mussel Adhesive Protein Fused with the BC Domain of Protein A is a Functional Linker Material that Efficiently Immobilizes Antibodies onto Diverse Surfaces

A Mussel Adhesive Protein Fused with the BC Domain of Protein A is a Functional Linker Material that Efficiently Immobilizes Antibodies onto Diverse Surfaces

Plasmonic Sensors for Disease Detection - A Review

Surface functionalization allowing repetitive use of optical sensors for real‐time detection of antibody‐bacteria interaction

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