Label-free liquid crystal-based detection of As(III) ions using ssDNA as a recognition probe

Nguyen, Duy Khiem; Jang, Chang‐Hyun

doi:10.1016/j.microc.2020.104834

Cited by 55 publications

(24 citation statements)

References 49 publications

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“…Previous studies have demonstrated that CTAB could be used to regulate the orientation of the LCs at the LC- aqueous interface [ 42 , 43 ]. Therefore, the minimum concentration of CTAB sufficient enough to trigger the homeotropic anchoring of the LCs was first determined.…”

Section: Resultsmentioning

confidence: 99%

“…These results indicated that the LC molecules undergo an orientational transition from homeotropic to planar/tilted orientation with the increase in the aptamer concentration. This is due to the electrostatic interactions between the cationic head groups of CTAB and the anionic phosphate groups of the DNA aptamer disrupting the homeotropic alignment [ 41 , 42 , 43 ]. Moreover, the average GI values of the corresponding optical images gradually increased with increasing aptamer concentration and reached a maximum value at the aptamer concentration of 200 nM ( Figure 2 g).…”

Section: Resultsmentioning

confidence: 99%

“…OTS-coated glass slides and optical cells were prepared as previously described [ 38 , 43 ]. The detailed procedures are provided in the Supplementary Material .…”

Section: Methodsmentioning

confidence: 99%

“…Recently, LC-based biosensors using surfactant-decorated aqueous/LC interface have been widely developed for monitoring various biochemical events, such as enzymatic reactions [ 38 , 39 ], biomolecular interactions [ 40 , 41 ], and heavy metal ions [ 42 , 43 ]. In this paper, we constructed an LC-based sensing platform to detect malathion, a pesticide toxic to humans, in aqueous solutions.…”

Section: Introductionmentioning

confidence: 99%

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A Cationic Surfactant-Decorated Liquid Crystal-Based Aptasensor for Label-Free Detection of Malathion Pesticides in Environmental Samples

Nguyen

Jang

2021

Biosensors

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We report a liquid crystal (LC)-based aptasensor for the detection of malathion using a cationic surfactant-decorated LC interface. In this method, LCs displayed dark optical images when in contact with aqueous cetyltrimethylammonium bromide (CTAB) solution due to the formation of a self-assembled CTAB monolayer at the aqueous/LC interface, which induced the homeotropic orientation of LCs. With the addition of malathion aptamer, the homeotropic orientation of LCs changed to a planar one due to the interactions between CTAB and the aptamer, resulting in a bright optical image. In the presence of malathion, the formation of aptamer-malathion complexes caused a conformational change of the aptamers, thereby weakening the interactions between CTAB and the aptamers. Therefore, CTAB is free to induce a homeotropic ordering of the LCs, which corresponds to a dark optical image. The developed sensor exhibited high specificity for malathion determination and a low detection limit of 0.465 nM was achieved. Moreover, the proposed biosensor was successfully applied to detect malathion in tap water, river water, and apple samples. The proposed LC-based aptasensor is a simple, rapid, and convenient platform for label-free monitoring of malathion in environmental samples.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…OTS-coated glass slides and optical cells were prepared as previously described [ 38 , 43 ]. The detailed procedures are provided in the Supplementary Material .…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Cationic Surfactant-Decorated Liquid Crystal-Based Aptasensor for Label-Free Detection of Malathion Pesticides in Environmental Samples

Nguyen

Jang

2021

Biosensors

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“… Electrochemical (cyclic voltammetry and differential pulse voltammetry) 0.003 × 10 −3 0.01–10 [ 119 ] Label-free LC-based aptasensor, specific arsenic aptamer, CTAB as a cationic surfactant, micro-glass slides, OTS, 5CB. Optical (polarized light microscope) 400 - [ 120 ] Hairpin DNA restricted in PDANS, induction of SDA process by polymerase and endonuclease, ITO electrode in the presence of Ru (phen) 3 2+ as ECL probe. Optical (Electrochemiluminescence) 1.2 × 10 −3 - [ 121 ] Whole-cell (bacterial) biosensor based on arsenical-resistant genes, reporter gene carrying FGE, and sulfatase, which reacts with 4-MUS.…”

Section: Biosensors Designing Based On Nanotechnology Approach To Heamentioning

confidence: 99%

Recent Advances in Nanotechnology-Based Biosensors Development for Detection of Arsenic, Lead, Mercury, and Cadmium

Maghsoudi¹,

Hassani²,

Mirnia³

et al. 2021

IJN

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Heavy metals cause considerable environmental pollution due to their extent and non-degradability in the environment. Analysis and trace levels of arsenic, lead, mercury, and cadmium as the most toxic heavy metals show that they can cause various hazards in humans’ health. To achieve rapid, high-sensitivity methods for analyzing ultra-trace amounts of heavy metals in different environmental and biological samples, novel biosensors have been designed with the participation of strategies applied in nanotechnology. This review attempted to investigate the novel, sensitive, efficient, cost-benefit, point of care, and user-friendly biosensors designed to detect these heavy metals based on functional mechanisms. The study’s search strategies included examining the primary databases from 2015 onwards and various keywords focusing on heavy metal biosensors’ performance and toxicity mechanisms. The use of aptamers and whole cells as two important bio-functional nanomaterials is remarkable in heavy metal diagnostic biosensors’ bioreceptor design. The application of hybridized nanomaterials containing a specific physicochemical function in the presence of a suitable transducer can improve the sensing performance to achieve an integrated detection system. Our study showed that in addition to both labeled and label-free detection strategies, a wide range of nanoparticles and nanocomposites were used to modify the biosensor surface platform in the detection of heavy metals. The detection limit and linear dynamic range as an essential characteristic of superior biosensors for the primary toxic metals are studied. Furthermore, the perspectives and challenges facing the design of heavy metal biosensors are outlined. The development of novel biosensors and the application of nanotechnology, especially in real samples, face challenges such as the capability to simultaneously detect multiple heavy metals, the interference process in complex matrices, the efficiency and stability of nanomaterials implemented in various laboratory conditions.

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Design and Applications of Liquid Crystal Biophotonic Sensors for Ion Detection

2022

Advanced Photonics Research

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Liquid crystals (LCs) are nanophotonic materials which can interact with light in the molecular scale. While the LCs are widely used in various displays, last decade has witnessed emerging applications of the LCs for nondisplays. Based on the unique stimuli–responsive property and optical property, the applications of the LCs in the field of sensors are widely explored. In addition, ions are widely distributed in nature and bionts, playing critical roles in many physiological activities and ecological cycles. Appropriate amount of ions is beneficial to the human body, while the excess ions can cause irreversible damages. As a result, a variety of LC sensors are proposed to detect the ion concentration in both body fluids and water samples. This article reviews the design principles, applications, and existing problems of the LC ion sensors. The geometries, alignments, and optical signals of the sensors, which can guide the design of the devices, are first discussed. Afterward, two types of LC ion sensors aiming at detecting metal ions and nonmetallic ions, respectively, are illustrated. Finally, the current challenges and potential development directions of the LC ion sensors are introduced briefly.

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Label-free liquid crystal-based detection of As(III) ions using ssDNA as a recognition probe

Cited by 55 publications

References 49 publications

A Cationic Surfactant-Decorated Liquid Crystal-Based Aptasensor for Label-Free Detection of Malathion Pesticides in Environmental Samples

A Cationic Surfactant-Decorated Liquid Crystal-Based Aptasensor for Label-Free Detection of Malathion Pesticides in Environmental Samples

Recent Advances in Nanotechnology-Based Biosensors Development for Detection of Arsenic, Lead, Mercury, and Cadmium

Design and Applications of Liquid Crystal Biophotonic Sensors for Ion Detection

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