Label-Free LC-MS/MS Analysis Reveals Different Proteomic Profiles between Egg Yolks of Silky Fowl and Ordinary Chickens

Abstract

The proteomic profiles of Silky fowl egg yolk (SFEY) and Leghorn egg yolk (LEY) were analyzed by bottom-up label-free liquid chromatography–tandem mass spectrometry (LC-MS/MS). From a total of 186 identified proteins, 26 proteins were found significantly differentially abundant between two yolks, of which, 19 were up-regulated and 7 were down-regulated in SFEY, particularly, vitelline membrane outer layer protein 1, transthyretin and ovoinhibitor were up-regulated by 26, 25, and 16 times, respectively. In addition, there were 57 and 6 unique proteins in SFEY and LEY, respectively. Gene Ontology (GO) revealed SFEY contained relatively more abundant protease inhibitors and coagulation-related proteins. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed differentially abundant proteins in SFEY may be actively involved in the regulation of the neuroactive ligand–receptor interaction pathway. This study provides a theoretical basis for the understanding of proteomic and biological differences between these two yolks and can guide for further exploration of nutritional and biomedical use of Silky fowl egg.

Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom

“…We detected the presence of a diverse range of co-isolated proteins, including components of the avian immune system and proteins with structural functions or associated with lipid metabolism. To our knowledge, this is the first report of a full characterization of IgY extracts obtained by ammonium sulfate precipitation (Mann and Mann 2008;Nilsson et al 2008;Wang et al 2020;Wu et al 2022). For example, previous works only reported the co-isolation of a protein band, identified as a vitellogenin-2 fragment, in egg yolk extracts (Klimentzou et al 2006).…”