Label-free kinetic study of biomolecular interactions by white light reflectance spectroscopy

Zavali, Maria; Petrou, Panagiota; Kakabakos, Sotirios; Κιτσαρά, Μαρία; Raptis, Ioannis; Beltsios, K.; Μισιακός, Κωνσταντίνος

doi:10.1049/mnl:20065019

Cited by 27 publications

(22 citation statements)

References 6 publications

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“…The results show that the prepared glycoclusters can bind and partially protect ampicillin from degradation. 27,28 to monitor and quantify GNL interaction with 3 and WGA interaction with 5. The lectins were deposited on a surface in a fluidic cell (see Experimental) and formed a stable layer upon which solutions of 3 (or 5) were introduced.…”

Section: Resultsmentioning

confidence: 99%

“…The solutions were left to stand for 12 h at 4 o C before deposition on the interferometric WLRS setup, previously described in detail. 27,28 Briefly, four-inch silicon wafers with a thermally grown 300 nm thick SiO 2 film as the interferometric layer were used, upon which a thin novolac resin film (AZ-5214, AZ-EM materials) was deposited by spin coating and post-baked at a high temperature (180 o C, 1 h) to improve coating efficiency (50 to 100 Å). The coated wafer was placed in the incubation-fluidic cell and the protein solutions were channeled in at 60 µL/min and ∆λ max was monitored until a plateau was reached.…”

Section: Coupling Reactions Octakis-{6-deoxy-6-s-[6-(α-d-mannopyranosmentioning

confidence: 99%

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Synthesis of cyclodextrin derivatives with monosaccharides and their binding with ampicillin and selected lectins

Lampropoulou¹,

Μισιακός²,

Paravatou³

et al. 2015

Arkivoc

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β-and γ-Cyclodextrins connected on their narrow side via 6 bond-linkers with monosaccharides (Man, GlcNAc) suitable for bacterial lectin recognition, along with reference mono-analogues were prepared. The resulting compounds: (a) posses a functional cavity forming inclusion complexes with a model guest molecule and with the antibiotic ampicillin; (b) inhibit by more than 30% the hydrolysis of ampicillin in the presence of the enzyme β-lactamase; (c) interact stronger with surface immobilized lectins than the reference mono-analogues. The present work suggests that combining the lectin recognition property of the compounds with their ability to encapsulate antibiotics could be used to target and kill bacteria.

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Section: Resultsmentioning

confidence: 99%

Section: Coupling Reactions Octakis-{6-deoxy-6-s-[6-(α-d-mannopyranosmentioning

confidence: 99%

Synthesis of cyclodextrin derivatives with monosaccharides and their binding with ampicillin and selected lectins

Lampropoulou¹,

Μισιακός²,

Paravatou³

et al. 2015

Arkivoc

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“…WLRS offers great precision for determination of the thickness of a transparent spacer layer on a reflecting surface [63][64][65][66][67][68][69]. A broadband light source is focused onto the surface and reflected light is collected.…”

Section: White Light Reflectance Spectroscopymentioning

confidence: 99%

Spectral Self-Interference Fluorescence Microscopy to Study Conformation of Biomolecules with Nanometer Accuracy

Zhang¹,

Spuhler²,

Freedman³

et al. 2013

Nanoscale Spectroscopy With Applications

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“…The spectral shift as a function of time was used to determine the binding reaction. Zavali et al (19,20 ) have described a system that uses white light reflectance spectroscopy to demonstrate IgG binding to anti-IgG. The detection of a 150 pmol/L concentration was shown within 1 minute.…”

Section: Optical Immunosensor Systemsmentioning

confidence: 99%

Discerning Trends in Multiplex Immunoassay Technology with Potential for Resource-Limited Settings

Gordon

Michel

2012

Clinical Chemistry

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BACKGROUND In the search for more powerful tools for diagnoses of endemic diseases in resource-limited settings, we have been analyzing technologies with potential applicability. Increasingly, the process focuses on readily accessible bodily fluids combined with increasingly powerful multiplex capabilities to unambiguously diagnose a condition without resorting to reliance on a sophisticated reference laboratory. Although these technological advances may well have important implications for the sensitive and specific detection of disease, to date their clinical utility has not been demonstrated, especially in resource-limited settings. Furthermore, many emerging technological developments are in fields of physics or engineering, which are not readily available to or intelligible to clinicians or clinical laboratory scientists. CONTENT This review provides a look at technology trends that could have applicability to high-sensitivity multiplexed immunoassays in resource-limited settings. Various technologies are explained and assessed according to potential for reaching relevant limits of cost, sensitivity, and multiplex capability. Frequently, such work is reported in technical journals not normally read by clinical scientists, and the authors make enthusiastic claims for the potential of their technology while ignoring potential pitfalls. Thus it is important to draw attention to technical hurdles that authors may not be publicizing. SUMMARY Immunochromatographic assays, optical methods including those involving waveguides, electrochemical methods, magnetorestrictive methods, and field-effect transistor methods based on nanotubes, nanowires, and nanoribbons reveal possibilities as next-generation technologies.

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Label-free kinetic study of biomolecular interactions by white light reflectance spectroscopy

Cited by 27 publications

References 6 publications

Synthesis of cyclodextrin derivatives with monosaccharides and their binding with ampicillin and selected lectins

Synthesis of cyclodextrin derivatives with monosaccharides and their binding with ampicillin and selected lectins

Spectral Self-Interference Fluorescence Microscopy to Study Conformation of Biomolecules with Nanometer Accuracy

Discerning Trends in Multiplex Immunoassay Technology with Potential for Resource-Limited Settings

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