Label-free impedimetric aptasensor for lysozyme detection based on carbon nanotube-modified screen-printed electrodes

Rohrbach, Falk; Karadeniz, Hakan; Erdem, Arzum; Famulok, Michael; Mayer, Günter

doi:10.1016/j.ab.2011.11.034

Cited by 115 publications

(60 citation statements)

References 35 publications

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“…The selectivity of the electrochemical aptasensors was proven by challenging them with excess quantities of other proteins (e.g., bovine serum albumin, thrombin, immunoglobulin G, hemoglobin, myoglobin, casein, cytochrome C) tested either in individual solutions or in mixtures with lysozyme [44,46,61,74,76,77,79,82,84,[89][90][91]. Some authors went further and proved also the selectivity of the lysozyme aptamer sequence by performing measurements with an identical sensor in which the aptamer was replaced by a scrambled DNA sequence [81,82,87].…”

Section: Evaluation Of Selectivity Reproducibility and Storage Stabimentioning

confidence: 99%

“…Some authors went further and proved also the selectivity of the lysozyme aptamer sequence by performing measurements with an identical sensor in which the aptamer was replaced by a scrambled DNA sequence [81,82,87]. For example, the current decrease in the adenine oxidation signal, taken as the analytical signal in the aptasensor developed by Rodriguez and Rivas [81], was only 2.7% with a scrambled sequence as compared to 87.6% for the lysozyme aptamer [81].…”

Section: Evaluation Of Selectivity Reproducibility and Storage Stabimentioning

confidence: 99%

“…Rohrbach et al modified screen-printed graphite electrodes with multi-walled carbon nanotubes (MWCNTs) containing about 5% carboxylic acid groups [82]. Another strategy relied on covering a Au electrode with hollow TiO 2 spheres and a film of polyacrylic acid [75], where polyacrylic acid provided the carboxyl groups for aptamer immobilization, while the hollow TiO 2 spheres enabled increased surface area for higher aptamer loading and improved electrical characteristics.…”

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confidence: 99%

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Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

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Protein analysis and quantification are required daily by thousands of laboratories worldwide for activities ranging from protein characterization to clinical diagnostics. Multiple factors have to be considered when selecting the best detection and quantification assay, including the amount of protein available, its concentration, the presence of interfering molecules, as well as costs and rapidity. This is also the case for lysozyme, a 14.3-kDa protein ubiquitously present in many organisms, that has been identified with a variety of functions: antibacterial activity, a biomarker of several serious medical conditions, a potential allergen in foods or a model of amyloid-type protein aggregation. Since the design of the first lysozyme aptamer in 2001, lysozyme became one of the most intensively-investigated biological target analytes for the design of novel biosensing concepts, particularly with regards to electrochemical aptasensors. In this review, we discuss the state of the art of aptamer-based electrochemical sensing of lysozyme, with emphasis on sensing in serum and real samples.

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Section: Evaluation Of Selectivity Reproducibility and Storage Stabimentioning

confidence: 99%

Section: Evaluation Of Selectivity Reproducibility and Storage Stabimentioning

confidence: 99%

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confidence: 99%

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Aptamer-Based Electrochemical Sensing of Lysozyme

Vasilescu

Wang

et al. 2016

Chemosensors

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“…This technique was one of the first used for electrochemical transduction of the aptamer-ligand recognition event for small molecules [27], where the associated conformational change can be too small to cause a significant variation in a physical property of appropriate labels such as the case of antibiotics [28,29]. But it can also be used for the detection of large molecules [30][31][32] or whole cells [33]. The analytical signal, the electron transfer resistance (R et ) of a redox probe, is related to the amount of protein bound to the aptamer immobilized on the electrode surface, which correlates with the concentration of proteins present in the sample.…”

Section: Introductionmentioning

confidence: 99%

A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins

Queirós

de‐los‐Santos‐Álvarez

Noronha

et al. 2013

Sensors and Actuators B: Chemical

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a b s t r a c tA label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins (OMPs) was developed. Two single stranded DNA sequences were tested as recognition elements and compared. The aptamer capture probes were immobilized, with and without 6-mercapto-1-hexanol (MCH) on a gold electrode. Each step of the modification process was characterized by Faradaic impedance spectroscopy (FIS). A linear relationship between the electron-transfer resistance (R et ) and E. coli OMPs concentration was demonstrated in a dynamic detection range of 1 × 10 −7 -2 × 10 −6 M. Moreover, the aptasensor showed selectivity despite the presence of other possible water contaminates and could be regenerated under low pH condition. The developed biosensor shows great potential to be incorporated in a biochip and used for in situ detection of E. coli OMPs in water samples.

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“…Phage production is possible in an easier, faster, and cheaper, which makes them promising tools in biosensing Balasubramanian et al, 2007;Zeikus and Johnson, 1991;Yang and Bashir, 2008). They can also be immobilized onto transducing devices which makes detection more rapid biosensor platforms being developed for electrochemical analysis by impedometric-based detection (Zourob, 2010;Rohrbach et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage based self-assembled monolayer (SAM) on gold surface used for detection of Escherichia coli by electrochemical analysis

Singh¹,

Jain²,

Dahiya³

2015

Afr. J. Microbiol. Res.

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Bacteriophages (or phages) are parasites that infect specific bacteria. This host-selectivity can be useful to identify bacterial contaminants in food, water, environment etc. In the present study, phage was isolated from stagnant water and cultivated by overlay method against host bacteria that is E. coli. Phage titer was calculated to be 10 7 pfu/ml using 10-fold dilutions. Plaque reaction activity was observed within 4 to 6 h against host bacteria by spot test. Morphological identification of phage by transmission electron microscopy (TEM) using uranyl acetate staining revealed about 78 nanometers (nm) in wide phage capsid and tail length (527 nm). The isolated phage was classified into order Caudovirales since it possessed a long non-contractile tail and icosahedral capsid head, thus is a member of the family Siphoviridae. Also, the phage identified followed a lytic life cycle since plaque reaction activity was observed within 4-6 h against host bacteria. Gold immuno-functionalization using self-assembled monolayers (SAM) has been widely used for the detection of small targets, but there are limited reports available describing the detection systems for bacteria by using phages. Thus, in order to develop a suitable detection system for identification of specific bacteria, it is suitable to exploit the close association and selectivity between bacteria and bacteriophage. Bacteriophages were immobilized onto gold surface by SAM using a stable acyl amino ester intermediate generated by 1-ethyl-3(3-dimethylaminoproply) carbodiimide (EDC) and N-hydrosuccinimide (NHS) to condense the bacteriophage. Fourier transformation infrared (FTIR) microscopy presence of different functional groups present in each layer formation. Bacteriophage immobilization over the gold surface was verified through by scanning electron microscope (SEM). Electrochemical analysis was performed for a rapid and specific detection of E. coli cell. The present bio-sensing system comprises of quick and specific detection of host bacteria and possesses a very low detection limit (10 4 cfu/ml). We propose phages utilization as a bio-component in biosensor development for bacteria capture.

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Label-free impedimetric aptasensor for lysozyme detection based on carbon nanotube-modified screen-printed electrodes

Cited by 115 publications

References 35 publications

Aptamer-Based Electrochemical Sensing of Lysozyme

Aptamer-Based Electrochemical Sensing of Lysozyme

A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins

Bacteriophage based self-assembled monolayer (SAM) on gold surface used for detection of Escherichia coli by electrochemical analysis

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