Label-free immunosensor based on one-step electrodeposition of chitosan-gold nanoparticles biocompatible film on Au microelectrode for determination of aflatoxin B1 in maize

Ma, Haihua; Sun, Junqi; Zhang, Yuan; Bian, Chao; Xia, Shanhong; Tong, Zhen

doi:10.1016/j.bios.2016.01.063

Cited by 104 publications

(36 citation statements)

References 42 publications

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“…In a very recent research report, a nanocomposite of chitosan‐AuNPs was electrodeposited on a gold microelectrode surface to develop a label free impedimetric immunosensor of AFB1 in maize samples. The authors demonstrated that this nanocomposite offered a good conductivity, excellent biocompatibility and stability for antibody immobilization . The incorporation of nanostructures improves the analytical performance of the electrochemical biosensing methods owing to their large surface area, ease of preparation and bioconjugation and excellent electrochemical activity.…”

Section: Applications Of Electrochemical Immunosensors For Mycotoxinmentioning

confidence: 85%

An Overview of Recent Electrochemical Immunosensing Strategies for Mycotoxins Detection

et al. 2016

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Electrochemical immunosensors are affinity ligand based biosesnors based on solid state devices in which immunochemical reactions are coupled to a transducer surface to generate the output electrochemical signal. The concept of immunosensor methodology is similar to conventional ELISA based immunoassays; however, in contrast to immunoassays, modern transducer technology permits the highly sensitive quantification of the immune complex in divers ways. Mycotoxins are naturally occurring toxic secondary metabolites produced by fungi contaminating food and feed and are responsible for mycotoxicosis with symptoms of intoxication causing substantial effects on animal and human health. Based on the adverse effect, it has been very crucial to develop ultrasensitive sensing methodologies to ensure food safety and prevent risks in agro‐food and environmental sector. For these purposes, many electrochemical immunosensors based on different analyzing techniques have been reported for mycotoxins monitoring. In this review paper, we provided a general overview on the practical aspects of electrochemical imunosensor designs such as assay format, choice of the transducer surface, immobilization methodologies, signal generating probe and type of output electrochemical signal. In doing so, we also reviewed the recent development in electrochemical immunosensor applications for mycotoxin analysis.

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Section: Applications Of Electrochemical Immunosensors For Mycotoxinmentioning

confidence: 85%

An Overview of Recent Electrochemical Immunosensing Strategies for Mycotoxins Detection

et al. 2016

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“…Due to the high toxicity and carcinogenicity of aflatoxins, many countries have set up strict maximum permissible limits for AFB 1 or total aflatoxins (aflatoxin B 1 , B 2 , G 1 and G 2 ) in food and agriproducts. 4 Accurate and sensitive determination of aflatoxins became an important requirement to meet food safety concerns and official legislation.…”

Section: Introductionmentioning

confidence: 99%

“…For example, Zhang's group reported that immunosensor for detection of AFB 1 by gold nanoparticles embedded in chitosan film used one-step electrodeposion. 4 Hu's group developed a non-fouling polymer brush-based fluorescent competitive immunoassay microarray to detect AFB 1 . 9 Xu's group developed a label-free electrochemiluminescent immunoassay based on magnetic nanoarchitecture for sensitive detection of AFB 1 .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Immunosensor for Aflatoxin B<sub>1</sub> Based on Polyaniline/Graphene Nanohybrids Decorated with Au Nanoparticle

et al. 2017

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A novel method for preparing polyaniline/graphene (PANI/GN) nanocomposites was demonstrated by liquid-liquid interface polymerization method. Then the gold nanoparticles (Au NPs) were uniformly decorated on the PANI/GN surface to form gold/polyaniline/graphene (Au/PANI/GN) nanohybrids. The Au/PANI/GN nanohybrids film showed good electron transfer ability, which ensured high sensitivity to detect AFB 1 in a range from 0.05 to 25 ng/mL with a detection limit of 0.034 ng/mL obtained at 3σ (n = 10). The proposed immunosensor provided a simple tool for AFB 1 detection.

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“…Various electrochemical immunosensors using nanoparticles have been reported for these compounds [56]. Table 1 summarizes the analytical characteristics of recent immunosensors constructed for aflatoxin B1 (AFB1) [57][58][59][60]. A relevant example is the strategy involving a Chit-AuNPs nanocomposite electrodeposited onto the surface of a disk-ring gold microelectrode to prepare a label-free impedimetric immunosensor for AFB1 in wheat samples [57].…”

Section: Introductionmentioning

confidence: 99%

“…Table 1 summarizes the analytical characteristics of recent immunosensors constructed for aflatoxin B1 (AFB1) [57][58][59][60]. A relevant example is the strategy involving a Chit-AuNPs nanocomposite electrodeposited onto the surface of a disk-ring gold microelectrode to prepare a label-free impedimetric immunosensor for AFB1 in wheat samples [57]. The specific antibody was immobilized onto the modified electrode and the sample was incubated for 15 min followed by CV measurement in the presence of [Fe(CN) 6 ] 3−/4− .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Affinity Biosensors in Food Safety

2017

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Safety and quality are key issues of today's food industry. Since the food chain is becoming more and more complex, powerful analytical methods are required to verify the performance of food safety and quality systems. Indeed, such methods require high sensitivity, selectivity, ability for rapid implementation and capability of automatic screening. Electroanalytical chemistry has, for decades, played a relevant role in food safety and quality assessment, taking more and more significance over time in the solution of analytical problems. At present, the implementation of electrochemical methods in the food is evident. This is in a large part due to the relevant results obtained by combining the attractive advantages of electrochemical transduction strategies (in terms of relatively simple hardware, versatility, interface with automatic logging and feasibility of application outside the laboratory environment) with those from biosensors technology. Important examples of enzyme electrochemical biosensors are those dedicated to the determination of glucose, alcohol or cholesterol are important examples. In addition, other types of different electrochemical biosensing approaches have emerged strongly in the last years. Among these, the strategies involving affinity interactions have been shown to possess a large number of applications. Therefore, electrochemical immunosensors and DNA-based biosensors have been widely used to determine major and minor components in foodstuffs, providing sufficient data to evaluate food freshness, the quality of raw materials, or the origin of samples, as well as to determine a variety of compounds at trace levels related to food safety such as micotoxins, allergens, drugs residues or pathogen microorganisms. This review discusses some critical examples of the latest advances in this area, pointing out relevant methodologies related to the measurement techniques, including the use of nanostructured electrodes and strategies for signal amplification.

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Label-free immunosensor based on one-step electrodeposition of chitosan-gold nanoparticles biocompatible film on Au microelectrode for determination of aflatoxin B1 in maize

Cited by 104 publications

References 42 publications

An Overview of Recent Electrochemical Immunosensing Strategies for Mycotoxins Detection

An Overview of Recent Electrochemical Immunosensing Strategies for Mycotoxins Detection

Electrochemical Immunosensor for Aflatoxin B<sub>1</sub> Based on Polyaniline/Graphene Nanohybrids Decorated with Au Nanoparticle

Electrochemical Affinity Biosensors in Food Safety

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