Label-free imaging of fibroblast membrane interfaces and protein signatures with vibrational infrared photothermal and phase signals: publisher’s note

Abstract

This publisher’s notes amends the funding of [Biomed. Opt. Express 12, 303 (2021) 10.1364/BOE.411888].

Mid-infrared Photothermal Imaging: Instrument and Life Science Applications

“…The photothermal decay induces a phase shift between the demodulated AC signal and the reference clock in the lock-in amplifier. By reading the phase channel signal from the lock-in amplifier, this advanced approach offers improved sensitivity to resolve nanostructures in aqueous environments. , Notably, due to a low duty cycle of MIP signal, the lock-in amplifier is not an ideal strategy because the demodulated output includes extra noise from the signal-off status. Besides, the lock-in detection method requires a few cycles of signals and demodulates only the fundamental component of the photothermal signal, which limits the imaging speed and sensitivity.…”

Label-free mid-infrared photothermal live-cell imaging beyond video rate

“…In addition, imaging with MIR light holds a possibility of obtaining information that Raman spectra have not revealed until now. MIR spectra provide rich information on biomolecules that dominantly exist in a cell, such as proteins and water, more specifically, e.g., the secondary structure of proteins via the amide band [15][16][17] and the state of water molecules via the OH band 18 .…”