Label-Free Highly Sensitive Detection of Proteins in Aqueous Solutions Using Surface-Enhanced Raman Scattering

Han, Xiao; Huang, Genin Gary; Zhao, Bing; Ozaki, Yukihiro

doi:10.1021/ac900395x

Cited by 203 publications

(202 citation statements)

References 38 publications

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“…[15 -18,22 -27] However, to our best knowledge, a quantitative SERS study of protein Class II in a broad concentration range has not been reported except for our recent study. [14] For the quantitative SERS studies of protein Class II, an experimental protocol for routine analyses as well as professional investigation is needed.…”

Section: Introductionmentioning

confidence: 99%

Experimental parameters for the SERS of nitrate ion for label‐free semi‐quantitative detection of proteins and mechanism for proteins to form SERS hot sites: a SERS study

Zhou

Huang

Kato

et al. 2011

J Raman Spectroscopy

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We have explored the effects of the experimental parameters on the surface-enhanced Raman scattering (SERS) intensities of NO 3 − and proteins observed by a heat-induced SERS method developed by our group. The results have shown that a strong SERS signal can be obtained at pH 4.0, using an Ag colloid prepared with the reduction time of 15 min (the average size of Ag nanoparticle is 56.5 nm) dilution prepared Ag colloid by a factor of 2 by use of a 5 mM citrate buffer, using 6 mM NaNO 3 and drying the sample at 100 • C, respectively. Based on the results, two possible mechanisms for proteins to form SERS hot sites during the sample preparations are proposed. A semi-quantitative SERS detection of ribonuclease B has been investigated. Also, NaNO 2 , Mg (NO 3 ) 2 , MgSO 4 and Na 2 SO 4 have been found to be suitable for the heat-induced SERS method. Importantly, samples prepared by the heat-induced SERS method are so stable that these samples can be used as a standard and transferred to different laboratories for direct comparison. Namely, it can overcome uncontrollable aggregation of Ag colloids in a solution sample. All these advantages and the simplicity of experimental setup have demonstrated that the heat-induced SERS method using NaNO 3 as an electrolyte is very promising for label-free routine and quantitative detection of proteins.

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Section: Introductionmentioning

confidence: 99%

Experimental parameters for the SERS of nitrate ion for label‐free semi‐quantitative detection of proteins and mechanism for proteins to form SERS hot sites: a SERS study

Zhou

Huang

Kato

et al. 2011

J Raman Spectroscopy

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“…This protocol allows for simple, sensitive, and reproducible label-free detection proteins with LODs as low as 50 ng/ml [214].…”

Section: Sers Of Proteinsmentioning

confidence: 99%

Fundamentals and applications of SERS-based bioanalytical sensing

et al. 2017

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Abstract:Plasmonics is an emerging field that examines the interaction between light and metallic nanostructures at the metal-dielectric interface. Surface-enhanced Raman scattering (SERS) is a powerful analytical technique that uses plasmonics to obtain detailed chemical information of molecules or molecular assemblies adsorbed or attached to nanostructured metallic surfaces. For bioanalytical applications, these surfaces are engineered to optimize for high enhancement factors and molecular specificity. In this review we focus on the fabrication of SERS substrates and their use for bioanalytical applications. We review the fundamental mechanisms of SERS and parameters governing SERS enhancement. We also discuss developments in the field of novel SERS substrates. This includes the use of different materials, sizes, shapes, and architectures to achieve high sensitivity and specificity as well as tunability or flexibility. Different fundamental approaches are discussed, such as label-free and functional assays. In addition, we highlight recent relevant advances for bioanalytical SERS applied to small molecules, proteins, DNA, and biologically relevant nanoparticles. Subsequently, we discuss the importance of data analysis and signal detection schemes to achieve smaller instruments with low cost for SERS-based point-of-care technology developments. Finally, we review the main advantages and challenges of SERS-based biosensing and provide a brief outlook.

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“…[14][15][16][17] There are two types of protein research methods based on SERS, label-based and label-free methods. Label-based detection is an indirect detection strategy that employs a Raman-active molecule with high Raman scattering crosssections, which can provide great SERS enhancement.…”

Section: Introductionmentioning

confidence: 99%

Avidin Induced Silver Aggregation for SERS-based Bioassay

Sa¹,

Chen²,

Jung

2012

Bulletin of the Korean Chemical Society

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We developed a simple and effective method for the SERS-based detection of protein-small molecule complexes and label-free proteins using avidin-induced silver aggregation. Upon excitation with light of the appropriate wavelength (633 and 532 nm), the aggregated silver nanoparticles generate a strong electric field that couples with the resonance of the molecules (atto610 and cytochrome c), increasing the characteristic signals of these molecules and resulting in sensitive detection. The detection limit of biotin with the proposed method is as low as 48 ng/mL. The most important aspect of this method is the induction of silver aggregation by a protein (avidin), which makes the silver more biocompatible. This technique is very useful for the detection of protein-small molecule complexes.

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Label-Free Highly Sensitive Detection of Proteins in Aqueous Solutions Using Surface-Enhanced Raman Scattering

Cited by 203 publications

References 38 publications

Experimental parameters for the SERS of nitrate ion for label‐free semi‐quantitative detection of proteins and mechanism for proteins to form SERS hot sites: a SERS study

Experimental parameters for the SERS of nitrate ion for label‐free semi‐quantitative detection of proteins and mechanism for proteins to form SERS hot sites: a SERS study

Fundamentals and applications of SERS-based bioanalytical sensing

Avidin Induced Silver Aggregation for SERS-based Bioassay

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