Label-free fluorometric detection of chymotrypsin activity using graphene oxide/nucleic-acid-stabilized silver nanoclusters hybrid materials

Li, Shuangqin; Yuewei, Fu,; Ma, Xuejuan; Zhang, Yaodong

doi:10.1016/j.bios.2016.08.029

Cited by 24 publications

(3 citation statements)

References 42 publications

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“…Phenylmethanesulfonyl fluoride (PMSF), an inhibitor of CHT, can inhibit serine proteases by sulfonating serine residues. 41 As shown in Fig. 1E, PMSF could induce an obvious decrease in the fluorescence intensity, and the fluorescence intensity showed a good linear relationship with the concentration of PMSF (Fig.…”

Section: Resultsmentioning

confidence: 70%

Activatable endoplasmic reticulum-targeted NIR fluorescent probe with a large Stokes shift for detecting and imaging chymotrypsin

Lan

Tian

et al. 2022

Analyst

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Section: Resultsmentioning

confidence: 70%

Activatable endoplasmic reticulum-targeted NIR fluorescent probe with a large Stokes shift for detecting and imaging chymotrypsin

Lan

Tian

et al. 2022

Analyst

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“…The first selection, containing the cytosine oligonucleotide dC 12 , could bind with Ag + followed by NaBH 4 reduction to prepare DNA-AgNCs. Some studies have used dC 12 as an effective basic substance to composite DNA-AgNCs [ 28 , 29 , 30 ]. The second selection, which included a consecutive adenine (Poly A) and its binding to the AuNPs, quenched the fluorescence of AgNCs using FRET.…”

Section: Resultsmentioning

confidence: 99%

A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of Salmonella Typhimurium

Yang

Pang

et al. 2022

Foods

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Salmonella Typhimurium (S. Typhimurium) is a globally distributed foodborne pathogen, which can lead to outbreaks of foodborne infectious diseases. It is essential to guarantee food safety by timely and correct detection of S. Typhimurium. In this investigation, an original fluorescence aptasensor was constructed to detect S. Typhimurium rapidly and sensitively. Through the coupling of magnetic beads, aptamer, and gold nanoparticles (AuNPs), a fluorescence quenching system with a “sandwich structure” was established. The aptamer acted as a link, and its specific binding to S. Typhimurium could release AuNPs from the system. Meanwhile, fluorescent DNA-stabilized silver nanoclusters (DNA-AgNCs) were synthesized. The fluorescence intensity changes caused by the fluorescence resonance energy transfer between DNA-AgNCs and AuNPs were utilized to detect S. Typhimurium. The purposed aptasensor exhibited high selectivity and sensitivity with a linear response to S. Typhimurium, ranging from 3.7 × 102 to 3.7 × 105 cfu/mL. The limit of detection (LOD) was estimated to be 98 cfu/mL within 2 h 10 min. In addition, this method showed excellent application for detection of S. Typhimurium in artificially contaminated milk, with LOD reaching 3.4 × 102 cfu/mL. Therefore, the developed fluorescence aptasensor has great potential to identify S. Typhimurium in foodstuffs.

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“…Other macromolecules, such as BSA and Tryspin, have no interference with the detection, which is mainly attributed to the special performance of rGO. As reported, rGO has a strong adsorption for single-stranded DNA and a poor adsorption for double-stranded DNA. − However, many biological macromolecules in the human body are double-stranded DNA, so they cannot be efficiently adsorbed on rGO. Meanwhile, the level of Hcy is much lower than that of GSH, so it does not significantly interfere with GSH detection.…”

Section: Feasibility Of Gsh Assay and The Possible Mechanism Of “Crow...mentioning

confidence: 99%

Endogenous Cys-Assisted GSH@AgNCs-rGO Nanoprobe for Real-Time Monitoring of Dynamic Change in GSH Levels Regulated by Natural Drug

et al. 2019

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Glutathione (GSH) levels are closely related to the homeostasis of redox state which directly affects human disease occurrence by regulating cell apoptosis. Hence, real-time monitoring of dynamic changes in intracellular GSH levels is urgently needed for disease early diagnosis and evaluation of therapy efficiency. In this study, an endogenous cysteine (Cys)-assisted detection system based on GSH@AgNCs and reduced graphene oxide (rGO) with high sensitivity and specificity was developed for GSH detection. Compared with GSH, GSH@AgNCs with weaker affinity and bonding force was quite easier to extrude from the rGO surface when competing against GSH, leading to the obvious change in fluorescence signal. This phenomenon was termed as “a crowding out effect”. Furthermore, the presence of Cys can improve GSH assay sensitivity by enhancing the quenching efficiency of rGO on the GSH@AgNCs. In vitro assay indicated that the efficiency of fluorescence recovery was positively related with GSH concentration in the range from 0 to 10 mM. In addition, the method was employed for real-time monitoring of the dynamic changes in GSH levels regulated by natural drugs. The imaging results showed that the natural compound 3 (C3) can downregulate GSH levels in HepG2 cells, which was accompanied by reactive oxygen species (ROS) release and apoptosis induction. Finally, the method was used to monitor the change of GSH levels in serum samples with chronic hepatitis B (CHB) infection. The results demonstrated that the occurrence and development of CHB may be positively correlated with GSH levels to some extent. Overall, the above results demonstrate the potential application of this new nanosystem in anticancer natural drug screening and clinical assay regarding GSH levels.

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Label-free fluorometric detection of chymotrypsin activity using graphene oxide/nucleic-acid-stabilized silver nanoclusters hybrid materials

Cited by 24 publications

References 42 publications

Activatable endoplasmic reticulum-targeted NIR fluorescent probe with a large Stokes shift for detecting and imaging chymotrypsin

Activatable endoplasmic reticulum-targeted NIR fluorescent probe with a large Stokes shift for detecting and imaging chymotrypsin

A Novel Fluorescence Aptasensor Based on Magnetic Beads/Gold Nanoparticles/DNA-Stabilized Silver Nanoclusters for Detection of Salmonella Typhimurium

Endogenous Cys-Assisted GSH@AgNCs-rGO Nanoprobe for Real-Time Monitoring of Dynamic Change in GSH Levels Regulated by Natural Drug

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