Label-free fluorometric assay for cytochrome c in apoptotic cells based on near infrared Ag2S quantum dots

Synthesis of Ag2S colloidal solutions in D2O heavy water

“…45,46 This weak absorption peak is absent in the absorption spectra of Ag 2 S QDs with a size more than 8.7 nm. Considering a large experimental data array, [47][48][49] the absorption spectra of colloidal Ag 2 S QDs do not contain a peak corresponding to the ground state exciton absorption. It can be assumed that the considerable contribution to the absorption band of colloidal QDs is associated with the nonstoichiometry of silver sulde, 36 which is always accompanied by impurity absorption and leads to the absence of features in the absorption spectrum.…”

A simple fluorescence assay for trypsin through a protamine-induced carbon quantum dot-quenching aggregation platform

“…For Try detection, the total volume of the testing solution was made to be 200 mL. Different amounts (2,4,8,12,16,20,24,28,32, 36 and 40 mL) of 10 mg mL À1 Try stock solution were added to CQDs/Pro solutions prepared from 20 mL of 200 mg mL À1 Pro and 400 mg mL À1 CQDs with the nal CQDs and Pro concentrations of 20 and 40 mg mL À1 . Fluorescent measurements were operated before and aer the incubation of Try in CQDs/Pro at 37 C for 90 min, respectively.…”

“…1,2 Trypsin (Try) is one of the most vital digestive proteases produced in pancreatic acinar cells, and can catalyze the hydrolysis of peptides mainly found at the C-terminus of lysine and arginine residues. [3][4][5] Abnormal Try activity will be reected in pancreatic function and the corresponding pathological changes of the human body. In general, Try is a biomarker for several diseases, such as acute pancreatitis, cystic brosis, and pancreatic cancer.…”

Visualizing Mitochondrial Form and Function within the Cell

“…The most well described mitochondrial signaling molecules are cytochrome c, calcium, reactive oxygen species (ROS), and the redox pairs discussed above, NAD + /NADH and NADP + /NADPH. Several cytochrome c probes have recently been synthesized with the aim of better understanding apoptosis [74][75][76][77][78][79][80][81][82], however, to date, they have been little used to answer specific questions. Future application of these probes to biological questions will be needed to determine the advantages and disadvantages of each for specific experimental models.…”