Label-free fluorescent detection of microRNA-155 based on synthesis of hairpin DNA-templated copper nanoclusters by etching (top-down approach)

Borghei, Yasaman‐Sadat; Hosseini, Morteza; Ganjali, Mohammad Reza; Hosseinkhani, Saman

doi:10.1016/j.snb.2017.03.148

Cited by 79 publications

(34 citation statements)

References 31 publications

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“…In a subsequent study, the incubation time was reduced to 6 h compared to 12 h reported in the first paper. [80] The modified approach not only proved more sensitive (detection limit of 2.2 pM) but could also detect target over a wider range of concentrations (5 pM to 10 nM). Clinical application of both the methods was demonstrated by detecting target nucleic acids in human plasma and saliva samples with high specificity.…”

Section: Other Biological Sensing Conceptsmentioning

confidence: 94%

“…This method could quantify target RNA over a linear range of 50 pM to 10 nM, while a detection limit of 11 pM was realized. In a subsequent study, the incubation time was reduced to 6 h compared to 12 h reported in the first paper . The modified approach not only proved more sensitive (detection limit of 2.2 pM) but could also detect target over a wider range of concentrations (5 pM to 10 nM).…”

Section: The Application Of Dna‐templated Cu‐nps Sensing Of Various Tmentioning

confidence: 95%

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DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

Chen

Shiddiky

et al. 2019

The Chemical Record

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Metal nanoprobes have recently attracted board research interestinr their application in establishing sensing systems due to their unique optical, electrical, physical, and chemical properties. In comparison to gold and silver nanoprobes, analytical platform based on copper nanoprobes (Cu‐NPs) is still in the early stages of development. In this review, we focus on single‐stranded, and double‐stranded DNA capped Cu‐NPs sensing systems which have been designed for various analytes, including metal ions, anions, small molecules, biomolecules (DNA, RNA, and protein, etc.). In addition, the application of Cu‐NPs in biological labeling or bio‐imaging platforms has also been introduced and summarized.

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Section: Other Biological Sensing Conceptsmentioning

confidence: 94%

Section: The Application Of Dna‐templated Cu‐nps Sensing Of Various Tmentioning

confidence: 95%

DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

Chen

Shiddiky

et al. 2019

The Chemical Record

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“…l ‐Cysteine capped CuNCs were synthesized by minor modification of a reported procedure . Here, 175 mg of l ‐cysteine was dissolved in 5 ml 0.4 M NaOH solution by sonication.…”

Section: Methodsmentioning

confidence: 99%

“…Here, 5.0 g of powdered candies were taken and dissolved in 10 ml Millipore water; the resultant solution was centrifuged and filtered to separate the undissolved contents; 100 μl of this extract was used for further analysis . After spiking with QY, soft drink samples were analyzed and recovery was calculated.…”

Section: Methodsmentioning

confidence: 99%

“…Although, copper nanoclusters (CuNCs) are comparatively cheap, their use in the field of sensing has not been much explored . The sensitive and selective sensing potential of CuNCs results from their unique properties such as excellent water solubility, ultrasmall size, bright fluorescence and photostability . CuNCs, therefore were selected as probes for QY sensing; synthesized CuNCs can be stored up to 1 year without any change in their optical properties and can be considered as an economical probe for analysis of QY.…”

Section: Introductionmentioning

confidence: 99%

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Copper nanoclusters: an efficient fluorescence sensing platform for quinoline yellow

et al. 2019

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Fluorescence quenching behavior of artificial food colorant quinoline yellow (QY), on interaction with L-cysteine stabilized copper nanoclusters (L-Cys-CuNCs) is investigated in this work. For this purpose, L-cysteine stabilized CuNCs were synthesized and characterized using various analytical techniques. Results demonstrated that the synthesized probe (size~2 nm) had very promising optical features such as bright blue fluorescence, significant quantum yield and excellent photostability. L-Cys-CuNCs can function as a fluorescence sensor by selectively sensing QY among other yellow colorants, giving a detection limit as low as 0.11 μM. The developed sensor exhibited a linear concentration range from 5.50 to 0.20 μM. The developed fluorescence assay was successfully applied for testing commercial samples, thereby making this sensing strategy significant for quality control of food stuffs.

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Recent advances in nanomaterials‐based optical sensors for detection of various biomarkers (inorganic species, organic and biomolecules)

Harshita

Kailasa

2022

Luminescence

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This review briefly emphasizes the different detection approaches (electrochemical sensors, chemiluminescence, surface‐enhanced Raman scattering), functional nanostructure materials (quantum dots, metal nanoparticles, metal nanoclusters, magnetic nanomaterials, metal oxide nanoparticles, polymer‐based nanomaterials, and carbonaceous nanomaterials) and detection mechanisms. Furthermore, the emphasis of this review is on the integration of functional nanomaterials with optical spectroscopic techniques for the identification of various biomarkers (nucleic acids, glucose, uric acid, oxytocin, dopamine, ascorbic acid, bilirubin, spermine, serotonin, thiocyanate, Pb2+, Cu2+, Hg2+, F−, peptides), and cancer biomarkers (mucin 1, prostate specific antigen, carcinoembryonic antigen, CA15‐3, human epidermal growth factor receptor 2, C‐reactive protein, and interleukin‐6). Analytical characteristics of nanomaterials‐based optical sensors are summarized in the tables, providing the insights of nanomaterials‐based optical sensors for biomarkers detection. Finally, the opportunities and challenges of nanomaterials‐based optical analytical approaches for the detection of various biomarkers (inorganic, organic, biomolecules, peptides and proteins) are discussed.

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Label-free fluorescent detection of microRNA-155 based on synthesis of hairpin DNA-templated copper nanoclusters by etching (top-down approach)

Cited by 79 publications

References 31 publications

DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

Copper nanoclusters: an efficient fluorescence sensing platform for quinoline yellow

Recent advances in nanomaterials‐based optical sensors for detection of various biomarkers (inorganic species, organic and biomolecules)

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