Label-free enrichment of fate-biased human neural stem and progenitor cells

Adams, Tayloria N.G.; Jiang, Alan; Mendoza, Nicolo S.; Ro, Clarissa C.; Lee, Do‐Hyun; Lee, Abraham P.; Flanagan, Lisa A.

doi:10.1016/j.bios.2019.111982

Cited by 23 publications

(22 citation statements)

References 41 publications

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“…Additionally, N-glycans on the surface of the membrane are indicators of whether an NSPC will differentiate into an astrocyte or a neuron [32]. Recently, a two-element microfluidic device with a hydrophoresis module upstream of a DEP module has been developed by Jiang et al for the enrichment of astrocytebiased progenitor cells from murine and human NSPCs (Figure 3A) [33,34]. In 2019, Liu et al characterized and separated NSPCs using DC-iDEP with fields ranging from 10 8 to 10 9 V/m 2 , concentrated on sawtooth electrode geometries based on their fate−bias by quantifying the ratio of electrokinetic mobility to DEP mobility [35].…”

Section: Neural Stem Cellsmentioning

confidence: 99%

A review: Dielectrophoresis for characterizing and separating similar cell subpopulations based on bioelectric property changes due to disease progression and therapy assessment

Duncan

Davalos

2021

Electrophoresis

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This paper reviews the use of dielectrophoresis for high-fidelity separations and characterizations of subpopulations to highlight the recent advances in the electrokinetic field as well as provide insight into its progress toward commercialization. The role of cell subpopulations in heterogeneous clinical samples has been studied to deduce their role in disease progression and therapy resistance for instances such as cancer, tissue regeneration, and bacterial infection. Dielectrophoresis (DEP), a label-free electrokinetic technique, has been used to characterize and separate target subpopulations from mixed samples to determine disease severity, cell stemness, and drug efficacy. Despite its high sensitivity to characterize similar or related cells based on their differing bioelectric signatures, DEP has been slowly adopted both commercially and clinically. This review addresses the use of dielectrophoresis for the identification of target cell subtypes in stem cells, cancer cells, blood cells, and bacterial cells dependent on cell state and therapy exposure and addresses commercialization efforts in light of its sensitivity and future perspectives of the technology, both commercially and academically.

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Section: Neural Stem Cellsmentioning

confidence: 99%

A review: Dielectrophoresis for characterizing and separating similar cell subpopulations based on bioelectric property changes due to disease progression and therapy assessment

Duncan

Davalos

2021

Electrophoresis

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“…The cells were washed 3 times before resuspending in DEP buffer at 1 x 10 6 cells/mL. First, a visual DEP screening of hMSC heterogeneity was completed with a quadrupole device (fabricated using previously published techniques [6,23] ; electrode dimensions: 50 μm wide with 200 μm spacing). At select frequencies and 10 Vpp cell movement was recorded.…”

Section: Dep Characterizationmentioning

confidence: 99%

“…In these stem cell therapies, the heterogeneity of BM-hMSCs and UC-hMSCs impacted patient outcomes. One method proven successful for reducing the heterogeneity of stem cells is the implementation of dielectrophoresis (DEP) on microfluidic devices [6] .…”

Section: Introductionmentioning

confidence: 99%

Electrical signature of heterogeneous human mesenchymal stem cells

Tsai

Vyas

Crowell

et al. 2021

Preprint

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Human mesenchymal stem cells (hMSCs) have gained traction in transplantation therapy due to their immunomodulatory, paracrine, immune-evasive, and multipotent differentiation potential. Given the heterogeneous nature of hMSCs, therapeutic treatments and robust in vivo and in vitro experiments require additional biomarkers to ensure reproducibility when using these stem cells. In this work, we utilized dielectrophoresis (DEP), a label-free electrokinetic phenomenon, to investigate and quantify the heterogeneity of hMSCs derived from the bone marrow (BM) and adipose tissue (AD). Through computer simulation, we identified that the transient slope of the DEP force spectra can be used as a metric of heterogeneity. The electrical properties of BM-hMSCs were compared to homogeneous mouse fibroblasts (NIH-3T3), human fibroblasts (WS1), and human embryonic kidney cells (HEK-293). BM-hMSCs DEP profile was most different from HEK-293 cells. We compared the DEP profiles of BM-hMSCs and AD-hMSCs and found they have similar membrane capacitances, differing cytoplasm conductivity, and transient slopes. Inducing both populations to differentiate into adipocyte and osteocyte cells revealed they behave differently in response to differentiation-inducing cytokines. Histology and RT-qPCR analyses of the differentiation-related genes revealed differences in heterogeneity between BM-hMSCs and AD-hMSCs. The differentiation profiles correlate well with the DEP profiles developed and indicate that these BM-hMSCs have higher differentiation potential than AD-hMSCs. Our results demonstrate using DEP, membrane capacitance, cytoplasm conductivity, and transient slope can uniquely characterize the inherent heterogeneity of hMSCs to guide robust and reproducible stem cell transplantation therapies.

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“…EIS and DEP are label-free electric field-based technique that can exploit the differences amongst the dielectric properties of cancer cells [ 78 ]. DEP is similar to EIS because cell polarization is utilized to examine the dielectric properties of cells and has been used for bioparticle characterization [ 79 ], manipulation [ 80 ], and sorting [ 81 ]. DEP has been implemented to isolate rare CTCs [ 53 ].…”

Section: Applications Of Impedance Spectroscopy For Cancer Cellsmentioning

confidence: 99%

“…The cell patterning capabilities of EIS combined with DEP can be employed to build complex 3D microenvironments that represent in vivo cancer conditions. Another aspect of DEP is its implementation to continuously sort a variety of cells [ 81 , 101 , 102 , 103 , 104 ], thus an exciting possibility is to sort CSCs from non-CSCs prior to impedance monitoring. These techniques can also be used for drug optimization and monitoring as well as to understand the molecular and environmental driving forces involved in plasticity.…”

Section: Future Trends In Monitoring Cancer Cell Dynamicsmentioning

confidence: 99%

Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells

et al. 2020

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Electrical impedance spectroscopy (EIS) is an electrokinetic method that allows for the characterization of intrinsic dielectric properties of cells. EIS has emerged in the last decade as a promising method for the characterization of cancerous cells, providing information on inductance, capacitance, and impedance of cells. The individual cell behavior can be quantified using its characteristic phase angle, amplitude, and frequency measurements obtained by fitting the input frequency-dependent cellular response to a resistor–capacitor circuit model. These electrical properties will provide important information about unique biomarkers related to the behavior of these cancerous cells, especially monitoring their chemoresistivity and sensitivity to chemotherapeutics. There are currently few methods to assess drug resistant cancer cells, and therefore it is difficult to identify and eliminate drug-resistant cancer cells found in static and metastatic tumors. Establishing techniques for the real-time monitoring of changes in cancer cell phenotypes is, therefore, important for understanding cancer cell dynamics and their plastic properties. EIS can be used to monitor these changes. In this review, we will cover the theory behind EIS, other impedance techniques, and how EIS can be used to monitor cell behavior and phenotype changes within cancerous cells.

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Label-free enrichment of fate-biased human neural stem and progenitor cells

Cited by 23 publications

References 41 publications

A review: Dielectrophoresis for characterizing and separating similar cell subpopulations based on bioelectric property changes due to disease progression and therapy assessment

A review: Dielectrophoresis for characterizing and separating similar cell subpopulations based on bioelectric property changes due to disease progression and therapy assessment

Electrical signature of heterogeneous human mesenchymal stem cells

Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells

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