Label-free electrochemiluminescent detection of transcription factors with hybridization chain reaction amplification

Xiong, Yunfang; Lin, Lin; Zhang, Xiaojun; Wang, Guangfeng

doi:10.1039/c6ra00701e

Cited by 13 publications

(7 citation statements)

References 82 publications

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“…[53] Figure 7A depicts one configuration that applies the redox-labeled nucleic acid 21 as aTFO strand for the electrochemical detection of the duplex DNAanalyte 22/23. As the concentration of the duplex analyte is increased, the voltammetric readout signal decreases.T his sensing platform has been used to detect HIV-1 strains [53] and to analyze sequencespecific double strands, [54] transcription factors, [55] and adenosine. As the concentration of the duplex analyte is increased, the voltammetric readout signal decreases.T his sensing platform has been used to detect HIV-1 strains [53] and to analyze sequencespecific double strands, [54] transcription factors, [55] and adenosine.…”

Section: Triplex-driven Electrochemical Sensorsmentioning

confidence: 99%

“…[53] Theflexibility of the single strand leads to effective electrical communication between the electrode and methylene blue (MB), resulting in an intense voltammetric response.Inthe presence of the target duplex DNA 22/ 23,the formation of the triplex between the target duplex and the redox-labeled TFO 21 leads to the rigidification of the redox-labeled units,t hus hindering the electrical contact between the redox label and the electrode support and suppressing the signal (lower voltammetric response). As the concentration of the duplex analyte is increased, the voltammetric readout signal decreases.T his sensing platform has been used to detect HIV-1 strains [53] and to analyze sequencespecific double strands, [54] transcription factors, [55] and adenosine. [56] At riplex-based sensing platform that includes ac lamplike mechanism and provides a" signal on" electrochemical readout is depicted in Figure 7B.…”

Section: Triplex-driven Electrochemical Sensorsmentioning

confidence: 99%

“…As the concentration of the duplex analyte is increased, the voltammetric readout signal decreases. This sensing platform has been used to detect HIV‐1 strains and to analyze sequence‐specific double strands, transcription factors, and adenosine…”

Section: Dna Triplexes For Sensing Applicationsmentioning

confidence: 99%

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Triplex DNA Nanostructures: From Basic Properties to Applications

et al. 2017

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Triplex nucleic acids have recently attracted interest as part of the rich "toolbox" of structures used to develop DNA-based nanostructures and materials. This Review addresses the use of DNA triplexes to assemble sensing platforms and molecular switches. Furthermore, the pH-induced, switchable assembly and dissociation of triplex-DNA-bridged nanostructures are presented. Specifically, the aggregation/deaggregation of nanoparticles, the reversible oligomerization of origami tiles and DNA circles, and the use of triplex DNA structures as functional units for the assembly of pH-responsive systems and materials are described. Examples include semiconductor-loaded DNA-stabilized microcapsules, DNA-functionalized dye-loaded metal-organic frameworks (MOFs), and the pH-induced release of the loads. Furthermore, the design of stimuli-responsive DNA-based hydrogels undergoing reversible pH-induced hydrogel-to-solution transitions using triplex nucleic acids is introduced, and the use of triplex DNA to assemble shape-memory hydrogels is discussed. An outlook for possible future applications of triplex nucleic acids is also provided.

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Section: Triplex-driven Electrochemical Sensorsmentioning

confidence: 99%

Section: Triplex-driven Electrochemical Sensorsmentioning

confidence: 99%

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Triplex DNA Nanostructures: From Basic Properties to Applications

et al. 2017

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“…Das ausgelesene voltammetrische Signal nimmt also mit zunehmender Konzentration des Duplex‐Analyten ab. Die Sensorplattform wurde zum Nachweis von HIV‐Stämmen sowie zur Analyse von sequenzspezifischen Doppelsträngen, Transkriptionsfaktoren und Adenosin verwendet.…”

Section: Dna‐triplexstrukturen Für Die Sensorikunclassified

Triplex‐DNA‐Nanostrukturen: von grundlegenden Eigenschaften zu Anwendungen

Cecconello

Idili

et al. 2017

Angewandte Chemie

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Triplex‐Nukleinsäuren sind ein Teil des umfangreichen “Struktur‐Werkzeugkastens”, der für die Entwicklung von Nanostrukturen und Materialien auf DNA‐Basis eingesetzt wird. Dieser Aufsatz behandelt die Verwendung von DNA‐Triplexen für die Konstruktion von Sensorplattformen und molekularen Schaltern. Daneben behandeln wir die pH‐induzierte, schaltbare Assoziation und Dissoziation von Triplex‐DNA‐verbrückten Nanostrukturen. Dazu gehören die Aggregation/Deaggregation von Nanopartikeln, die reversible Oligomerisierung von Origamikacheln oder DNA‐Zyklen und die Verwendung von Triplex‐DNA‐Strukturen als Funktionseinheiten zum Aufbau von pH‐responsiven Systemen und Materialien. Beispiele hierfür sind Halbleiter‐beladene DNA‐stabilisierte Mikrokapseln und DNA‐funktionalisierte Farbstoff‐beladene Metall‐organische Gerüste (MOFs) sowie die pH‐induzierte Freisetzung der Beladungen. Auch stimuliresponsive Hydrogele auf DNA‐Basis, die unter Verwendung von Triplex‐Nukleinsäuren reversible pH‐induzierte Hydrogel‐zu‐Lösung‐Übergänge durchlaufen und die Verwendung von Triplex‐DNA zum Herstellen von Formgedächtnis‐Hydrogelen werden behandelt. Schließlich wird ein Ausblick auf zukünftige Anwendungen und Perspektiven für Triplex‐Nukleinsäuren gegeben.

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“…Ru(phen) 3 2+ has the characteristic of being specifically embedded in the groove of dsDNA and acting as an ECL indicator. This character has been used to prepare many effective ECL sensors [24–26]. Most of these sensors produced the dsDNA on the electrode surface directly.…”

Section: Introductionmentioning

confidence: 99%

High Sensitive Electrochemiluminescence Biosensor Based on Ru(phen)₃²⁺‐loaded Double Strand DNA as Signal Tags use to Detect DNA Methyltransferase Activity

et al. 2021

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DNA methyltransferase (DNA MTase) can act as biomarker for many diseases and it is important to develop some new methods for sensitive detection of DNA MTase. In this work, a highly efficient electrochemiluminescence (ECL) sensor had been designed for detection of DNA MTase based on Ru(phen)32+ loaded double strand DNA (dsDNA‐ Ru(phen)32+) as signal tags. Ru(phen)32+ had been efficiently embed in the dsDNA produced through a simple hybridization chain reaction. First, a hairpin probe was designed, which can be specifically recognized by Dam MTase and modified with ‐SH at one end. It was modified on the surface of gold electrode by ‐SH as an immobilization probe (IP). This IP will be methylated in the present of Dam MTase and digested by DpnI following. Results in the release of capture probe (CP) which remains on the surface of gold electrode. The CP can hybridize with the single stand part of the dsDNA‐ Ru(phen)32+ and make the immobilization of ECL tags on the electrode surface, which results in a strong ECL signals detected. However, without the effect of Dam MTase, the hairpin structure of IP remains stable and cannot capture signal tags, and can only detecte weak ECL signals. The biosensor can detect the activity of Dam MTase in the concentration range of 0.01 U/mL to 20 U/mL with the ECL intensity and the logarithm of the concentration have a linear relationship, and the detection limit is calculated to be 7.6 mU/mL. The developed sensor has the ability to specifically detect Dam MTase, which can be differentiated from other types of DNA MTase. In addition, the designed method has good applicability to detect Dam MTase activity in serum samples and been applied to detect its inhibitor with high efficiency.

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Label-free electrochemiluminescent detection of transcription factors with hybridization chain reaction amplification

Abstract: Label-free and efficient ECL strategy for detection of NF-κB based on the HCR signal amplification.

Cited by 13 publications

References 82 publications

Triplex DNA Nanostructures: From Basic Properties to Applications

Triplex DNA Nanostructures: From Basic Properties to Applications

Triplex‐DNA‐Nanostrukturen: von grundlegenden Eigenschaften zu Anwendungen

High Sensitive Electrochemiluminescence Biosensor Based on Ru(phen)₃²⁺‐loaded Double Strand DNA as Signal Tags use to Detect DNA Methyltransferase Activity

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Label-free electrochemiluminescent detection of transcription factors with hybridization chain reaction amplification

Abstract: Label-free and efficient ECL strategy for detection of NF-κB based on the HCR signal amplification.

Cited by 13 publications

References 82 publications

Triplex DNA Nanostructures: From Basic Properties to Applications

Triplex DNA Nanostructures: From Basic Properties to Applications

Triplex‐DNA‐Nanostrukturen: von grundlegenden Eigenschaften zu Anwendungen

High Sensitive Electrochemiluminescence Biosensor Based on Ru(phen)32+‐loaded Double Strand DNA as Signal Tags use to Detect DNA Methyltransferase Activity

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Product

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High Sensitive Electrochemiluminescence Biosensor Based on Ru(phen)₃²⁺‐loaded Double Strand DNA as Signal Tags use to Detect DNA Methyltransferase Activity