Label-Free Electrochemical Sensor Based on Manganese Doped Titanium Dioxide Nanoparticles for Myoglobin Detection: Biomarker for Acute Myocardial Infarction

Abstract

A label free electrochemical sensor based on pure titanium oxide and manganese (Mn)-doped titanium oxide (TiO2) nanoparticles are fabricated and characterized for the sensitive detection of myoglobin (Mb) levels to analyze the cardiovascular infarction. Pristine and Mn-doped TiO2 nanoparticles were synthesized via the sol-gel method and characterized in order to understand their structure, morphologies, composition and optical properties. The structural properties revealed that the pure- and doped-TiO2 nanoparticles possess different TiO2 planes. FTIR studies confirm the formation of metal oxide nanoparticles by exhibiting a well-defined peak in the range of 600–650 cm−1. The values of the optical band gap, estimated from UV-Vis spectroscopy, are decreased for the Mn-doped TiO2 nanoparticles. UV-Vis spectra in the presence of myoglobin (Mb) indicated interaction between the TiO2 nanoparticles and myoglobin. The SPE electrodes were then fabricated by printing powder film over the working electrode and tested for label-free electrochemical detection of myoglobin (Mb) in the concentration range of 0–15 nM Mb. The fabricated electrochemical sensor exhibited a high sensitivity of 100.40 μA-cm−2/nM with a lowest detection limit of 0.013 nM (0.22 ng/mL) and a response time of ≤10 ms for sample S3. An interference study with cyt-c and Human Serum Albumin (HSA) of the sensors show the selective response towards Mb in 1:1 mixture.

Sustainable chemistry approach for the preparation of bluish green emissive copper nanoclusters fromJusticia adhatodaleaves extract: a facile analytical approach for the sensing of myoglobin andl-thyroxine

“…S10 of the ESI†), which gives the LOD of 7.4 nM. Comparisons of the analytical features of the J. adhatoda– Cu NC-based fluorescence method with reported analytical methods for the sensing of myoglobin 28–37 and l -thyroxine 12,13,38–44 are shown in Tables 1 and 2. It is clear that the J. adhatoda– Cu NC-based fluorescence method exhibits much more sensitivity than that of other analytical techniques for assaying myoglobin 28–30 and l -thyroxine.…”

Zero-Biased Photoelectrochemical Detection of Cardiac Biomarker Myoglobin Based on CdSeS/ZnS Quantum Dots and Barium Titanate Perovskite

“…The analytical parameters obtained with the anti-Mb/CdSeS/ZnSQDs/BTO/FTO were equated with other platforms to detect Mb reported in the literature. Table S1 [ 3 , 5 , 11 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ] shows the analytical characteristics of immunosensors for Mb and a comparison of materials with the platform proposed. This table shows several matrices used for the detection of Mb.…”

Nanoparticles in the diagnosis and treatment of vascular aging and related diseases

“…A label-free electrochemical biosensor can be used for the effective and sensitive detection of myoglobin levels and the assessment of MI phases. 230 Monoclonal anti-myoglobin antibody-coated polyethylenimine (PEI)-AuNPs have the ability for quantitative detection of myoglobin, with a detection range from 9.96 ng/mL to 72.8 ng/mL and a detection limit of 6.29 ng/mL. 231 Anodiamonds and hydrogen-substituted graphdiyne mixture (HsGDY@NDs) have excellent sensing performance for myoglobin and cTnI detection, with low detection limits of 9.04 fg/mL and 6.29 fg/mL, respectively.…”