Label-free Electrochemical Impedance Spectroscopy Aptasensor for Ultrasensitive Detection of Lung Cancer Biomarker Carcinoembryonic Antigen

Abstract

In this work, an integrated electrode system consisting of a graphene working electrode, a carbon counter electrode and an Ag/AgCl reference electrode was fabricated on an FR-4 glass fiber plate by a polyethylene self-adhesive mask stencil method combined with a manual screen printing technique. The integrated graphene electrode was used as the base electrode, and AuNPs were deposited on the working electrode surface by cyclic voltammetry. Then, the carcinoembryonic antigen aptamer was immobilized using the sulfhydryl self-assembly technique. The sensor uses [Fe(CN)6]3−/4− as a redox probe for label free detection of carcinoembryonic antigen based on the impedance change caused by the difference in electron transfer rate before and after the binding of carcinoembryonic antigen aptamer and the target carcinoembryonic antigen. The results showed a good linear relationship when the CEA concentration is in the range of 0.2–15.0 ng/ml. The detection limit was calculated to be 0.085 ng/ml (S/N = 3).

Recent advancement in the detection of potential cancer biomarkers using the nanomaterial integrated electrochemical sensing technique: a detailed review

“…Recently, Wang et al in 2021 developed a label-free electrochemical impedimetric aptasensor for lung cancer detection based on the CEA cancer biomarker using a Au NP decorated graphene material. 183 For the working electrode surface modification, the graphene electrode was used as the base electrode and gold nanoparticles were decorated using the electrodeposition cyclic voltammetry technique. An optimized concentration of the aptamer was immobilized over the nanoparticle decorated graphene electrode and electrochemical sensing response was evaluated using the EIS technique.…”

Construction of Electrochemical Aptamer Sensor Based on Pt-Coordinated Titanium-Based Porphyrin MOF for Thrombin Detection

“…Among the many electrode modification materials, MOFs have received a lot of attention as a new type of nanomaterials, which are crystalline materials assembled with metal centers and organic ligands (Duan et al, 2019). Due to the large number of combinations between metal centers and organic ligands, many new MOFs can be synthesized by adjusting metal centers, organic ligands and reaction conditions (Cao et al, 2019;Karimi-Maleh et al, 2021;Wang et al, 2021). These advantages have led to the application of MOFs nanomaterials in gas separation, energy storage and electrochemical analysis.…”

Graphene-Assisted Electrochemical Sensor for Detection of Pancreatic Cancer Markers

“…As a single-atom planar two-dimensional crystal, graphene's unusual electronic structure, specific electronic properties and excellent electron transport capacity make it uniquely advantageous in the field of high-sensitivity detection (Akbari jonous et al, 2019). Graphene, on the one hand, combines the redox properties of some bioelectrically active molecules and can detect the target molecule by its redox reaction on the electrode surface and the corresponding current signal (Coroş et al, 2019;Wang et al, 2021b). On the other hand, the bipolarity of graphene can be easily monitored in resistive sensors (Krishnan et al, 2019).…”