Label-free electrochemical immunosensor based on electrodeposited Prussian blue and gold nanoparticles for sensitive detection of citrus bacterial canker disease

Haji-Hashemi, Hedieh; Habibi, Mohammad Mahdi; Safarnejad, Mohammad Reza; Norouzi, Parviz; Ganjali, Mohammad Reza

doi:10.1016/j.snb.2018.07.148

Cited by 38 publications

(22 citation statements)

References 45 publications

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“…[19][20][21] The resulting user-friendly cortisol sensor, integrating the MIP recognition and the built-in PB-transduction element, thus relies on chronoamperometric measurements (CA) of the PB oxidation current. [19,22] The selective binding of cortisol within the imprinted cavities, leads to blocking of the PB electron transfer pathways and thus to a decreased PB oxidation current. The extent of such current diminution reflects the sweat cortisol concentration and can thus serve as the analytical signal (Figure 1B).…”

Section: Introductionmentioning

confidence: 99%

Touch‐Based Stressless Cortisol Sensing

et al. 2021

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Tracking fluctuations of the cortisol level is important in understanding the body's endocrine response to stress stimuli. Traditional cortisol sensing relies on centralized laboratory settings, while wearable cortisol sensors are limited to slow and complex assays. Here, a touch‐based non‐invasive molecularly imprinted polymer (MIP) electrochemical sensor for rapid, simple, and reliable stress‐free detection of sweat cortisol is described. The sensor readily measures fingertip sweat cortisol via highly selective binding to the cortisol‐imprinted electropolymerized polypyrrole coating. The MIP network is embedded with Prussian blue redox probes that offer direct electrical signaling of the binding event to realize sensitive label‐free amperometric detection. Using a highly permeable sweat‐wicking porous hydrogel, instantaneously secreted fingertip sweat can be conveniently and rapidly collected without any assistance. By eliminating time lags, such rapid (3.5 min) fingertip assay enables the capture of sharp variations in cortisol levels, compared to previous methods. Such advantages are demonstrated by tracking cortisol response in short cold‐pressor tests and throughout day‐long circadian rhythm, along with gold‐standard immunoassay validation. A stretchable epidermal MIP sensor is also described for directly tracking cortisol in exercise‐induced sweat. The rapid touch‐based cortisol sensor offers an attractive, accessible, stressless avenue for quantitative stress management.

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Section: Introductionmentioning

confidence: 99%

Touch‐Based Stressless Cortisol Sensing

et al. 2021

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“…citri. ; Xcc) based on the directly recognizing the effector protein PthA was developed in [81]. The sensor fabrication involved sequential electro-deposition of PB and AuNPs on multiwall carbon nanotubes (MWCNTs)-ionic liquid nanocomposite modified glassy carbon electrode.…”

Section: Substrate Flow Directionmentioning

confidence: 99%

Sensing Methodologies in Agriculture for Monitoring Biotic Stress in Plants Due to Pathogens and Pests

Kashyap

Kumar

2021

Inventions

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Reducing agricultural losses is an effective way to sustainably increase agricultural output efficiency to meet our present and future needs for food, fiber, fodder, and fuel. Our ever-improving understanding of the ways in which plants respond to stress, biotic and abiotic, has led to the development of innovative sensing technologies for detecting crop stresses/stressors and deploying efficient measures. This article aims to present the current state of the methodologies applied in the field of agriculture towards the detection of biotic stress in crops. Key sensing methodologies for plant pathogen (or phytopathogen), as well as herbivorous insects/pests are presented, where the working principles are described, and key recent works discussed. The detection methods overviewed for phytopathogen-related stress identification include nucleic acid-based methods, immunological methods, imaging-based techniques, spectroscopic methods, phytohormone biosensing methods, monitoring methods for plant volatiles, and active remote sensing technologies. Whereas the pest-related sensing techniques include machine-vision-based methods, pest acoustic-emission sensors, and volatile organic compound-based stress monitoring methods. Additionally, Comparisons have been made between different sensing techniques as well as recently reported works, where the strengths and limitations are identified. Finally, the prospective future directions for monitoring biotic stress in crops are discussed.

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“…The diameter of the semicircle represents the charge-transfer resistance (R ct ) at the electrode surface and a straight line shows the diffusion resistance. 46,47 EIS measurements were recorded at an AC voltage amplitude of +10 mV and frequency in a range from 0.01 Hz to 100 kHz, superimposed on a direct current (DC) potential of +0.13 V. Figure 2 shows the Nyquist plots of impedance spectra in a solution of 0.1 M PBS, containing 5 mM [Fe(CN) 6 ] 3-/4in 0.1 M KCl (pH 7.4) obtained at each electrode surface modification step. Figure 2 (blue curve) represents the electrochemical impedance of the bare SPCE which is semicircle.…”

Section: Eis Spectra Of Surface-modified Electrodesmentioning

confidence: 99%

<p>An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker</p>

Maghsoudi¹,

Hassani²,

Akmal³

et al. 2020

IJN

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In the present study, a highly sensitive and simple electrochemical (EC) aptasensor for the detection of serpin A12 as a novel biomarker of diabetes was developed on a platform where flower-like gold microstructures (FLGMs) are electrodeposited onto a disposable screen-printed carbon electrode. Meanwhile, serpin A12-specific thiolated aptamer was covalently immobilized on the FLGMs. Methods: The electrochemical activity of a fabricated aptasensor under various conditions were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Aptamer concentration, deposition time, self-assembly time, and incubation time were optimized for assay of serpin A12. The differential pulse voltammetry (DPV) was implemented for quantitative detection of serpin A12 in K 3 [Fe (CN) 6 ]/K 4 [Fe (CN) 6 ] solution (redox probe). Results: The label-free aptasensor revealed a linear range of serpin A12 concentration (0.039-10 ng/mL), detection limit of 0.020 ng/mL (S/N=3), and 0.031 ng/mL in solution buffer and plasma, respectively. Conclusion: The results indicate that this aptasensor has a high sensitivity, selectivity, stability, and acceptable reproducibility for detection of serpin A12 in diabetic patients.

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Label-free electrochemical immunosensor based on electrodeposited Prussian blue and gold nanoparticles for sensitive detection of citrus bacterial canker disease

Cited by 38 publications

References 45 publications

Touch‐Based Stressless Cortisol Sensing

Touch‐Based Stressless Cortisol Sensing

Sensing Methodologies in Agriculture for Monitoring Biotic Stress in Plants Due to Pathogens and Pests

<p>An Electrochemical Aptasensor Platform Based on Flower-Like Gold Microstructure-Modified Screen-Printed Carbon Electrode for Detection of Serpin A12 as a Type 2 Diabetes Biomarker</p>

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