Label-Free Electrochemical Detection of Tetracycline by an Aptamer Nano-Biosensor

Zhang, Juankun; Wu, Yongjin; Zhang, Binbin; Li, Min; Jia, Shiru; Jiang, Shuhai; Zhou, Hao; Zhang, Yi; Zhang, Chaozheng; Turner, Anthony P. F.

doi:10.1080/00032719.2012.670784

Cited by 32 publications

(13 citation statements)

References 23 publications

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“…In recent years, porous silicon (PSi) has emerged as a promising nanomaterial for the design of different optical label-free biosensing platforms, owing to its large surface area, versatile chemistry and straightforward fabrication [22][23][24][25][26][27][28][29][30][31][32][33][34][35] . PSi-based interferometers, in which a change in refractive index of the solution contained within the porous nanostructure can be measured, are most common and the technique is often referred to as reflective interferometric Fourier transform spectroscopy (RIFTS) 30,33,36,37 .…”

Section: Introductionmentioning

confidence: 99%

Label-Free Optical Biosensors Based on Aptamer-Functionalized Porous Silicon Scaffolds

et al. 2015

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A proof-of-concept for a label-free and reagentless optical biosensing platform based on nanostructured porous silicon (PSi) and aptamers is presented in this work. Aptamers are oligonucleotides (single-stranded DNA or RNA) that can bind their targets with high affinity and specificity, making them excellent recognition elements for biosensor design. Here we describe the fabrication and characterization of aptamer-conjugated PSi biosensors, where a previously characterized his-tag binding aptamer (6H7) is used as model system. Exposure of the aptamer-functionalized PSi to the target proteins as well as to complex fluids (i.e., bacteria lysates containing target proteins) results in robust and well-defined changes in the PSi optical interference spectrum, ascribed to specific aptamer-protein binding events occurring within the nanoscale pores, monitored in real time. The biosensors show exceptional stability and can be easily regenerated by a short rinsing step for multiple biosensing analyses. This proof-of-concept study demonstrates the possibility of designing highly stable and specific label-free optical PSi biosensors, employing aptamers as capture probes, holding immense potential for application in detection of a broad range of targets, in a simple yet reliable manner.

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Section: Introductionmentioning

confidence: 99%

Label-Free Optical Biosensors Based on Aptamer-Functionalized Porous Silicon Scaffolds

et al. 2015

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“…Therefore, it is not possible to assess whether the detection method or the K D value might be the limiting factor for the obtained sensitivity. Thus, further [86] studies using aptamer/target pairs with known dissociation constants in combination with PSi-based impedance assays may result in promising detection schemes, which are highly sensitive yet simple.…”

Section: Electrochemical Biosensorsmentioning

confidence: 97%

“…Other biosensors based on PSi are mainly electrochemical, relying on PSi semiconductor characteristics [82]. Examples include voltammetric approaches [83] as well as amperometric [84], potentiometric characterization [85] and impedance-based sensors [86].…”

Section: Porous Silicon-based Biosensorsmentioning

confidence: 99%

Porous Silicon Biosensors Employing Emerging Capture Probes

Urmann

Tenenbaum

Walter

et al. 2015

Electrochemically Engineered Nanoporous Materials

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The application of porous silicon (PSi) for biosensing was first described by Thust et al. in 1996, demonstrating a potentiometric biosensor for the detection of penicillin. However, only in the past decade PSi has established as a promising nanomaterial for label-free biosensing applications. This chapter focuses on the integration of new emerging capture probes with PSi-based biosensing schemes. An overview of natural and synthetic receptors and their advantageous characteristics for the potential application in PSi biosensors technology is presented. We also review and discuss several examples, which successfully combine these new bioreceptors with PSi optical and electrochemical transducers, for label-free biosensing.

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“…A label-free electrochemical nanobiosensor was developed by Zhang et al [ 74 ] for the detection of tetracycline. For the construction of the biosensor, a nanoporous silicon (PS) chip was firstly functionalized with amino groups through silanization in 3-aminopropyltriethoxysilane.…”

Section: Electrochemical Biosensors For Antimicrobial Drug Residuementioning

confidence: 99%

An Overview on Recent Progress in Electrochemical Biosensors for Antimicrobial Drug Residues in Animal-Derived Food

Majdinasab

Yaqub

Rahim

et al. 2017

Sensors

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Anti-microbial drugs are widely employed for the treatment and cure of diseases in animals, promotion of animal growth, and feed efficiency. However, the scientific literature has indicated the possible presence of antimicrobial drug residues in animal-derived food, making it one of the key public concerns for food safety. Therefore, it is highly desirable to design fast and accurate methodologies to monitor antimicrobial drug residues in animal-derived food. Legislation is in place in many countries to ensure antimicrobial drug residue quantities are less than the maximum residue limits (MRL) defined on the basis of food safety. In this context, the recent years have witnessed a special interest in the field of electrochemical biosensors for food safety, based on their unique analytical features. This review article is focused on the recent progress in the domain of electrochemical biosensors to monitor antimicrobial drug residues in animal-derived food.

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Label-Free Electrochemical Detection of Tetracycline by an Aptamer Nano-Biosensor

Cited by 32 publications

References 23 publications

Label-Free Optical Biosensors Based on Aptamer-Functionalized Porous Silicon Scaffolds

Label-Free Optical Biosensors Based on Aptamer-Functionalized Porous Silicon Scaffolds

Porous Silicon Biosensors Employing Emerging Capture Probes

An Overview on Recent Progress in Electrochemical Biosensors for Antimicrobial Drug Residues in Animal-Derived Food

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